A comparison of the presence of Theileria ovis by PCR amplification of their SSU rRNA gene in small ruminants from two provinces of Pakistan

Durrani, Sobia; Khan, Zaheer; Khattak, Rehman Mehmood; Andleeb, Mehwish; Ali, Muhammad Arif; Hameed, Hafsa; Taqddas, Asia; Faryal, M.; Kiran, Shumaila; Anwar, Humera; Riaz, Muhammad; Sajid, Muhammad Sohail; Sheikh, Rehan Sadiq; Iqbal, Furhan

doi:10.1016/s2222-1808(12)60010-3

Cited by 25 publications

(19 citation statements)

References 20 publications

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“…This indicator has little value because this study was trans-sectional and carried out on healthy animals. Other findings reported the presence of a positive correlation between tick burdens and infection prevalence (Aktas et al 2005 ; Durrani et al 2012 ; Iqbal et al 2013 ).…”

Section: Discussionmentioning

confidence: 89%

Prevalence of Theileria and Babesia species in Tunisian sheep

Rjeibi

Darghouth

Gharbi

2016

Onderstepoort J Vet Res

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In this study, the prevalence of Theileria and Babesia species in sheep was assessed with Giemsa-stained blood smear examination and polymerase chain reaction to identify the different piroplasms in 270 sheep from three Tunisian bioclimatic zones (north, centre, and south). The overall infection prevalence by Babesia spp. and Theileria spp. in Giemsa-stained blood smears was 2.9% (8/270) and 4.8% (13/270) respectively. The molecular results showed that sheep were more often infected by Theileria ovis than Babesia ovis with an overall prevalence of 16.3% (44/270) and 7.8% (21/270) respectively (p = 0.01). The molecular prevalence by Babesia ovis was significantly higher in females than in males (p < 0.05). According to localities B. ovis was found exclusively in sheep from the centre of Tunisia (Kairouan) whereas Theileria ovis was found in all regions. Infections with T. ovis and B. ovis were confirmed by sequencing. The sequence of T. ovis in this study (accession numbers KM924442) falls into the same clade as T. ovis deposited in GenBank. The T. ovis amplicons (KM924442) showed 99%–100% identities with GenBank sequences. Moreover, comparison of the partial sequences of 18S rRNA gene of B. ovis described in this study (KP670199) revealed 99.4% similarity with B. ovis recently reported in northern Tunisia from sheep and goats. Three nucleotides were different at positions 73 (A/T), 417 (A/T), and 420 (G/T). It also had 99% identity with B. ovis from Spain, Turkey and Iraq. The results suggest a high T. ovis prevalence in Tunisia with a decreasing north-south gradient. This could be correlated to the vector tick distribution.

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Section: Discussionmentioning

confidence: 89%

Prevalence of Theileria and Babesia species in Tunisian sheep

Rjeibi

Darghouth

Gharbi

2016

Onderstepoort J Vet Res

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“…The study revealed an overall prevalence of 45.1% for both pathogens in small ruminants from northern Pakistan of which 23.3% were Theileria , which is consistent with previous studies (17.4–31.2%) from Bannu, Tank and Dera Ismail Khan districts in southern Khyber Pakhtunkhwa [ 58 ] and elsewhere (17.8 and 17.2% in Turkey and China, respectively) [ 8 , 64 ]. However, some studies reported contrasting prevalences; for example, a study by Nasreen et al [ 38 ] in the Dir District, Lower KP showed a prevalence of 53.5%, while Durrani et al [ 13 ] reported a prevalence of 35% from Lahore with PCR. Similarly, a lower prevalence (3.47%) was reported in Southern Punjab [ 17 ], 6% in Kohat and Peshawar [ 13 ], while Saeed et al [ 50 ] reported a prevalence of 3% in Kohat and Peshawar.…”

Section: Discussionmentioning

confidence: 99%

“…However, some studies reported contrasting prevalences; for example, a study by Nasreen et al [ 38 ] in the Dir District, Lower KP showed a prevalence of 53.5%, while Durrani et al [ 13 ] reported a prevalence of 35% from Lahore with PCR. Similarly, a lower prevalence (3.47%) was reported in Southern Punjab [ 17 ], 6% in Kohat and Peshawar [ 13 ], while Saeed et al [ 50 ] reported a prevalence of 3% in Kohat and Peshawar. These differences may be due to different geographical areas and climatic conditions [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

