A comparison of the effectiveness of the microscopic method and the multiplex PCR method in identifying and discriminating the species of Nosema spp. spores in worker bees (Apis mellifera) from winter hive debris

Michalczyk, Maria; Sokół, Rajmund; Szczerba‐Turek, Anna; Bancerz‐Kisiel, Agata

doi:10.2478/v10181-011-0058-z

Cited by 15 publications

(18 citation statements)

References 10 publications

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“…Duplex PCR confi rmed the presence of different microsporidia species in worker bees sampled from the same colony on different dates. The above results validate our previous observations [21] that worker bees infected with different Nosema species can inhabit the same colony [25]. Meana et al [26] also demonstrated that the origin of the sampled material plays an important role in the identifi cation of N. ceranae spores.…”

Section: Discussionsupporting

confidence: 80%

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Evaluation of the Effectiveness of Selected Treatments of Nosema Spp. Infection by the Hemocytometric Method and Duplex Pcr

Michalczyk¹,

Sokół²,

Koziatek³

2016

Acta Veterinaria

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Recent years have witnessed an increase in the mortality of honey bees in many regions of the world. The observed decrease in the bee population results from a combination of factors, and microsporidian parasites Nosema apis and N. ceranae are among the main contributors. Those parasites cause a microsporidian infection that shortens the lifespan of bees and reduces the productivity of bee colonies. The aim of this study was to evaluate the effectiveness of Nozevit, Api Herb and ApiX (acetylsalicylic acid + Artemisia absinthium L. extract) in the control of infections caused by Nosema spp. in a fi eld experiment. Two groups of worker bees were evaluated -hive bees and forager bees returning to the hive. The effect of the analyzed therapies on the number of spores and the microsporidia species were analyzed by the hemocytometric method and duplex PCR. A statistical analysis revealed that the applied treatments had reduced the number of spores by 31.15% on average. In hive bees, Nosema spp. infection was most effectively reduced by Nozevit (67.85%) and ApiX (63.36%). Coinfections (N. ceranae and N. apis) were affi rmed in all bee samples before treatments. However, after the treatments, single infection of N. apis and N. ceranae were detected. The tested treatments were more effective in the control of N. apis than N. ceranae.

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Section: Discussionsupporting

confidence: 80%

“…spores in HB from winter hive debris ranged from 13.74 x 10 4 in group N to 25.62 x 10 4 in group Cs (Table 1). The average number of Nosema spp.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the Effectiveness of Selected Treatments of Nosema Spp. Infection by the Hemocytometric Method and Duplex Pcr

Michalczyk¹,

Sokół²,

Koziatek³

2016

Acta Veterinaria

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“…Along with extensive studies on the determination of the species of Nosema spores at the molecular level in the world (23,32,33), the number of studies related to the agent for the disease seems to be high in Turkey; however, these studies have been limited to the general presence …”

Section: Discussionmentioning

confidence: 99%

“…Having used multiplex-PCR diagnosis for Nosema spores and different primer pairs targeting the rRNA gene for discrimination of the species, Michalczyk et al (23) reported that Nosema ceranae was the dominant species in European honeybees. Traver and Fell (24) studied 586 managed A. mellifera colonies from Virginia in 2009 using real-time PCR to detect Nosema apis and Nosema cerenea.…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of Nosema spp. and black queen-cell virusin honeybees in Van Province, Turkey

Oğuz¹,

Karapınar²,

Dinçer³

et al. 2017

Turk J Vet Anim Sci

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This study was planned to determine the prevalences of the Nosema spp. and the black queen-cell virus (BQCV) among honeybees (Apis mellifera) raised in the province of Van by PCR and to determine the molecular characteristics of the determined isolates. A total of 260 adult worker bees from 26 colonies at 5 apiary locations belonging to the province of Van in April and May 2015 were collected for this reason. Samples were examined microscopically. In the case of positivity, spore identification was done by multiplex PCR. Reverse transcription/PCR analysis (RT/PCR) was carried out for the BQCV analysis. At the end of the microscopic examination, Nosema spp. spores were detected in 8 out of 26 colonies (32.5%). The result of multiplex-PCR revealed Nosema ceranae positivity in all of the samples, but no Nosema apis was determined. As a result of the RT/PCR tests of the samples BQCV was detected in 23 (88.5%) of the total 26 colonies. This study is the first to investigate Nosema spp. and BQCV with the PCR technique in bees raised in the province of Van.

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“…The size of the obtained products was evaluated by comparison with the GeneRuler TM 100 bp 36 Ladder Plus (Fermentas) molecular size marker. Electrophoresis results were archived using the GelDoc (Bio-Rad) gel documentation system (Michalczyk et al, 2011).…”

Section: Methodsmentioning

confidence: 99%

Detection of Nosema spp. in Worker Bees of Different Ages During the Flow Season

Sokół¹,

Michalczyk²

2012

Journal of Apicultural Science

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S u m m a r yThe aim of this study was to identify which Nosema species infect those Apis mellifera worker bees performing different functions in the colony. Samples were taken from different places inside and outside the hive, in the honey fl ow season. In February 2010, winter hive debris from 30 colonies was analyzed, and based on the microsporidian species identifi ed by multiplex PCR. The following bee colonies (none of which displayed clinical symptoms of the disease) were selected for further analyses to determine the occurrence of microsporidian parasites: 1) colony A/C infected with Nosema apis and N. ceranae (mixed infection), 2) colony A infected with N. apis, 3) colony C -infected with N. ceranae, and 4) colony K -the control, which was free of infection. Between April and August, 20 nurse bees from frames of open brood, and 20 forager bees returning to the hive from pollen-collecting trips were randomly selected from each colony at 30-day intervals. The results of the study indicate that the microsporidian species is determined not only by the type of worker bee (sampling site), but also by the period (month) of the sample collection. Our fi ndings also suggest that regardless of the type of initial infection, bees infected by different microsporidian species and bees free from infection can coexist in colonies.

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A comparison of the effectiveness of the microscopic method and the multiplex PCR method in identifying and discriminating the species of Nosema spp. spores in worker bees (Apis mellifera) from winter hive debris

Cited by 15 publications

References 10 publications

Evaluation of the Effectiveness of Selected Treatments of Nosema Spp. Infection by the Hemocytometric Method and Duplex Pcr

Evaluation of the Effectiveness of Selected Treatments of Nosema Spp. Infection by the Hemocytometric Method and Duplex Pcr

Molecular detection of Nosema spp. and black queen-cell virusin honeybees in Van Province, Turkey

Detection of Nosema spp. in Worker Bees of Different Ages During the Flow Season

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