A comparison of the pro-angiogenic potential of human induced pluripotent stem cell derived endothelial cells and induced endothelial cells in a murine model of peripheral arterial disease

Clayton, Zoe; Yuen, Gloria; Sadeghipour, Sara; Hywood, Jack D.; Wong, J.; Huang, Ngan F.; Ng, M.; Cooke, John P.; Patel, Sanjay

doi:10.1016/j.ijcard.2017.01.125

Cited by 34 publications

(43 citation statements)

References 45 publications

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“…hiPSC-EC also improved blood flow to ischemic limbs by increasing the total number of capillaries as well as angiogenic cytokines and growth factors [63]. Compared to induced endothelial cells generated from fibroblasts, hiPSC-EC show similar perfusion recovery, although capillary density in the ischemic muscle was only increased in the induced endothelial cell group [64]. Furthermore, hiPSC-EC enhanced angiogenesis via paracrine signaling in ischemic tissue [65].…”

Section: Human-induced Pluripotent Stem Cells In Ischemic Woundsmentioning

confidence: 99%

“…The majority of studies employing iPSC in models of peripheral arterial disease have delivered cells or extracellular vesicles via intramuscular (IM) injection with cells suspended in buffer or culture medium [61,[63][64][65][66][67][68][69]. Cell survival was increased when the cells were suspended in a recombinant hydrogel, which may prevent cell membrane damage from the shear stress of injection [70].…”

Section: Human-induced Pluripotent Stem Cells In Ischemic Woundsmentioning

confidence: 99%

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The potential and limitations of induced pluripotent stem cells to achieve wound healing

et al. 2019

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Wound healing is the physiologic response to a disruption in normal skin architecture and requires both spatial and temporal coordination of multiple cell types and cytokines. This complex process is prone to dysregulation secondary to local and systemic factors such as ischemia and diabetes that frequently lead to chronic wounds. Chronic wounds such as diabetic foot ulcers are epidemic with great cost to the healthcare system as they heal poorly and recur frequently, creating an urgent need for new and advanced therapies. Stem cell therapy is emerging as a potential treatment for chronic wounds, and adult-derived stem cells are currently employed in several commercially available products; however, stem cell therapy is limited by the need for invasive harvesting techniques, immunogenicity, and limited cell survival in vivo. Induced pluripotent stem cells (iPSC) are an exciting cell type with enhanced therapeutic and translational potential. iPSC are derived from adult cells by in vitro induction of pluripotency, obviating the ethical dilemmas surrounding the use of embryonic stem cells; they are harvested non-invasively and can be transplanted autologously, reducing immune rejection; and iPSC are the only cell type capable of being differentiated into all of the cell types in healthy skin. This review focuses on the use of iPSC in animal models of wound healing including limb ischemia, as well as their limitations and methods aimed at improving iPSC safety profile in an effort to hasten translation to human studies.

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Section: Human-induced Pluripotent Stem Cells In Ischemic Woundsmentioning

confidence: 99%

Section: Human-induced Pluripotent Stem Cells In Ischemic Woundsmentioning

confidence: 99%

The potential and limitations of induced pluripotent stem cells to achieve wound healing

et al. 2019

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“…Numerous studies have been explored in animal models where preclinical studies have established the safety, feasibility, and efficacy of stem cell-based therapy in CLI [ 50 , 51 ]. In a murine model simulating PAD through surgical hindlimb ischemia, iPS cells generated from human fibroblasts were intramuscularly injected postoperatively with perfusion recovery measured by laser Doppler [ 52 ]. Significantly improved reperfusion and greater capillary density in the ischemic gastrocnemius muscle was demonstrated in treated mice as compared to control by postoperative day 14 [ 52 ].…”

Section: Preclinical Testing Of Stem Cell Therapymentioning

confidence: 99%

“…In a murine model simulating PAD through surgical hindlimb ischemia, iPS cells generated from human fibroblasts were intramuscularly injected postoperatively with perfusion recovery measured by laser Doppler [ 52 ]. Significantly improved reperfusion and greater capillary density in the ischemic gastrocnemius muscle was demonstrated in treated mice as compared to control by postoperative day 14 [ 52 ]. Furthermore, on histology, there was significantly less myofiber heterogeneity, nuclear centralization, fatty degeneration, and fibrosis in iPS cell-treated hindlimbs as compared to controls, indicating less tissue damage [ 52 , 53 ].…”

Section: Preclinical Testing Of Stem Cell Therapymentioning

confidence: 99%

“…Significantly improved reperfusion and greater capillary density in the ischemic gastrocnemius muscle was demonstrated in treated mice as compared to control by postoperative day 14 [ 52 ]. Furthermore, on histology, there was significantly less myofiber heterogeneity, nuclear centralization, fatty degeneration, and fibrosis in iPS cell-treated hindlimbs as compared to controls, indicating less tissue damage [ 52 , 53 ]. Similarly, transplantation of allogeneic adipose-derived regenerative cells has been shown to be a promising treatment modality for severe ischemic diseases [ 54 ].…”

Section: Preclinical Testing Of Stem Cell Therapymentioning

confidence: 99%

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A Molecular and Clinical Review of Stem Cell Therapy in Critical Limb Ischemia

Parikh

Liu

Velázquez

2017

Stem Cells International

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Peripheral artery disease (PAD) is one of the major vascular complications in individuals suffering from diabetes and in the elderly that can progress to critical limb ischemia (CLI), portending significant burden in terms of patient morbidity and mortality. Over the last two decades, stem cell therapy (SCT) has risen as an attractive alternative to traditional surgical and/or endovascular revascularization to treat this disorder. The primary benefit of SCT is to induce therapeutic neovascularization and promote collateral vessel formation to increase blood flow in the ischemic limb and soft tissue. Existing evidence provides a solid rationale for ongoing in-depth studies aimed at advancing current SCT that may change the way PAD/CLI patients are treated.

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Skin wound healing assisted by angiogenic targeted tissue engineering: A comprehensive review of bioengineered approaches

Nour

Imani

Chaudhry

et al. 2020

J Biomedical Materials Res

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Skin injuries and in particular, chronic wounds, are one of the major prevalent medical problems, worldwide. Due to the pivotal role of angiogenesis in tissue regeneration, impaired angiogenesis can cause several complications during the wound healing process and skin regeneration. Therefore, induction or promotion of angiogenesis can be considered as a promising approach to accelerate wound healing. This article presents a comprehensive overview of current and emerging angiogenesis induction methods applied in several studies for skin regeneration, which are classified into the cell, growth factor, scaffold, and biological/chemical compound‐based strategies. In addition, the advantages and disadvantages of these angiogenic strategies along with related research examples are discussed in order to demonstrate their potential in the treatment of wounds.

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A comparison of the pro-angiogenic potential of human induced pluripotent stem cell derived endothelial cells and induced endothelial cells in a murine model of peripheral arterial disease

Cited by 34 publications

References 45 publications

The potential and limitations of induced pluripotent stem cells to achieve wound healing

The potential and limitations of induced pluripotent stem cells to achieve wound healing

A Molecular and Clinical Review of Stem Cell Therapy in Critical Limb Ischemia

Skin wound healing assisted by angiogenic targeted tissue engineering: A comprehensive review of bioengineered approaches

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