“…It is estimated that livestock production is the source of 35–40% of income for over 8 million rural families in Pakistan [ 41 ]. The Punjab and Sindh provinces of Pakistan have suitable land for crops and have more developed industrial sectors, but the population of Khyber Pakhtunkhwa (KP) province is highly dependent on livestock production and it significant contributes to livelihoods [ 13 , 39 ]. Successful livestock production is hampered by a variety of factors such as mismanagement, infestation by ectoparasites, contagious infectious diseases, and nutritional deficiencies, preventing KP from fulfilling the meat, milk and hide requirements for an increasing human population [ 58 ].…”

Section: Introductionmentioning

confidence: 99%

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Molecular prevalence, characterization and associated risk factors of Anaplasma spp. and Theileria spp. in small ruminants in Northern Pakistan

et al. 2021

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This study was conducted in four districts (Malakand, Swat, Bajaur and Shangla) of Northern Pakistan to investigate the prevalence, associated risk factors and phylogenetic analyses of Theileria and Anaplasma species in small ruminants. A total of 800 blood samples, 200 from each district, were collected from apparently healthy animals. PCR assays were performed using generic primers for Anaplasma spp. and Theileria spp. as well as species specific primers for A. ovis and T. ovis. Overall infection prevalence was 361/800 (45.1%). Theileria spp. infection prevalence (187/800, 23.3%) was higher than Anaplasma spp. (174/800, 21.7%). Amplified partial 18S rRNA genes were sequenced and enrolled animals were found to be infected by T. ovis (115/800, 14.3%), and at least two more Theileria species (72/800, 9%) were present (T. lestoquardi and T. annulata). All blood samples that were found to be positive for Anaplasma spp. were also positive for A. ovis. Infection prevalence was higher in sheep (227/361, 28.3%) compared to goats (134/361, 16.6%) (p < 0.005). Univariable analysis of risk factors showed that host, age, grazing system and acaricide treatment were significant determinants (p < 0.05) for both Theileria and Anaplasma infections. Multivariable analysis revealed that host, sex, age, tick infestation and grazing system were significant risk factors (p < 0.005) for both pathogens. Phylogenetic analysis revealed variants among the A. ovis and T. annulata samples analysed, indicating that different genotypes are circulating in the field while T. ovis presented the same genotype for the samples analysed.

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“…DNA concentration was determined by NanoDrop Spectrophotometer. Theileria ovis specific primers given as follows were used (Durrani et al, 2012): TSsr170F (5'-TCGAGACCTTCGGGT-3') TSsr 670R MAŚLANKA T., ZUŚKA-PROT M.…”

Section: Methodsmentioning

confidence: 99%

Comparative pathological changes in sheep infected with Theileria annulata and non-infected control

Akhter

Aslam

Rehman

et al. 2018

J Hellenic Vet Med Soc

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The hematological, serum biochemical and histopathological variations were compared in sheep naturally infected with Theileria annulata and healthy control group. Peripheral blood smears of 300 suspected sheep were observed for the presence of Theileria by microscopy (24%) and confirmed through PCR (34%). The PCR confirmed samples were used for further studies and showed significant decrease in hemoglobin concentration, packed cell volume (PCV), total erythrocyte counts, total leukocyte count, serum total proteins, creatinine and glucose (P < 0.05) as compared to healthy control. Similarly a significant increase was recorded in Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) (P< 0.05) as compared to non-infected sheep. Histopathological changes revealed edema and severe depletion of lymphocytes in lymph nodes. The present study concluded that ovine theileriosis was linked with some pathological alterations in blood and tissues which could be helpful in the diagnosis of disease.

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A comparison of the presence of Theileria ovis by PCR amplification of their SSU rRNA gene in small ruminants from two provinces of Pakistan

Cited by 25 publications

References 20 publications

Prevalence of <i>Theileria</i> and <i>Babesia</i> species in Tunisian sheep

Prevalence of <i>Theileria</i> and <i>Babesia</i> species in Tunisian sheep

Molecular prevalence, characterization and associated risk factors of Anaplasma spp. and Theileria spp. in small ruminants in Northern Pakistan

Comparative pathological changes in sheep infected with Theileria annulata and non-infected control

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