A comparison of the effectiveness between amniotic membrane homogenate and transplanted amniotic membrane in healing corneal damage in a rabbit model

Guo, Qing; Hao, Jie; Qian, Yueming; Guan, Lan; Shan, Ouyang; Wang, Jianwen

doi:10.1111/j.1755-3768.2010.02097.x

Cited by 35 publications

(50 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Some studies revealed interesting results concerning homogenates. 9,10,12 Based on a mouse model, our study presented similar results when comparing the mechanical effect to the biological effect.…”

Section: Discussionsupporting

confidence: 65%

“…9,12,13 However, transplantation of AMs involves a substantial risk of the following complications: subconjunctival hemorrhage, early degeneration of the membrane, surface infection, and calcification. 8 Recently, Guo et al 10 compared the efficacy of AM homogenates and transplanted AM for the treatment of alkali burns in rabbits and found that amnion homogenates are as effective as transplanted AM in promoting corneal healing.…”

Section: Methodsmentioning

confidence: 99%

“…[5][6][7][8] In recent years, some studies using homogenates of AMs have also been developed for the treatment of ocular surface disease. 5,6,[8][9][10][11][12] AMs may improve corneal healing through their anti-inflammatory and antiangiogenic properties. 9,12,13 However, transplantation of AMs involves a substantial risk of the following complications: subconjunctival hemorrhage, early degeneration of the membrane, surface infection, and calcification.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Retinoic Acid Engineered Amniotic Membrane Used as Graft or Homogenate: Positive Effects on Corneal Alkali Burns

Joubert

Daniel

Bonnin

et al. 2017

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

PURPOSE. Alkali burns are the most common, severe chemical ocular injuries, their functional prognosis depending on corneal wound healing efficiency. The purpose of our study was to compare the benefits of amniotic membrane (AM) grafts and homogenates for wound healing in the presence or absence of previous all-trans retinoic acid (atRA) treatment. METHODS.Fifty male CD1 mice with reproducible corneal chemical burn were divided into five groups, as follows: group 1 was treated with saline solution; groups 2 and 3 received untreated AM grafts or grafts treated with atRA, respectively; and groups 4 and 5 received untreated AM homogenates or homogenates treated with atRA, respectively. After 7 days of treatment, ulcer area and depth were measured, and vascular endothelial growth factor (VEGF) and matrix metalloproteinase 9 (MMP-9) were quantified.RESULTS. AM induction by atRA was confirmed via quantification of retinoic acid receptor b (RARb), a well-established retinoic acid-induced gene. Significant improvements of corneal wound healing in terms of ulcer area and depth were obtained with both strategies. No major differences were found between the efficiency of AM homogenates and grafts. This positive action was increased when AM was pretreated with atRA. Furthermore, AM induced a decrease in VEGF and MMP-9 levels during the wound healing process. The atRA treatment led to an even greater decrease in the expression of both proteins.CONCLUSIONS. Amnion homogenate is as effective as AM grafts in promoting corneal wound healing in a mouse model. A higher positive effect was obtained with atRA treatment.

show abstract

Section: Discussionsupporting

confidence: 65%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Retinoic Acid Engineered Amniotic Membrane Used as Graft or Homogenate: Positive Effects on Corneal Alkali Burns

Joubert

Daniel

Bonnin

et al. 2017

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

show abstract

“…Interestingly, using a rabbit model of corneal damage, Guo et al. have demonstrated that during a 7‐day follow‐up, AM homogenates displayed a damage‐reducing effectiveness similar to that of regular amnion grafts …”

Section: New Directionsmentioning

confidence: 99%

Amniotic membrane: New concepts for an old dressing

Litwiniuk

Grzela

2014

Wound Repair Regeneration

View full text Add to dashboard Cite

The amniotic membrane is the innermost layer of fetal membranes that surrounds and protects the embryo. The amniotic epithelial cells are a rich source for biologically active factors known to promote cell proliferation and differentiation. Therefore, the amniotic membrane is considered to be an attractive wound dressing material. Despite a large number of publications reporting anti-inflammatory, bacteriostatic, reepithelializing, and scar-preventing properties of amniotic membrane, not all the molecular mechanisms underlying the beneficial actions of the amniotic membrane dressing have been fully elucidated. This review summarizes current knowledge on the properties of the amniotic membrane and its various clinical applications. It includes an overview of the main biologically active factors that may be responsible for the observed clinical effects of amnion dressings. This issue is briefly discussed in the context of the role of amnion processing and inter- and intradonor variations between amniotic membrane specimens. Finally, future directions for the use of amnion derivatives in wound care are indicated.

show abstract

“…[5][6][7][8] Depending on the gravity of the corneal injury, different clinical strategies are proposed, ranging from drug treatments that control inflammation and enhance reepithelialization to the use of amniotic patches in more severe cases. [9][10][11][12][13][14] A better molecular understanding of the different steps in corneal wound healing is still needed. In this context, the receptor for advanced glycation end products (RAGE), identified in 1992 by Neeper et al, is a target of interest because of its involvement in the control of gene transcription during inflammation and reepithelialization processes.…”

mentioning

confidence: 99%

Advanced Glycation End Products and Receptor (RAGE) Promote Wound Healing of Human Corneal Epithelial Cells

Gross

Belville

Lavergne

et al. 2020

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

PURPOSE.We used a human corneal epithelial cell (HCE) line to determine the involvement of the advanced glycation end products (AGEs) / receptor for AGEs (RAGE) couple in corneal epithelium wound healing. METHODS.After wounding, HCE cells were exposed to two major RAGE ligands (HMGB1 and AGEs), and wound healing was evaluated using the in vitro scratch assay. Following wound healing, the HCE cells were used to study the influence of the RAGE ligands on HCE proliferation, invasion, and migration. Activation of the nuclear factor (NF)-κB signaling pathway by the AGEs/RAGE couple was tested using a luciferase reporter assay. Functional transcriptional regulation by this pathway was confirmed by quantification of expression of the connexin 43 target gene. For each experiment, specific RAGE involvement was confirmed by small interfering RNA treatments. RESULTS.AGEs treatment at a dose of 100 μg/mL significantly improved the wound healing process in a RAGE-dependent manner by promoting cell migration, whereas HMGB1 had no effect. No significant influence of the AGEs/RAGE couple was observed on cell proliferation and invasion. However, this treatment induced an early activation of the NF-κB pathway and positively regulated the expression of the target gene, connexin 43, at both the mRNA and protein levels. CONCLUSIONS.Our results demonstrate that the RAGE pathway is activated by AGEs treatment and is involved in the promotion of corneal epithelial wound healing. This positive action is observed only during the early stages of wound healing, as illustrated by the quick activation of the NF-κB pathway and induction of connexin 43 expression.

show abstract

A comparison of the effectiveness between amniotic membrane homogenate and transplanted amniotic membrane in healing corneal damage in a rabbit model

Cited by 35 publications

References 17 publications

Retinoic Acid Engineered Amniotic Membrane Used as Graft or Homogenate: Positive Effects on Corneal Alkali Burns

Retinoic Acid Engineered Amniotic Membrane Used as Graft or Homogenate: Positive Effects on Corneal Alkali Burns

Amniotic membrane: New concepts for an old dressing

Advanced Glycation End Products and Receptor (RAGE) Promote Wound Healing of Human Corneal Epithelial Cells

Contact Info

Product

Resources

About