A comparison of the actions of cromakalim and nifedipine on rabbit isolated mesenteric artery

McHarg, Aileen; Southerton, J. S.; Weston, A.H.

doi:10.1016/0014-2999(90)90633-h

Cited by 15 publications

(9 citation statements)

References 20 publications

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“…Indeed, it has been reported that in airway smooth muscle preparations [26], in the rat urinary bladder [27], in the rat vas deferens [28] and in the rabbit portal vein [11], pinacidil inhibits release of neurotransmitters. In contrast, in the guinea‐pig and rabbit mesenteric arteries, pinacidil and cromakalim had no effect on presynaptic neurotransmitter release [29,30]. However, these observations need further evaluation.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Potassium Channel Opener Pinacidil on the Non‐Pregnant Rat Uterus

Novaković

Milovanović²,

Protić

et al. 2007

Basic Clin Pharma Tox

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The effects of the K + channel opener, pinacidil on the spontaneous rhythmic contractions and contractions provoked by electrical field stimulation (50 Hz) or by oxytocin were investigated in the isolated uterus of the non-pregnant rat in oestrus. Pinacidil produced more potent inhibition of oxytocin-elicited contractions than of spontaneous rhythmic contractions or electrical field stimulation-induced contractions. Glibenclamide, a selective blocker of adenosine triphosphate (ATP)-sensitive K + (K ATP ) channels, antagonized the pinacidil-induced inhibition of contractions elicited by oxytocin in a competitive manner. However, the pinacidil-induced inhibition of electrical field stimulation-elicited contractions and spontaneous rhythmic contractions was antagonized non-competitively by glibenclamide. In the uterine strips precontracted with 80 mM K + , the pinacidil-induced maximal relaxation was not affected. The present data show that pinacidil exhibits potent relaxant properties in the rat non-pregnant uterus in oestrus and therefore should be taken into account as a possible agent for treatment of dysmenorrhoea. Based on glibenclamide affinity, it appears that the inhibitory response to pinacidil involves K ATP channels. We need further investigations to explain why the interaction between glibenclamide and pinacidil in this experimental model depends on the nature of contractions. The ability of pinacidil to completely relax the rat non-pregnant uterus pre-contracted with K + -rich solution suggests that K + channel-independent mechanism(s) also play a part in its relaxant effect.

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Section: Discussionmentioning

confidence: 99%

The Effect of Potassium Channel Opener Pinacidil on the Non‐Pregnant Rat Uterus

Novaković

Milovanović²,

Protić

et al. 2007

Basic Clin Pharma Tox

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“…In norepinephrine preconstricted vessels, fenoldopam-induced and PD128907-induced vasorelaxation was increased in vessel preincubated for 30 minutes with nifedipine 25 (10 Ϫ6 M), indicating that the decreased vasorelaxant effects of fenoldopam and PD128907 in norepinephrine-preconstricted vessels may be related to a norepinephrine-induced increase in intracellular calcium ( Figure 6F and G). The vasodilatory effect of PD128907 was abrogated by apamin and charybdotoxin, indicating involvement of smallconductance and/or large-conductance calcium-activated potassium channels ( Figure 6G).…”

Section: Effect Of Antagonistsmentioning

confidence: 97%

“…20,24 To investigate the mechanisms of ligand-mediated vasorelaxation, studies were performed in vessels preincubated for 30 minutes with nifedipine (Sigma) 25 or apamin (Sigma) (10 Ϫ6 M; a small conductance calcium-activated K ϩ channel blocker) 26 and charybdotoxin (Sigma) (10 Ϫ6 M; a large conductance calcium-activated K ϩ channel blocker). 26 To determine the endothelium dependence of any D 1 or D 3 receptor agonist-induced relaxation, vessels with or without endothelium were studied.…”

Section: Mesenteric Artery Studymentioning

confidence: 99%

Dopamine D ₁ Receptor Augmentation of D ₃ Receptor Action in Rat Aortic or Mesenteric Vascular Smooth Muscles

et al. 2004

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Abstract-Dopamine is an important modulator of blood pressure, in part, by regulating vascular resistance. To test the hypothesis that D 1 and D 3 receptors interact in vascular smooth muscle cells, we studied A10 cells, a rat aortic smooth muscle cell line, and rat mesenteric arteries that express both dopamine receptor subtypes. Both D 1 and D 3 receptors are expressed in vascular smooth muscle cells, and the activation of the D 1 receptor relaxes blood vessels and decreases blood pressure. 5,6 However, the effect of D 3 receptors on resistance vessels is not clear. A low intravenous dose of R(ϩ)-7-hydroxydipropyl-aminotetralin (7-OH-DPAT), a D 2 -like agonist with a 50-fold selectivity for the D 3 over the D 2 receptor, constricts postglomerular arterioles without affecting systemic blood pressure. 4 A higher dose, however, decreases blood pressure. 4 To test the hypothesis that D 1 and D 3 receptors interact in vascular smooth muscle cells, we studied the effect of fenoldopam, a D 1 -like receptor agonist, on D 1 and D 3 receptor expressions in rat thoracic aorta-derived smooth muscle cell line (A10). 7 We also studied the effect of D 1 and D 3 receptor agonists on the wall tension of rat mesenteric arterial rings.

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“…In vascular smooth muscle, these compounds open KATp channels which leads to cellular hyperpolarization with a resultant decrease in the amount of free calcium available for smooth muscle contraction. Thus, the reduced intracellular calcium produces vascular relaxation as has been well demonstrated (16,17,19,21).…”

Section: Potassium Channel Openers and Ischemic Injurymentioning

confidence: 87%

U‐89232 [BMS‐189365], a Novel Antiischemic Agent Derived from Cromakalim

Toombs

Shebuski

1994

Cardiovascular Drug Reviews

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A comparison of the actions of cromakalim and nifedipine on rabbit isolated mesenteric artery

Cited by 15 publications

References 20 publications

The Effect of Potassium Channel Opener Pinacidil on the Non‐Pregnant Rat Uterus

The Effect of Potassium Channel Opener Pinacidil on the Non‐Pregnant Rat Uterus

Dopamine D ₁ Receptor Augmentation of D ₃ Receptor Action in Rat Aortic or Mesenteric Vascular Smooth Muscles

U‐89232 [BMS‐189365], a Novel Antiischemic Agent Derived from Cromakalim

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A comparison of the actions of cromakalim and nifedipine on rabbit isolated mesenteric artery

Cited by 15 publications

References 20 publications

The Effect of Potassium Channel Opener Pinacidil on the Non‐Pregnant Rat Uterus

The Effect of Potassium Channel Opener Pinacidil on the Non‐Pregnant Rat Uterus

Dopamine D 1 Receptor Augmentation of D 3 Receptor Action in Rat Aortic or Mesenteric Vascular Smooth Muscles

U‐89232 [BMS‐189365], a Novel Antiischemic Agent Derived from Cromakalim

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Product

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Dopamine D ₁ Receptor Augmentation of D ₃ Receptor Action in Rat Aortic or Mesenteric Vascular Smooth Muscles