A Comparison of Three Different Methods for the Determination of Estrogen Receptors in Human Breast Cancer

Abstract

Forty-seven cases of breast cancer were analyzed for estrogen receptors (ERs) by three different techniques: (1) a biochemical assay using isoelectric focusing in polyacrylamide gel; (2) a monoclonal ER immunocytochemical assay (ERICA, Abbott Laboratories Diagnostics Division), and (3) a polyclonal antiestradiol immunohistochemical technique using formalin-fixed tissue. A high level of agreement (85%) was noted between the biochemical assay and ERICA. The ERICA-processed slides showed minimal background staining and were easy to interpret. Compared with the biochemical assay, ERICA achieved a positive predictive value of 86% and a negative predictive value of 82%. In contrast, results from the polyclonal antiestradiol histologic technique did not correlate with biochemical results and showed positive and negative predictive values of 61% and 25%, respectively. The authors conclude that the ERICA technique should prove to be a valuable adjunct to traditional biochemical ER assays.

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“…Several evaluation systems have been proposed in the literature (9)(10)(11)(12) and involve positive cell population and staining intensity. To quantify the results by immunohistochemistry, the percentage of positive cells among 100 to 500 breast cancer cells should ideally be counted for the assessment.…”

“…Concordance rates between biochemical assays and immunohistochemistry were extensively studied in the 1980s to 1990s and ranged from 72.5% (7) to 95% (8) with frozen sections and from 67% (9) to 94% (10) with paraffin sections. Concordance rates between biochemical assays and immunohistochemistry were extensively studied in the 1980s to 1990s and ranged from 72.5% (7) to 95% (8) with frozen sections and from 67% (9) to 94% (10) with paraffin sections.…”

Expression of epidermal growth factor receptor (proto-oncogene c-erbB-1) and estrogen receptor in human breast carcinoma

“…Numerous studies have demonstrated the validity of the immunocytochemical methods to ascertain the levels of ER present in breast carcinoma (King et al, 1985;McCarty et al, 1985;Vlatkovic et al, 1987;Marchetti et al, 1987;Cudahy et al, 1988).…”

Ανοσοιστοχημικη Μελετη Της Κατανομης Των Γεννητικων Στεροειδων Ορμονων Και Των Ισοενζυμων Ββ Και Μμ Της Κρεατινοκινασης Σε Ογκους Των Σιελογονων Αδενων Ανθρωπου

“…Μία διαφορετική προσέγγιση του θέματος έγινε σε περιορισμένο αριθμό μελετών και αφορά την ανίχνευση των ενδογενών στεροειδών ορμονών, οιστραδιόλη και προγεστερόνη, σε νεοπλασματικά κύτταρα και κυρίως σε καρκινώματα του μαστού (Mercer et al, 1980;Taylor et al, 1981;Shimizu et al, 1983;Katayama et al, 1984;Pascal et al, 1986;Cudahy et al, 1988;0'Keane et al, 1990). Οι μέθοδοι που χρησιμοποιήθηκαν ήταν ο ανοσοφθορισμός και η ανοσοϊστοχημεία.…”