A comparison of the cytotoxic potential of standardized aqueous and ethanolic extracts of a polyherbal mixture comprised of Nigella sativa (seeds), Hemidesmus indicus (roots) and Smilax glabra (rhizome)

Samarakoon, Sameera Ranganath; Thabrew, Ira; Galhena, Prasanna; Silva, Dilip de; Tennekoon, Kamani Hemamala

doi:10.4103/0974-8490.75451

Cited by 53 publications

(46 citation statements)

References 14 publications

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“…MTT assay was carried out as previously described methods [12,13]. Cancer cells plated in 96 well plates were treated with different concentrations (6.25, 12.5, 25, 50 and 100 µg/mL) of compounds and incubated for 24, 48 and 72 h. After incubation, MTT reagent (20 μL of 1 mg/mLstock solution) was added to each well and plates were incubated for 4 h at 37 °C.…”

Section: Cytotoxic Assaymentioning

confidence: 99%

Isolation of cytotoxic triterpenes from the mangrove plant, Scyphiphora hydrophyllacea C.F.Gaertn (Rubiaceae)

Samarakoon¹,

Ediriweera²,

Wijayabandara³

et al. 2018

Trop. J. Pharm Res

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and two-dimensional nuclear magnetic resonance (2D-NMR) and electron ionization-mass spectrometry (EI-MS) techniques. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the cytotoxic effects of the compounds on oestrogen receptor positive breast (MCF-7) and non-small cell lung (NCI-H-292) cancer cells. Results: The isolated compounds were identified as oleanolic acid (1), ursolic acid (2) and eichlerianic acid (3). Ursolic acid and eichlerianic acid showed strong cytotoxic effects {IC50-ursolic acid: 8.47 µg/mL (24 h, MCF-7), 7.78 µg/mL (24 h, NCI-H292) and eichlerianic acid: 8.86 µg/mL (24 h, MCF-7), 10.15 µg/mL (24 h, NCI-H292)} in MCF-7 and NCI-H292 cancer cells at 24, 48 and 72 h post-incubation periods. Conclusion: Hexane and chloroform extracts of the leaves of S. hydrophyllacea yielded three compounds namely oleanolic acid, eichlerianic acid and ursolic acid. Ursolic acid and eichlerianic acid have been isolated for the first time from the leaves of S. hydrophyllacea grown in Sri Lanka and demonstrate in-vitro cytotoxic effects in oestrogen receptor positive (MCF-7) and non-small lung cancer (NCI-H-292) cells.Keywords: Scyphiphora hydrophyllacea, eichlerianic acid, ursolic acid, oleanolic acid, This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest

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Section: Cytotoxic Assaymentioning

confidence: 99%

Isolation of cytotoxic triterpenes from the mangrove plant, Scyphiphora hydrophyllacea C.F.Gaertn (Rubiaceae)

Samarakoon¹,

Ediriweera²,

Wijayabandara³

et al. 2018

Trop. J. Pharm Res

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“…The alcoholic extract of seed showed a significantly higher cytotoxic potential than the seed oil of Petroselinum sativum. This kind of difference in the cytotoxicity of different extracts have also been observed by Samarakoon et al (2010), explaining that this cytotoxicity is possibly due to the active components and herbal preparation (Samarakoon et al, 2010). Alcoholic seed extract (PSA) and seed oil (PSO) of P. sativum were also further investigated to determine their mechanism of cell death by observing them under phase contrast inverted light microscope.…”

Section: Discussionmentioning

confidence: 99%

Anticancer Activity of Petroselinum sativum Seed Extracts on MCF-7 Human Breast Cancer Cells

Farshori¹,

Al-Sheddi²,

Al-Oqail³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Pharmacological and preventive properties of Petroselinum sativum seed extracts are well known, but the anticancer activity of alcoholic extracts and oil of Petroselinum sativum seeds on human breast cancer cells have not been explored so far. Therefore, the present study was designed to investigate the cytotoxic activities of these extracts against MCF-7 cells. Cells were exposed to 10 to 1000 μg/ml of alcoholic seed extract (PSA) and seed oil (PSO) of Petroselinum sativum for 24 h. Post-treatment, percent cell viability was studied by 3-(4, 5-dimethylthiazol-2yl)-2, 5-biphenyl tetrazolium bromide (MTT) and neutral red uptake (NRU) assays, and cellular morphology by phase contrast inverted microscopy. The results showed that PSA and PSO significantly reduced cell viability, and altered the cellular morphology of MCF-7 cells in a concentration dependent manner. Concentrations of 50 μg/ml and above of PSA and 100 μg/ml and above of PSO were found to be cytotoxic in MCF-7 cells. Cell viability at 50, 100, 250, 500 and 1000 μg/ml of PSA was recorded as 81%, 57%, 33%, 8% and 5%, respectively, whereas at 100, 250, 500, and 1000 μg/ml of PSO values were 90%, 78%, 62%, and 8%, respectively by MTT assay. MCF-7 cells exposed to 250, 500 and 1000 μg/ml of PSA and PSO lost their typical morphology and appeared smaller in size. The data revealed that the treatment with PSA and PSO of Petroselinum sativum induced cell death in MCF-7 cells.

