A comparison of the interferon gamma assay with the absorbed ELISA for the diagnosis of Johne's disease in cattle

Billman‐Jacobe, Helen; Carrigan, M. J.; Cockram, F. A.; Corner, L. A.; Gill, I J; Hill, John; Jessep, T.; Milner, Anthony; Wood, P.R.

doi:10.1111/j.1751-0813.1992.tb07426.x

Cited by 75 publications

(34 citation statements)

References 11 publications

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“…The observation in this study of an early IFN-␥ response followed later by antibody and LT responses is comparable to previously published data on the kinetics of immune reactivity to M. avium subsp. paratuberculosis infections in cattle and goats (5,40,42,44). Unchallenged control animals showed increased immune reactivity between 8 and 15 months into the experiment.…”

Section: Discussionmentioning

confidence: 97%

Experimental Infection Model for Johne's Disease in Sheep

et al. 2005

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Johne's disease in ruminants results in chronic enteritis caused by the pathogenic bacterium Mycobacterium avium subsp. paratuberculosis. This study examined two M. avium subsp. paratuberculosis strains (JD3 and W), using different doses and routes of infection, to establish the optimal time postchallenge when predictable levels of infection, gut lesions, and clinical disease occur in a large proportion of sheep. While a small proportion (25%) of sheep challenged with a low-passage-number laboratory culture of M. avium subsp. paratuberculosis (strain W) became infected, no infection was found in animals exposed to a high-passagenumber culture isolate of strain W. In contrast, a primary tissue homogenate of M. avium subsp. paratuberculosis (JD3) resulted in high (90%) infection rates and gut histopathology following oral or intratonsillar challenge. The optimal conditions necessary to produce Johne's disease involve oral inoculation of 3-month-old lambs with four doses of 5 ؋ 10 8 CFU of M. avium subsp. paratuberculosis isolated directly from the gut lymphatic tissues of clinically affected sheep. This resulted in consistent gut histopathology at 9 months and the onset of clinical disease by 11 months postchallenge.

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Section: Discussionmentioning

confidence: 97%

Experimental Infection Model for Johne's Disease in Sheep

et al. 2005

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“…However, the fact that young ruminants have high nonspecific production of IFN-␥ puts constrains on the use of IFN-␥-based diagnostics of bovine paratuberculosis in animals less than 2 years of age and especially less than 1 year of age [32]. A role for the abundantly present ␥␦ T cells in young ruminants is suspected; however, the biology behind these observations remains unclear as is the function of these cells [13,33,34].…”

Section: Discussionmentioning

confidence: 99%

Mycobacterial 70kD heat-shock protein is an effective subunit vaccine against bovine paratuberculosis

Koets

Hoek

Langelaar

et al. 2006

Vaccine

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Paratuberculosis is a chronic granulomatous inflammation of the small intestine of cattle and other ruminants, caused by infection with Mycobacterium avium ssp. paratuberculosis (MAP). The disease can be found in ruminant herds worldwide, causing substantial economic losses at farm level due to premature culling and production losses.In previous studies, it has been shown that immune responses to recombinant MAP Hsp70 proteins were predominantly cell mediated. As protective immunity to the intracellular mycobacterial pathogens is thought to be cell-mediated in origin, we have studied the use of a recombinant MAP Hsp70 as a subunit vaccine in cattle experimentally infected with MAP.The results of the current study demonstrate that recombinant MAP Hsp70 can be successfully used as a subunit vaccine against bovine paratuberculosis, significantly reducing shedding of bacteria in feces during the first 2 years following experimental infection.

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“…This assay is seemed to be suitable for the identification of subclinically infected animals (Stabel, 1996;Jungersen et al, 2002). On the other hand, the test cannot be considered suitable for the diagnostics of paratuberculosis in general as suggested by Billman-Jacobe et al (1992), although it has proved to be effective as a test for bovine tuberculosis . Our results show clearly that the number of animals with positive IFN-γ test is lower than the number of infected animals and it does not include susceptible animals shedding large amount of germs in faeces (group H) that have low level of cell-mediated immunity (Table 2).…”

Section: Discussionmentioning

confidence: 99%

Immunological characteristics of cattle with Mycobacterium avium subsp. paratuberculosis infection

Toman¹,

Faldyna²,

Pavlík³

2003

Vet. Med. - Czech

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Several years lasting clinical and microbiological monitoring of a cattle herd infected with paratuberculosis allowed to identify three groups of animals showing different courses of the infection. Group N (non-shedders) included animals negative by faecal culture throughout the monitoring period. Animals of Group L (low shedders) shed sporadically small quantities of mycobacteria (1 to 9 CFU), but remained clinically healthy throughout the monitoring period. Group H (high shedders) included animals shedding repeatedly large quantities of Mycobacterium avium subsp. paratuberculosis (10 CFU) with a progressive deterioration of the state of health in most of them. Animals with specific antibodies detected by agar gel immunodiffusion and complement fixation test were found in all groups, but the percentage of serologically positive animals was higher in animals of Group L and significantly higher (p < 0.01) in animals of Group H than in animals of Group N. Specific cell-mediated immunity was demonstrated especially in the group of low shedders (Group L), the differences in the percentages of interferon gamma assay positive animals in this group and other groups was highly significant (p < 0.01). Only insignificant differences in the counts and activity of peripheral leukocytes were found among groups of clinically healthy animals differing in intensity of M. paratuberculosis shedding. However, the progressive development of clinical signs of paratuberculosis in Group H was associated with a significant (p < 0.01) decrease of lymphocytes and monocytes, a non-significant decrease of neutrophils, a significant (p < 0.05) increase in the percentage of CD4<sup>+</sup> cells and a insignificant decrease in the percentage of CD8<sup>+</sup> cells; consequently the CD4/CD8 ratio increased.

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A comparison of the interferon gamma assay with the absorbed ELISA for the diagnosis of Johne's disease in cattle

Cited by 75 publications

References 11 publications

Experimental Infection Model for Johne's Disease in Sheep

Experimental Infection Model for Johne's Disease in Sheep

Mycobacterial 70kD heat-shock protein is an effective subunit vaccine against bovine paratuberculosis

Immunological characteristics of cattle with Mycobacterium avium subsp. paratuberculosis infection

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