A Comparison of the Properties of Klebsiella Strains Isolated From Different Sources

An investigation into the properties of klebsiella strains isolated from ankylosing spondylitis patients

“…The results of the tests described in this paper indicate that whilst the isolates are all definitely identifiable as klebsiellas, there is no obvious way in which they differ from those found in hospital and other environments (Cooke et al 1979a(Cooke et al , b, 1980Edmondson et al 1980). All these strains fell into the five groups described by Edmondson et al (1980) except one which was positive in the indole test and negative in the other 3.…”

“…the faecal coliform test, the production of acid and gas in bile-salt broth at 44-5°C, growth in nutrient broth at 10°C, indole production and pectin liquefaction. The results of these tests are shown in Table 1, and with one exception (the strain which was positive in the indole tests and negative in the other 3) they all fell into one of the five groups described by Edmondson et al (1980) 21 (1) …”

“…indole production, dulcitol fermentation, methyl red test, Voges The organisms were also examined with the four tests used by Edmondson et al (1980), i.e. the faecal coliform test, the production of acid and gas in bile-salt broth at 44-5°C, growth in nutrient broth at 10°C, indole production and pectin liquefaction.…”

“…Where more than one method is described the following were chosen: carbohydrate test method 1; catalase 1; oxidase 1; arginine, ornithine and lysine decarboxylase 2; urease 1; Voges Proskauer 2; gelatin hydrolysis 1 and 5; malonate utilization 1; indole production 2; gluconate oxidation 1; H2S production 1. Two further tests, namely growth at 10 'C and pectin liquefaction were performed according to the methods of Edmondson et al (1980) with some modification. For growth at 10 'C, one loopful of a broth culture was used as the inoculum, a stirred water-bath was used and the results were read visually.…”

“…Another group of workers has demonstrated that antisera raised against certain strains of K. pneumoniae are cytotoxic for B27 positive lymphocytes from AS patients, but not for B27 positive controls (Seager et al 1979;Geczy & Yap, 1979;Geezy, Alexander & Bashir, 1980). The purpose of this paper is to describe and identify the klebsiellas isolated by one of us (D. C.) from AS patients, in order to see if there is any detectable distinguishing feature about them, as opposed to those found in non AS patients and other environments (Cooke et al 1979a(Cooke et al , b, 1980Edmondson et al 1980). …”