A comparison of the influence of material on in vitro cartilage tissue engineering with PCL, PGS, and POC 3D scaffold architecture seeded with chondrocytes

Jeong, Claire G.; Hollister, Scott J.

doi:10.1016/j.biomaterials.2010.01.145

Cited by 118 publications

(110 citation statements)

References 34 publications

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“…Interestingly, after 4 weeks of culture, POC provided the best support for cartilage regeneration: It yielded the highest tissue ingrowth (cell penetration), matrix production, relative mRNA expressions for chondrocyte differentiation (Col2/Col1), and DNA and glycosaminoglycan content. The results (113) demonstrate that POC can outperform other biodegradable elastomers used for cartilage tissue engineering and warrants further in vivo studies. Motivated by their previous studies, these researchers published successive reports to evaluate the coupled effects of 3D POC scaffold pore shape and permeability on chondrogenesis using primary chondrocytes in vivo (114, 115).…”

Section: Applications In Regenerative Engineeringmentioning

confidence: 83%

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Citrate-Based Biomaterials and Their Applications in Regenerative Engineering

Tran

Yang

Ameer

2015

Annu. Rev. Mater. Res.

107

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Advances in biomaterials science and engineering are crucial to translating regenerative engineering, an emerging field that aims to recreate complex tissues, into clinical practice. In this regard, citrate-based biomaterials have become an important tool owing to their versatile material and biological characteristics including unique antioxidant, antimicrobial, adhesive, and fluorescent properties. This review discusses fundamental design considerations, strategies to incorporate unique functionality, and examples of how citrate-based biomaterials can be an enabling technology for regenerative engineering.

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Section: Applications In Regenerative Engineeringmentioning

confidence: 83%

“…3D scaffolds of the same microarchitectural design were fabricated using PCL, PGS, or POC (113). These studies show that PGS is the least favorable material for cartilage regeneration, as determined by high dedifferentiation (Col1), hypertrophic mRNA expression (Col10), and high matrix degradation (MMP13, MMP3).…”

Section: Applications In Regenerative Engineeringmentioning

confidence: 99%

Citrate-Based Biomaterials and Their Applications in Regenerative Engineering

Tran

Yang

Ameer

2015

Annu. Rev. Mater. Res.

107

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“…Although permeability was measured, it was not specifically controlled through scaffold design. Lastly, we have previously shown 39 that PCL scaffolds do not support cartilage regeneration or POC scaffolds for the same architecture. In our study, we designed scaffolds such that pore size, surface area, and degrees of interconnectivity were rigorously controlled within narrow ranges.…”

Section: Jeong and Hollistermentioning

confidence: 96%

Mechanical and Biochemical Assessments of Three-Dimensional Poly(1,8-Octanediol-co-Citrate) Scaffold Pore Shape and Permeability Effects onIn VitroChondrogenesis Using Primary Chondrocytes

Jeong

Hollister

2010

Tissue Engineering Part A

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Poly(1,8-octanediol-co-citrate) (POC) is a biocompatible, biodegradable elastomer with potential application for soft tissue applications such as cartilage. For chondrogenesis, permeability is a scaffold design target that may influence cartilage regeneration. Scaffold permeability is determined by many factors such as pore shape, pore size, pore interconnectivity, porosity, and so on. Our focus in this study was to examine the effects of pore shape and permeability of two different POC scaffold designs on matrix production, mRNA gene expression, and differentiation of chondrocytes in vitro and the consequent mechanical property changes of the scaffold/tissue constructs. Since type I collagen gel was used as a cell carrier in the POC scaffolds, we also examined the effects of collagen gel concentration on chondrogenesis. We found that lower collagen I gel concentration provides a favorable microenvironment for chondrocytes promoting better chondrogenic performance of chondrocytes. With regard to scaffold design, low permeability with a spherical pore shape better enhanced the chondrogenic performance of chondrocytes in terms of matrix production, and mRNA gene expressions in vitro compared to the highly permeable scaffold with a cubical pore shape.

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“…PGS has been examined as a membrane material for cell delivery to damaged tissues, including the heart [4] and retina [5], and as a material for nerve guidance conduits for peripheral nerve repair [6]. Porous PGS scaffolds have been used to support the growth of cardiac tissue [7,8], blood vessels [9,10], and cartilage [11][12][13]. Additionally, PGS has also been used as a degradable drug carrier for antibiotics and anticancer drugs [14,15].…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization and 3D Micro-Structuring via 2-Photon Polymerization of Poly(glycerol sebacate)-Methacrylate–An Elastomeric Degradable Polymer

et al. 2018

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Poly(glycerol sebacate) (PGS) has been utilized in numerous biomaterial applications over recent years. This elastomeric and rapidly degradable polymer is cytocompatible and suited to various applications in soft tissue engineering and drug delivery. Although PGS is simple to synthesize as an insoluble prepolymer, it requires the application of high temperatures for extended periods of time to produce an insoluble matrix. This places limitations on the processing capabilities of PGS and its possible applications. Here, we present a photocurable form of PGS with improved processing capabilities: PGS-methacrylate (PGS-M). By methacrylating the secondary hydroxyl groups of the glycerol units in the PGS prepolymer chains, the material was rendered photocurable and, in combination with a photoinitiator, crosslinked rapidly on exposure to UV light at ambient temperatures. The polymer's molecular weight and the degree of methacrylation could be controlled independently and the mechanical properties of the crosslinked material tailored. The polymer also displayed rapid degradation under physiological conditions and cytocompatibility with various primary cell types. As a demonstration of the processing capabilities of PGS-M, µm scale 3D scaffold structures were fabricated using 2-photon polymerization and used for 3D cell culture. The tunable properties of PGS-M coupled with its enhanced processing capabilities make the polymer an attractive potential biomaterial for various future applications.

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A comparison of the influence of material on in vitro cartilage tissue engineering with PCL, PGS, and POC 3D scaffold architecture seeded with chondrocytes

Cited by 118 publications

References 34 publications

Citrate-Based Biomaterials and Their Applications in Regenerative Engineering

Citrate-Based Biomaterials and Their Applications in Regenerative Engineering

Mechanical and Biochemical Assessments of Three-Dimensional Poly(1,8-Octanediol-co-Citrate) Scaffold Pore Shape and Permeability Effects onIn VitroChondrogenesis Using Primary Chondrocytes

Synthesis, Characterization and 3D Micro-Structuring via 2-Photon Polymerization of Poly(glycerol sebacate)-Methacrylate–An Elastomeric Degradable Polymer

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