A comparison of the use of an ATP-based bioluminescent assay and image analysis for the assessment of bacterial adhesion to standard HEMA and biomimetic soft contact lenses

Andrews, Christopher S.; Denyer, Stephen Paul; Hall, Brenda; Hanlon, G. W.; Lloyd, Andrew W.

doi:10.1016/s0142-9612(01)00160-0

Cited by 34 publications

(14 citation statements)

References 17 publications

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“…Previous studies have elucidated that the initial bacterial adhesion to contact lenses increases with time, peaked at 3 to 18 hours of incubation and then remained steady, suggesting the end point of primary adhesion [22,31,37]. Bacterial adhesion during two phases of the process, two hours and 18 hours exposure of contact lenses to bacterial suspension were determined in this study.…”

Section: Resultsmentioning

confidence: 92%

“…Perhaps, the reduction in viability is due to the bacteria entering a biofilm mode of growth, which is known to result in lower viability of cells [39,40] or due to biofilm dispersal that can occur when the environment nutrients are not favorable for bacteria. In contrast, Stapleton et al [41] and Andrews et al [37] reported a plateau in adhesion that was reached after 45 minutes and four hours incubation respectively, with the adhesion that remained at those levels for more than 18 hours. These findings illustrates that investigators need to select incubation period of a bacterial adhesion carefully, depending upon study hypothesis being tested.…”

Section: Resultsmentioning

confidence: 96%

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A Laboratory Assessment of Factors That Affect Bacterial Adhesion to Contact Lenses

Dutta

Willcox

2013

Biology

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Adhesion of pathogenic microbes, particularly bacteria, to contact lenses is implicated in contact lens related microbial adverse events. Various in vitro conditions such as type of bacteria, the size of initial inoculum, contact lens material, nutritional content of media, and incubation period can influence bacterial adhesion to contact lenses and the current study investigated the effect of these conditions on bacterial adhesion to contact lenses. There was no significant difference in numbers of bacteria that adhered to hydrogel etafilcon A or silicone hydrogel senofilcon A contact lenses. Pseudomonas aeruginosa adhered in higher numbers compared to Staphylococcus aureus. Within a genera/species, adhesion of different bacterial strains did not differ appreciably. The size of initial inoculum, nutritional content of media, and incubation period played significant roles in bacterial adhesion to lenses. A set of in vitro assay conditions to help standardize adhesion between studies have been recommended.

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Section: Resultsmentioning

confidence: 92%

Section: Resultsmentioning

confidence: 96%

A Laboratory Assessment of Factors That Affect Bacterial Adhesion to Contact Lenses

Dutta

Willcox

2013

Biology

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“…Furthermore, in the current investigation, biofilms grew significantly in the first 48 h, and maturation and decelerated growth were not observed until then. In contrast, Stapleton et al [26] reported maximal adherence after 45 min, followed by a decrease in growth and Andrews et al [57] reported maximum adhesion following 4 h incubation. The results in the current study suggest that the conditions of the novel three-phase biofilm model may lead to slower growth over time, and the compounds of the artificial tear fluid may limit doubling times to rates more congruent with those expected in-vivo .…”

Section: Discussionmentioning

confidence: 98%

A three-phase in-vitro system for studying Pseudomonas aeruginosa adhesion and biofilm formation upon hydrogel contact lenses

et al. 2010

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BackgroundPseudomonas aeruginosa is commonly associated with contact lens (CL) -related eye infections, for which bacterial adhesion and biofilm formation upon hydrogel CLs is a specific risk factor. Whilst P. aeruginosa has been widely used as a model organism for initial biofilm formation on CLs, in-vitro models that closely reproduce in-vivo conditions have rarely been presented.ResultsIn the current investigation, a novel in-vitro biofilm model for studying the adherence of P. aeruginosa to hydrogel CLs was established. Nutritional and interfacial conditions similar to those in the eye of a CL wearer were created through the involvement of a solid:liquid and a solid:air interface, shear forces and a complex artificial tear fluid. Bioburdens varied depending on the CL material and biofilm maturation occurred after 72 h incubation. Whilst a range of biofilm morphologies were visualised including dispersed and adherent bacterial cells, aggregates and colonies embedded in extracellular polymer substances (EPS), EPS fibres, mushroom-like formations, and crystalline structures, a compact and heterogeneous biofilm morphology predominated on all CL materials.ConclusionsIn order to better understand the process of biofilm formation on CLs and to test the efficacy of CL care solutions, representative in-vitro biofilm models are required. Here, we present a three-phase biofilm model that simulates the environment in the eye of a CL wearer and thus generates biofilms which resemble those commonly observed in-situ.

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“…Staphylococcus epidermidis is a coagulase-negative staphylococcus (CNS) that often colonizes the skin and mucous membranes of the human body, representing an important part of its normal microflora [1, 2]. However, these staphylococci have emerged in the last years as the most frequently isolated pathogen in nosocomial sepsis, associated with implanted medical devices [3, 4], namely, prosthetic heart valves and joints, central venous catheters, urinary catheters, contact lenses, and hip prostheses [5].…”

Section: Introductionmentioning

confidence: 99%

Influence of Surface Properties on the Adhesion of Staphylococcus epidermidis to Acrylic and Silicone

Sousa

Teixeira

Oliveira

2009

International Journal of Biomaterials

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The aim of the present study was to compare the ability of eight Staphylococcus epidermidis strains to adhere to acrylic and silicone, two polymers normally used in medical devices manufacture. Furthermore, it was tried to correlate that with the surface properties of substrata and cells. Therefore, hydrophobicity and surface tension components were calculated through contact angle measurements. Surface roughness of substrata was also assessed by atomic force microscopy (AFM). No relationship was found between microbial surface hydrophobicity and adhesion capability. Nevertheless, Staphylococcus epidermidis IE214 showed very unique adhesion behaviour, with cells highly aggregated between them, which is a consequence of their specific surface features. All strains, determined as being hydrophilic, adhered at a higher extent to silicone than to acrylic, most likely due to its more hydrophobic character and higher roughness. This demonstrates the importance of biomaterial surface characteristics for bacterial adhesion.

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A comparison of the use of an ATP-based bioluminescent assay and image analysis for the assessment of bacterial adhesion to standard HEMA and biomimetic soft contact lenses

Cited by 34 publications

References 17 publications

A Laboratory Assessment of Factors That Affect Bacterial Adhesion to Contact Lenses

A Laboratory Assessment of Factors That Affect Bacterial Adhesion to Contact Lenses

A three-phase in-vitro system for studying Pseudomonas aeruginosa adhesion and biofilm formation upon hydrogel contact lenses

Influence of Surface Properties on the Adhesion of Staphylococcus epidermidis to Acrylic and Silicone

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