A Comparison of the Transport of Spermatozoa in Spayed and Entire Ewes

Allison, A. J.

doi:10.1530/jrf.0.0310415

Cited by 7 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Spermatozoa were recovered by the methods described by Allison (1972) and Lightfoot & Salamon (1970) and counted by the methods of Mattner & Braden (1963) and Allison & Robinson (1970). Each cervix was cut, while frozen, into caudal, mid and cranial thirds and each third was divided into 6-8 longitudinal strips.…”

Section: Experimen Tal Proceduresmentioning

confidence: 99%

Plasma progesterone concentrations, ovarian and endocrinological responses and sperm transport in ewes with synchronized oestrus

Pearce¹,

Robinson²

1985

Reproduction

View full text Add to dashboard Cite

Two experiments involving 24 and 54 Australian Merino ewes were conducted in which the establishment of a cervical population of spermatozoa and several endocrinological events were studied after several regimens for the synchronization of oestrus. Intravaginal sponges impregnated with 500 mg (Exp. 1) or 200, 400 or 600 mg (Exp. 2) progesterone resulted in the maintenance of plasma progesterone concentrations of 1.5-4.9 ng/ml over a 12-day insertion period compared with 1.9-6.9 ng/ml during dioestrus in control ewes. In Exp. 1 basal concentrations of less than or equal to 0.25 ng/ml plasma were attained by 4 h after sponge withdrawal and this decline was much more rapid than in normal luteolysis. This was associated with fewer spermatozoa recovered from the cervix 2 h after insemination, and PMSG had no significant effect. In Exp. 2 injection of a supplementary dose of progesterone at sponge withdrawal resulted in a rapid increase in plasma progesterone concentrations followed by an equally rapid decrease and an attenuation of the rise in plasma oestradiol-17 beta, the LH surge, and the onset of oestrus. The numbers of spermatozoa recovered 4 h after insemination were not increased, and PMSG had no significant effect. Two factors were significant, namely the dose of progesterone in the sponge (600 mg greater than 400 or 200 mg, P less than 0.05) and stage of oestrus when inseminated (mid- or late oestrus greater than early). The data demonstrated that an adequate dose of progesterone/progestagen incorporated into intravaginal sponges and accurate timing of insemination relative to the LH surge are the most important factors involved in penetration of the cervix by spermatozoa.

show abstract

Section: Experimen Tal Proceduresmentioning

confidence: 99%

Plasma progesterone concentrations, ovarian and endocrinological responses and sperm transport in ewes with synchronized oestrus

Pearce¹,

Robinson²

1985

Reproduction

View full text Add to dashboard Cite

show abstract

Influence of the uterine environment on rat sperm motility and swimming speed

Goeden

Zenick

1985

J. Exp. Zool.

View full text Add to dashboard Cite

The present study compared several rat sperm parameters in semen samples recovered from a natural uterine environment (i.e., intact estrous female) to those recovered from an artificially induced uterine environment (i.e., ovariectomized hormonally primed female). The sperm parameters measured were percent motile, percent exhibiting forward progressive motility, actual swimming speed, and linear swimming speed. The comparisons were conducted at four postcopulatory time points (0.25, 1.5, 3, and 6 hours) in order to detect differences as a function of residence time within the uterus. No significant differences (P less than 0.05) in the parameters were seen between the two types of uterine environments. Residence time within the reproductive tract had no significant effect on the parameters with the exception of percent motile, which was significantly increased (P less than 0.01) at the 1.5-hour postcopulatory time point.

show abstract