A comparison of the serum proteins of normal rats with those of rats bearing liver tumours

Campbell, P. N.; Kernot, Barbara A.; Roitt, Ivan M.

doi:10.1042/bj0710155

Cited by 19 publications

(7 citation statements)

References 14 publications

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“…Darcy found that the ac-globulin was also present in the serum of rats with actively growing tissue, (regenerating liver, kidney or skin) and in pregnant females. These finds were confirmed by Campbell, Kernot and Roitt (1959). In clinical studies employing physico-chemical methods non-specific changes in mucoproteins in neoplastic infections and other conditions have been described (Winzler, 1953;Lockey, Anderson and Maclagan, 1956) and it may be possible that something of the same sort occurred here.…”

Section: Discussionsupporting

confidence: 70%

Anaphylactic tests for tumour antigen in transplantable animal tumours.

Dale¹

1966

Br J Cancer

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TECHNIQUES based on the anaphylactic response have been used in many investigations into possible differences between normal and neoplastic tissues. Maver and Barret (1943) used systemic anaphylaxis along with precipitin and complement fixation techniques in an attempt to distinguish between cathepsins of normal and neoplastic tissues. Zilber (1958) and his co-workers employed anaphylaxis extensively as an experimental tool. They reported differences betweeni normal and malignant tissue in rats, mice and rabbits and inferred that the method demonstrated the existence of tumour-specific antigens. Similar studies were done on rabbit tumours bv Artamonova (1959), on various human tumours by Gorodilova and Shershul'skaia (1959), on various tumours of rats and mice by Levina (1959), on experimental mouse hepatomata by Korosteleva (1959) and on the Guerin rat epithelioma by Ryzewska (1962).Makari (1955) It is evident that if Makari's claim were valid, this would be a test of considerable clinical importance. Even if the test gave positive results in a limited number of cases, as reported by Hackett and Gardonyi, it might still be a useful investigatory tool in tumour research. Accordingly it was decided to undertake an evaluation of the applicability of anaphylactic tests to this type of study. Two tests were investigated: the Dale-Schultz reaction and the measurement of histamine-release from chopped lung. In the first part of the project (Dale, 1965a and b) it was shown that some of the assumptions on which the use of the tests was based were invalid. Certain general procedural limits were outlined within which the tests might possibly be used to analyse antigen mixtures. Using these limits a mock tumour antigen study was then carried out to determine whether.if a cancer antigen were present in tissue and serum, it could be detected by the tests (Dale, 1965c). From this latter study it was concluded that the tests might possibly give positive results if a cancer antigen were either highly antigenic or else were present in fairly high concentration in both tumour extract and serum. In the present study two questions were posed

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Section: Discussionsupporting

confidence: 70%

Anaphylactic tests for tumour antigen in transplantable animal tumours.

Dale¹

1966

Br J Cancer

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“…When the activity of the microsome fraction from different tissues is compared the correlation is rather poor. For example, although the microsome fraction from guinea-pig liver seems to be as active as that from rat liver, among the birds we found that similar fractions from the livers of pigeon, turkey and chicken were inactive, whereas that from young ducks was nearly as active as that from rat liver (Campbell & Kernot, 1959~). There is obviously an interesting field here for a comparison between morphological structure and protein synthetic activity.…”

Section: Comparative Activity Of the Microsome Fractions From Vamentioning

confidence: 75%

The Synthesis of Proteins by the Cytoplasmic Components of Animal Cells*

Campbell

1960

Biological Reviews

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“…(Campbell et al 1960;Campbell & Kernot, 1962) that the microsomal fraction of rat liver can synthesize rat serum albumin. In these studies the synthesis of a heterologous albumin was investigated by using microsomes from rat liver and cell sap from duck liver (Campbell & Kernot, 1959). Although the incorporation of 14C-labelled amino acids into proteins in general and into rat serum albumin in particular was high, there was no synthesis of duck serum albumin by the rat-liver microsomes.…”

Section: Estimation Of Radioactivity Of Immunologicalmentioning

confidence: 99%

Labelling of Immunologically Specific Proteins by Ribonucleoprotein Particles From Rat-Liver and Chick-Liver Cell Sap

Deckena¹

1963

Biochemical Journal

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A comparison of the serum proteins of normal rats with those of rats bearing liver tumours

Cited by 19 publications

References 14 publications

Anaphylactic tests for tumour antigen in transplantable animal tumours.

Anaphylactic tests for tumour antigen in transplantable animal tumours.

The Synthesis of Proteins by the Cytoplasmic Components of Animal Cells*

Labelling of Immunologically Specific Proteins by Ribonucleoprotein Particles From Rat-Liver and Chick-Liver Cell Sap

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