A comparison of the promoter strengths of two eukaryotic genes<i>in vitro</i>reveals a region of DNA that can influence the rate of transcription in cis over long distances

Gregory, Stephen P.; Butterworth, Peter H. W.

doi:10.1093/nar/11.16.5317

Cited by 8 publications

(1 citation statement)

References 22 publications

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“…The sequences located between 400 and -1400 bp upstream of the mouse (3-major globin genes have been shown to have a repressive effect on transcription from the promoter immediately downstream (Gregory and Butterworth, 1983;Gilmour et al, 1984). Contained within this 1-kb repressing region is 200-bp alternating purine pyrimidine sequence.…”

Section: Discussionmentioning

confidence: 99%

(AT)n is an interspersed repeat in the Xenopus genome.

Greaves¹,

Patient²

1985

The EMBO Journal

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We have observed (AT)34 and (AT)23 tracts close to the coding sequences of the Xenopus laevis tadpole alpha T1 and adult beta 1 globin genes, respectively. We show that (AT)n sequences are found as interspersed repeats within the Xenopus globin and histone gene loci. Using (AT)n co‐polymer in filter hybridisation experiments we estimate that there are 10(4) (AT)n tracts per haploid Xenopus genome. Hybridisation to genomic blots of DNA from yeast, slime mold, trypanosome, fruit fly, salmon, chicken, rat, human, crab and Xenopus species shows that strictly alternating AT of sufficient length to hybridise appears to be most abundant in Xenopus and crab genomes. We show that the specificity of the co‐polymer probe for strictly alternating AT is, however, dependent on the length of the probe. Hybridisation experiments using (TG)n copolymer suggest that this highly conserved repeat is found as clustered repeats in the Xenopus genome in contrast to other eukaryotic genomes so far studied.

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Section: Discussionmentioning

confidence: 99%

(AT)n is an interspersed repeat in the Xenopus genome.

Greaves¹,

Patient²

1985

The EMBO Journal

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A negative regulatory sequence near the mouse beta-maj globin gene associated with a region of potential Z-DNA.

Gilmour¹,

Spandidos²,

Vass³

et al. 1984

The EMBO Journal

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The possible regulatory role of DNA sequences situated 5′ to the beta‐maj globin gene was investigated by two types of assay. First, a long term transformation assay was used to measure the efficiency of transformation of TK‐ mouse (LATK‐) and hamster (BHKTK‐) fibroblast cells with DNA molecules made by covalently linking mouse and human DNA fragments to the herpes simplex virus (HSV‐1) thymidine kinase (tk) gene in the plasmid pTK1. When the promoter regions from the mouse beta‐maj globin or the human epsilon‐globin genes are substituted for the viral promoter in the tk gene transformation occurs with 10‐20% of the efficiency of the original plasmid. A fragment (H1), containing sequences between 344 and 1413 bp upstream from the mouse beta‐maj globin cap site, almost completely abolishes transformation when inserted next to hybrid tk genes containing the mouse beta‐globin or human epsilon‐globin promoter but has no effect on the intact tk gene. The effect can be demonstrated with the H1 fragment in either orientation relative to the tk gene. Secondly, in transient expression assays the H1 fragment strongly inhibits transcription when covalently linked to the tk gene under control of either globin promoter, but not when linked to the tk gene with its own promoter. The H1 fragment contains 53 bp of purine‐pyrimidine alternation (ACAT)n as part of a larger region potentially capable of adopting a Z‐DNA conformation.

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Chromatin Structure and Gene Expression

Conklin

Groudine

1984

DNA Methylation

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A comparison of the promoter strengths of two eukaryotic genesin vitroreveals a region of DNA that can influence the rate of transcription in cis over long distances

Cited by 8 publications

References 22 publications

(AT)n is an interspersed repeat in the Xenopus genome.

(AT)n is an interspersed repeat in the Xenopus genome.

A negative regulatory sequence near the mouse beta-maj globin gene associated with a region of potential Z-DNA.

Chromatin Structure and Gene Expression

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