“…SKF-525A (2-diethylaminoethyl-2,2-diphenylvalerate-HCl) is one of the most widely used non-selective P450 inhibitors, and has been used to determine whether a drug is metabolized by P450 enzymes (Emoto, Murase, Sawada, Jones, & Iwasaki, 2003;Franklin & Hathaway, 2008). SKF-525A has inhibitory effects against CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6 and CYP3A, but not against CYP1A2, CYP2A6 or CYP2E1 (the IC 50 values for these three are > 50 μM) (Emoto et al, 2003;Emoto, Murase, Sawada, & Iwasaki, 2005;Song et al, 2014). Another useful non-specific P450 inhibitor is 1-aminobenzotriazole (ABT), which is thought to inactivate P450 enzymes following complex formation with metabolic intermediates (MI) (Linder et al, 2009).…”