2013
DOI: 10.4172/2161-0940.1000120
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A Comparison of the Histochemical and Image-Derived Proteoglycan Content of Articular Cartilage

Abstract: There are several methods for determining the proteoglycan content of cartilage in biomechanics experiments. Many of these include assay-based methods and the histochemistry or spectrophotometry protocol where quantification is biochemically determined. More recently a method based on extracting data to quantify proteoglycan content has emerged using the image processing algorithms, e.g., in ImageJ, to process histological micrographs, with advantages including time saving and low cost. However, it is unknown … Show more

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Cited by 4 publications
(3 citation statements)
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“…Cartilage destruction was evaluated by using the OARSI scoring system and by analyzing percentage loss of Safranin O staining and chondrocyte cell loss at the articular surface [Glasson et al, ]. The software ImageJ (NIH) was used to delineate areas of cartilage and Safranin O staining loss [Singh S, ]. Chondrocyte cell density (cellularity) was determined by quantifying the total number of cells per unit area based on the Hematoxylin and Fast green staining results.…”
Section: Methodsmentioning
confidence: 99%
“…Cartilage destruction was evaluated by using the OARSI scoring system and by analyzing percentage loss of Safranin O staining and chondrocyte cell loss at the articular surface [Glasson et al, ]. The software ImageJ (NIH) was used to delineate areas of cartilage and Safranin O staining loss [Singh S, ]. Chondrocyte cell density (cellularity) was determined by quantifying the total number of cells per unit area based on the Hematoxylin and Fast green staining results.…”
Section: Methodsmentioning
confidence: 99%
“…40,41 This was followed by absorbance profiling under monochromatic light source using a Nikon Labo-Phot light microscope to obtain micrographic images of each section before and after staining. A validated image analysis approach 43 was employed to determine the approximate PG content using custom-written program in ImageJ (version 1.45d, Wayne Rasband, National Institute of Health, Bethesda, MD). This was calculated as the area under the Safranin-O absorbance-depth profile, which shows the PG concentration and distribution.…”
Section: Methodsmentioning
confidence: 99%
“…This was calculated as the area under the Safranin-O absorbance-depth profile, which shows the PG concentration and distribution. 43…”
Section: Image Analysis and Proteoglycan Content Approximationmentioning
confidence: 99%