A comparison of the mutagenic effectiveness of chronic neutron- and γ-irradiation of mouse spermatogonia

Batchelor, A.L.; Phillips, Rita J. S.; Searle, A.G.

doi:10.1016/0027-5107(66)90063-7

Cited by 58 publications

(11 citation statements)

References 13 publications

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“…In radiation mutagenesis, however, the frequency of X-ray-induced viable mutants in spermatogonia was only 23% (Russell and Russell 1959), suggesting that mutations induced by X-rays in spermatogonia might include some chromosomal abnormalities. Similar events were also observed in experiments using gamma-rays and fission neutrons (Batchelor et al 1966). The frequency of homozygous Table 2.…”

Section: Resultssupporting

confidence: 76%

Genetic analysis of mutants in mice induced by N-ethyl-N-nitrosourea.

Murota

Shibuya

1983

The Japanese journal of genetics

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Specific locus tests of the effects of N-ethyl-N-nitrosourea (ENU) on mouse spermatogonia using visible loci and hemoglobin beta-chain (Hbb) locus have been performed.Male wild-type (C3Hf/He) mice were injected intraperitoneally with ENU. Eight weeks after injection, the males were mated with tester strain females (PW) homozygous for six visible recessive genes. Ten visible and 2 hemoglobin mutants were obtained.The mutation frequency was 60.3 x 10-5/locus/generation. These mutations were all heritable, and nine out of ten mutations were viable under homozygous conditions. The results suggest that the mutations induced by ENU on spermatogonia of mice might have resulted from events at the genic rather than the chromosomal level.

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Section: Resultssupporting

confidence: 76%

Genetic analysis of mutants in mice induced by N-ethyl-N-nitrosourea.

Murota

Shibuya

1983

The Japanese journal of genetics

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“…The Rw mutation was recovered in the course of radiation mutagenesis experiments, identifiable by the dominant pigmentation phenotype, and also noted to be recessive lethal in the initial report (Batchelor et al 1966). Since the lethality appears not to be due to disruption of sequences at the inversion breakpoints, it may be caused by an intragenic mutation, or small deletion, that arose concurrently from radiation treatment.…”

Section: Rw Lethality Is Not Due To Disruption Of Dpp6mentioning

confidence: 96%

Random mutagenesis of proximal mouse chromosome 5 uncovers predominantly embryonic lethal mutations

Wilson

Ching²,

Farias³

et al. 2005

Genome Res.

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A region-specific ENU mutagenesis screen was conducted to elucidate the functional content of proximal mouse Chr 5. We used the visibly marked, recessive, lethal inversion Rump White (Rw) as a balancer in a three-generation breeding scheme to identify recessive mutations within the ∼50 megabases spanned by Rw. A total of 1003 pedigrees were produced, representing the largest inversion screen performed in mice. Test-class animals, homozygous for the ENU-mutagenized proximal Chr 5 and visibly distinguishable from nonhomozygous littermates, were screened for fertility, hearing, vestibular function, DNA repair, behavior, and dysmorphology. Lethals were identifiable by failure to derive test-class animals within a pedigree. Embryonic lethal mutations (total of 34) were overwhelmingly the largest class of mutants recovered. We characterized them with respect to the time of embryonic death, revealing that most act at midgestation (8.5-10.5) or sooner. To position the mutations within the Rw region and to guide allelism tests, we performed complementation analyses with a set of new and existing chromosomal deletions, as well as standard recombinational mapping on a subset of the mutations. By pooling the data from this and other region-specific mutagenesis projects, we calculate that the mouse genome contains ∼3479-4825 embryonic lethal genes, or about 13.7%-19% of all genes.

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“…In mice, the RBE value for specific-locus mutations has been estimated to be about 6 in both sperm and spermatogonia (Russell and Kelley 1964;Russell 1965) and the value increases up to 23 or more under chronic irradiations in spermatogonia (Batchelor et al 1966;Searle and Phillips 1964a). By way of precaution, it should be mentioned that there is a consistent picture of RBE values approximately 6 for both dominant lethal and specific-locus mutations in mice post-spermatogonial cells (Russell and Kelley 1964;Russell 1965).…”

Section: Discussionmentioning

confidence: 99%

RELATIVE BIOLOGICAL EFFECTIVENESS OF 14MeV NEUTRONS FOR INDUCING DOMINANT LETHALS IN MATURE SPERM OF THE SILKWORM: A COMPARISON OF THE RBE FOR DOMINANT LETHALS AND SPECIFIC-LOCUS MUTATIONS

Murakami

1971

The Japanese journal of genetics

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A comparison of the mutagenic effectiveness of chronic neutron- and γ-irradiation of mouse spermatogonia

Cited by 58 publications

References 13 publications

Genetic analysis of mutants in mice induced by N-ethyl-N-nitrosourea.

Genetic analysis of mutants in mice induced by N-ethyl-N-nitrosourea.

Random mutagenesis of proximal mouse chromosome 5 uncovers predominantly embryonic lethal mutations

RELATIVE BIOLOGICAL EFFECTIVENESS OF 14MeV NEUTRONS FOR INDUCING DOMINANT LETHALS IN MATURE SPERM OF THE SILKWORM: A COMPARISON OF THE RBE FOR DOMINANT LETHALS AND SPECIFIC-LOCUS MUTATIONS

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