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“…Furthermore, another study [50] carried out on standardized aqueous and ethanolic extracts of a plant mixture (Nigella sativa, Hemidesmus indicus, and Smilax glabra) determined their cytotoxic effects on human hepatoma (HepG2) cell lines. The study demonstrated that both extracts exhibited strong dose dependent cytotoxicity to HepG2 cells.…”

Section: Discussionmentioning

confidence: 99%

Ethanolic Extract of Nigella sativa Seeds Lacks the Chemopreventive Efficacy in the Post Initiation Phase of DMH-Induced Colon Cancer in a Rat Model

Asfour¹,

Almadi²,

Haffar³

2013

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Nigella sativa, belonging to the Ranunculacea family, is a versatile phytochemical mine bestowed with multi-dimensional medicinal effects. The current study was performed to investigate the chemopreventive efficacy, if any, of the ethanolic extract of indigenous Nigella sativa seeds (ENS) and to evaluate its potentials on tumor progression during the initiation and post initiation phases of Dimethylhydrazine-induced colon carcinogenesis in a rat model. ENS treatment during the initiation phase unveiled chemopreventive effect manifested by significant reductions in tumor incidence, multiplicity and tumor volumes. Histopathological findings and modified Duke's classification of tumors provided evidence that ENS, administered in the initiation phase, is capable of delaying progression, restricting invasion and attenuating aggressiveness of colon tumors. These results imply that ENS may be a promising candidate in the primary prevention of colon cancer. On the contrary, we demonstrated that ENS lacked chemopreventive and tumorigenesis inhibitory effects in the post initiation phase. We speculate that the chemopreventive effect of ENS might be due to the synergistic actions of various constituents present in the extract. However, extensive studies are warranted and more efforts need to be dedicated to fractionate, analyze and to further appraise the anticancer effect of ENS before any definitive conclusions can be drawn.

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A comparison of the cytotoxic potential of standardized aqueous and ethanolic extracts of a polyherbal mixture comprised of Nigella sativa (seeds), Hemidesmus indicus (roots) and Smilax glabra (rhizome)

Cited by 53 publications

References 14 publications

Isolation of cytotoxic triterpenes from the mangrove plant, <i>Scyphiphora hydrophyllacea</i> C.F.Gaertn (Rubiaceae)

Isolation of cytotoxic triterpenes from the mangrove plant, <i>Scyphiphora hydrophyllacea</i> C.F.Gaertn (Rubiaceae)

Anticancer Activity of Petroselinum sativum Seed Extracts on MCF-7 Human Breast Cancer Cells

Ethanolic Extract of <i>Nigella sativa</i> Seeds Lacks the Chemopreventive Efficacy in the Post Initiation Phase of DMH-Induced Colon Cancer in a Rat Model

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A comparison of the cytotoxic potential of standardized aqueous and ethanolic extracts of a polyherbal mixture comprised of Nigella sativa (seeds), Hemidesmus indicus (roots) and Smilax glabra (rhizome)

Cited by 53 publications

References 14 publications

Isolation of cytotoxic triterpenes from the mangrove plant, <i>Scyphiphora hydrophyllacea</i> C.F.Gaertn (Rubiaceae)

Isolation of cytotoxic triterpenes from the mangrove plant, <i>Scyphiphora hydrophyllacea</i> C.F.Gaertn (Rubiaceae)

Anticancer Activity of Petroselinum sativum Seed Extracts on MCF-7 Human Breast Cancer Cells

Ethanolic Extract of &lt;i&gt;Nigella sativa&lt;/i&gt; Seeds Lacks the Chemopreventive Efficacy in the Post Initiation Phase of DMH-Induced Colon Cancer in a Rat Model

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Ethanolic Extract of <i>Nigella sativa</i> Seeds Lacks the Chemopreventive Efficacy in the Post Initiation Phase of DMH-Induced Colon Cancer in a Rat Model