A comparison of the temperature dependence of charge recombination in the ion-radical pair P870+QA - and tryptophan fluorescence in the photosynthetic reaction centers of Rhodobacter sphaeroides

The temperature dependences of tryptophan fluorescence decay kinetics in aqueous glycerol and 1 M trehalose solutions were examined. The fluorescence decay kinetics were recorded in the spectral region of 292.5-417.5 nm with nanosecond time resolution. The kinetics curves were approximated by the sum of three exponential terms, and the spectral distribution (DAS) of these components was determined. An antisymbatic course of fluorescence decay times of two (fast and medium) components in the temperature range from -60 to +10°C was observed. The third (slow) component showed only slight temperature dependence. The antisymbatic behavior of fluorescence lifetimes of the fast and medium components was explained on the assumption that some of the excited tryptophan molecules are transferred from a short-wavelength B-form with short fluorescence lifetime to a long-wavelength R-form with an intermediate fluorescence lifetime. This transfer occurred in the indicated temperature range.

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A comparison of the temperature dependence of charge recombination in the ion-radical pair P870+QA - and tryptophan fluorescence in the photosynthetic reaction centers of Rhodobacter sphaeroides

Cited by 2 publications

References 32 publications

Comparison of tryptophan fluorescence lifetimes in cyanobacterial photosystem I frozen in the light and in the dark

Comparison of tryptophan fluorescence lifetimes in cyanobacterial photosystem I frozen in the light and in the dark

Temperature dependence of tryptophan fluorescence lifetime in aqueous glycerol and trehalose solutions

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