The main interventional therapeutic techniques of subacromial impingement syndrome (SIS) are subacromial (SA) injection and suprascapular nerve block (SSNB). Also, acromioclavicular joint (ACJ) pathologies may be a common etiological factor with SIS and should be considered in treatment management. This study aimed to investigate the efficacy of SA injection, ACJ and SA injection (ACJ+SA), and SSNB in the treatment of SIS. Material and Methods: This prospective, randomized, controlled trial included 90 patients with chronic shoulder pain associated with SIS. The patients were divided into three groups: Group SA (n=30) receiving ultrasound (US)-guided SA injection, Group SA+ACJ (n=30) receiving US-guided SA+ACJ injection, and Group SSNB (n=30) receiving US-guided SSNB. Follow-up parameters were visual analogue scale (VAS), Shoulder Pain and Disability Index (SPADI), and Short Form-12 (SF-12) scores measured before treatment and at the posttreatment 2 nd week, 4 th , 12 th , and 24 th week. Results: In SA+ACJ and SSNB groups, VAS and SPADI scores were found to be statistically significantly lower than before treatment at all measurement times after treatment (p<0.001). The decrease in both measurement scores did not continue at the 24 th week follow-up of Group SA (p>0.05). The SF-12 scores of all three groups were significantly higher than the baseline at all measurement times (p<0.001). Conclusion: The findings of this study suggest that SA, SA+ACJ injection, and SSNB application have positive effects on pain, function, and quality of life in patients with SIS in the short term, while only SA+ACJ injection and SSNB maintain their effectiveness in long-term followups.