A Comparison of the Chromosome Damaging Effects, in Cultured Human Lymphocytes, of Benzidine (BZD) and 4,4″-Diaminoterphenyl (DAT) in the Presence of Aroclor-induced and Uninduced Rat Liver S9

Asquith, J.C.; Hogan, L. K.; Fullwood, J. N.; Rae, J. D.; Kirkland, David

doi:10.1007/978-1-349-07901-8_42

Cited by 2 publications

(3 citation statements)

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“…The in vitro assay results for benzidine following activation by $9 with and without induction by Aroclor 1254 are given in Table 1. Asquith et al (1985), in their study of CA in human lymphocyte cultures, reported a greater response for activation with uninduced $9 than for induced $9. In the present study with mouse lymphocytes, a concentration-response relationship was found with both treatments but they were not significantly different.…”

Section: Resultsmentioning

confidence: 97%

“…Methods have also been developed to follow chromosomal damage in peripheral blood lymphocytes of rodents following exposure to chemicals in vivo (Kligerman et al, 1982;Rithdech et al, 1987). Cultured human blood lymphocytes after in vitro exposure to chemicals have also been examined for chromosomal damage in the absence of $9 activation (Norppa et al, 1981;Sasiadek et al, 1991) and in the presence of $9 activation (Asquith et al, 1985).…”

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confidence: 99%

“…In the present study, we needed to extend this approach to a series of aromatic amines that require metabolic activation prior to exhibiting genotoxicity in vitro. A useful starting point for this study is the work of Asquith et al (1985) where liver $9 fractions were used with the promutagen benzidine to study CA effects in cultured lymphocytes. Thus, in the present investigation, in addition to benzidine, 4-aminobiphenyl, 4-amino-4'-nitrobiphenyl, 4-aminostilbene, 4,4'diaminostilbene and 4-amino-4'-nitrostilbene were examined for CA in mouse lymphocyte cultures following direct in vitro exposure of the amines to the cultures as well as in lymphocyte cultures prepared from mice subsequent to the i.p.…”

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confidence: 99%

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Chromosomal aberrations in mouse lymphocytes exposed in vitro and in vivo to benzidine and 5 related aromatic amines

Das

Hooberman

et al. 1994

Mutation Research/Genetic Toxicology

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Mouse lymphocytes were exposed in vitro for 2 h or in vivo for 24 h to benzidine and related aromatic amines to test for chromosome aberrations (CA) and mitotic indices. Uninduced mouse S9 was used to activate the amines for the in vitro tests to be consistent with the in vivo tests. Contrary to a previous report, no difference could be established in the genotoxicity of benzidine following activation with uninduced S9 compared to induced S9. There were concentration related increases in CA for benzidine and all the amines in vitro except for 4,4'-diaminostilbene which exhibited the greatest cellular toxicity towards cultured lymphocytes. Benzidine and its derivatives showed significant increases in CA in vivo compared to its negative control. The CA values for 4-aminostilbene were significantly higher than the other amines in both in vivo and in vitro studies. These genotoxicity results for 4-aminostilbene are consistent with our previous report of the pronounced CA effects in murine bone-marrow cells but would not be predicted from Salmonella mutagenicity tests.

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Section: Resultsmentioning

confidence: 97%

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confidence: 99%

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confidence: 99%

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Chromosomal aberrations in mouse lymphocytes exposed in vitro and in vivo to benzidine and 5 related aromatic amines

Das

Hooberman

et al. 1994

Mutation Research/Genetic Toxicology

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In Vitro Genotoxic Effects of Sarcocystis gigantea Cystizoites Acetone Powder Extract on Sheep Lymphocytes

Bayati

2021

Iraqi J. Vet. Med.

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The toxin of the protozoan intracellular parasite of sheep Sarcocystis gigantea is associated with many clinical and pathological signs. The aim of the study was to investigate In Vitro various chromosomal aberrations due to sarcocystosis infection. Macrocysts of Sarcocystis gigantea were isolated from local karadi sheep, homogenized with glass Dounce homogenizer; acetone powder was prepared from it and used in various concentration to investigate the chromosomal aberration in vitro against sheep lymphocytes. The direct effects of parasite cystizoites acetone powder revealed various genotoxicity effects. These effects included chromosomal aberration (Isogap, Breaks and Dicentrics) and chromatids aberration (Gap and Deletion). It had also an effect on the mitotic index of the lymphocyte cells division. These genotoxicities were studied for the first time with in vitro technique using sheep lymphocytes. These results reflected that Sarcocystis gigantean parasite could cause structural and internal aberration in the chromosomes of their hosts.

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A Comparison of the Chromosome Damaging Effects, in Cultured Human Lymphocytes, of Benzidine (BZD) and 4,4″-Diaminoterphenyl (DAT) in the Presence of Aroclor-induced and Uninduced Rat Liver S9

Cited by 2 publications

References 4 publications

Chromosomal aberrations in mouse lymphocytes exposed in vitro and in vivo to benzidine and 5 related aromatic amines

Chromosomal aberrations in mouse lymphocytes exposed in vitro and in vivo to benzidine and 5 related aromatic amines

In Vitro Genotoxic Effects of Sarcocystis gigantea Cystizoites Acetone Powder Extract on Sheep Lymphocytes

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