A comparison of the dose and time response of CYP1A1 mRNA induction in chemically treated Atlantic tomcod from two populations

Courtenay, Simon C.; Grunwald, Cheryl; Kreamer, Guat-Lian; Fairchild, Wayne L.; Arsenault, Jacqueline T.; Ikonomou, Michael G.; Wirgin, Isaac

doi:10.1016/s0166-445x(99)00006-5

Cited by 56 publications

(82 citation statements)

References 91 publications

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“…Although tilapia remained in these tanks for only 45 min before being sampled, the fact is that there was a quite rapid adaptative response to carbaryl exposure, compared to controls that did not show any change. There are indeed reports of very rapid gene transcription in fish after toxicant exposure, such as the case of CYPIA1 under the dioxin TCDD activation [36,37].…”

Section: Discussionmentioning

confidence: 99%

Biochemical and histological hepatic changes of Nile tilapia Oreochromis niloticus exposed to carbaryl

Matos

́nhas-Fernandes

Peixoto

et al. 2007

Pesticide Biochemistry and Physiology

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The purpose of this study was to evaluate biochemical and morphological responses induced by carbaryl in the liver of Nile tilapia (Oreochromis niloticus) exposed during 21 days to sublethal concentrations (0.25 and 0.5 mg L À1 ), testing also recover for 14 days in clean water, after 14 days exposure. The activities of the following enzymes were measured: superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), glutathione reductase (GR), and reduced (GSH) and oxidized glutathione (GSSG). Globally, our data showed that exposure to carbaryl decreased the SOD, CAT, GR, and GST activities, except for the SOD and GST activities after 14 days exposure to 0.25 mg L À1. In contrast, after 14 days exposure the GR activity of the hepatic tissue from carbaryl-treated fish showed significant elevation in relation to the control. When fish were left to recover, a positive response was seen in the GSH and GSSG contents. The results of the recovery group suggest that the toxicity produced by carbaryl is reversible to some extent within 15 days. The liver histological analysis showed differences between fish concerning the cellular vacuolization degree (VD) of the hepatocytes. In fish exposed to carbaryl it was observed an increasing hepatocellular basophilia. No other histological alterations were observed when fish was exposed to carbaryl, except a few necrotic foci at day 7. The sections stained with PAS reaction showed that the vacuolization was always not due to glycogen deposits, thus suggesting lipid accumulation. The combined increased basophilia and glycogen depletion is a common, although non-specific, liver response to many toxicants. In short, this work shows a relation between histological and biochemical changes in liver and carbaryl exposure. The effects of carbaryl were observed at different concentrations.

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Section: Discussionmentioning

confidence: 99%

Biochemical and histological hepatic changes of Nile tilapia Oreochromis niloticus exposed to carbaryl

Matos

́nhas-Fernandes

Peixoto

et al. 2007

Pesticide Biochemistry and Physiology

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“…We used PCB126 and TCDD as focal toxicants because of their high level of occur-rence in tissues of other Hudson River fish [26,31], their high toxicities in fish, and their relatively wide use in toxicological studies in other taxa. Several of the macrophenotypic responses that we measured (e.g., body size and eye development in recently hatched larvae) are closely linked to a fish's ability to persist and thrive in nature.…”

Section: Introductionmentioning

confidence: 99%

Toxic effects of PCB126 and TCDD on shortnose sturgeon and Atlantic sturgeon

Chambers

Davis

Habeck

et al. 2012

Enviro Toxic and Chemistry

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Exposure to chemical contaminants is often invoked to explain recruitment failures to populations of sturgeon worldwide, but there is little empirical evidence to support the idea that young sturgeon are sensitive at environmentally relevant concentrations. The authors used shortnose sturgeon (Acipenser brevirostum) and Atlantic sturgeon (Acipenser oxyrinchus) as models to investigate the sensitivities of sturgeon to early-life-stage toxicities from embryonic exposures to graded doses of polychlorinated biphenyl 126 (PCB126) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Survival to hatching of shortnose sturgeon decreased with increasing dose, although the duration of the embryonic period was not significantly altered by exposure in either species. Morphometric features of larvae of both species were affected by dose, including shortening of the body, reduction in head size, reduction in quantity of yolk reserves, and reduction in eye size. Eye development in both species was delayed with increasing dose for both chemicals. The persistence of larvae in a food-free environment decreased inversely with dose in both species, with sharp declines occurring at PCB126 and TCDD doses of ≥1 ppb and ≥0.1 ppb, respectively. Dose-responsive early-life-stage toxicities reported here are among the more sensitive found in fish and occurred at burdens similar to those found in situ in a sympatric bottom-dwelling bony fish in the Hudson River Estuary. The present study is among the first demonstrating the sensitivity of any sturgeon to the hallmark early-life-stage toxicities induced by aryl hydrocarbon receptor agonists.

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“…Determination of the induction, however, at the transcriptional level by means of hybridization utilizing unique nucleotide sequences of the genes is expected to render higher specificity 7,8 . Furthermore, employment of polymerase chain reaction (PCR) technology can substantially increase the sensitivity of the detection process 9,10 …”

Section: Introductionmentioning

confidence: 99%

Two forms of cytochrome P450 cDNA from 3-methylcholanthrene-treated European eel Anguilla anguilla

et al. 2003

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The cytochrome P450 (CYP) represents a large group of microsomal monooxygenases that catalyze drugs as well as a host of lethal environmental contaminants such as dioxins, leading to either detoxification and excretion from the animal or generation of carcinogenic intermediates. In the present study two forms of cDNA were cloned (Eu MC1 and Eu MC2) for European eel CYP1A genes by polymerase chain reaction (PCR) techniques. The cDNA of Eu MC1 was 3368 bp long coding 521 amino acid residues, and that of Eu MC2 was 2464 bp long coding 517 amino acid residues. Identities of deduced amino acid sequences between Eu MC1 and Japanese eel CYP1A1 and that between Eu MC2 and the second form of Japanese eel CYP1A were 98% and 97%, respectively, showing decisively that Eu MC1 and Eu MC2 are orthologous to Japanese eel CYP1A1 and the second form of CYP1A, respectively. A striking difference between the two eel species was that the Eu MC1 peptide was two amino acid residues longer than that of the Japanese eel CYP1A1. Existence of two loci of CYP1A in Japanese and European eels may suggest that the two forms of CYP1A exist widely among the eel species, because the divergence between the two eel species has been shown to be close to the basal divergence among eels. The identities in CYP1A may help to estimate genetic distance between European and Japanese eels.

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A comparison of the dose and time response of CYP1A1 mRNA induction in chemically treated Atlantic tomcod from two populations

Cited by 56 publications

References 91 publications

Biochemical and histological hepatic changes of Nile tilapia Oreochromis niloticus exposed to carbaryl

Biochemical and histological hepatic changes of Nile tilapia Oreochromis niloticus exposed to carbaryl

Toxic effects of PCB126 and TCDD on shortnose sturgeon and Atlantic sturgeon

Two forms of cytochrome P450 cDNA from 3-methylcholanthrene-treated European eel Anguilla anguilla

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