A comparison of six different plating media used in the isolation of salmonella

Warburton, Donald W.; Bowen, Barry M.; Konkle, Anne T. M.; Crawford, Cynda; Durzi, Samir Simon; Foster, Roger; Fox, Cathy; Gour, L.; Krohn, Gail; Lacasse, P.; Lamontagne, Gaetan; McDonagh, Shelagh; Arling, Victoria; Mackenzie, Janet; Todd, Ewen C.D.; Oggel, J.; Plante, Robert; Shaw, Susan E.; Tiwari, N. P.; Trottier, Y.; Wheeler, Brenda

doi:10.1016/0168-1605(94)90179-1

Cited by 18 publications

(13 citation statements)

References 22 publications

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“…The agar media above were chosen after an examination of published work and preliminary experiments which demonstrated the superior selectivity of MLCB and XLDN over the traditionally used brilliant green agar and XLD without novobiocin (Fricker and Girdwood 1984 ;Warburton et al 1994).…”

Section: Conventional Culturementioning

confidence: 99%

A comparison of immunomagnetic separation plus enrichment with conventional salmonella culture in the examination of raw sausages

Coleman

Nye

Chick

et al. 1995

Lett Appl Microbiol

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Immunomagnetic separation with additional enrichment was used in conjunction with improved selective media to improve the isolation of salmonellae from raw sausages. The isolation rate achieved was almost double that of conventional culture with no increase in processing time. The selective media gave an overall specificity of approximately 74%; all false-positive pickoffs being identified as Citrobacter freundii. It is believed that this method represents a significant advance in the isolation of salmonellae from foods, although the ideal media both for enrichment and selection have yet to be found.

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Section: Conventional Culturementioning

confidence: 99%

A comparison of immunomagnetic separation plus enrichment with conventional salmonella culture in the examination of raw sausages

Coleman

Nye

Chick

et al. 1995

Lett Appl Microbiol

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“…R10 broth was then dual streak plated (10 l) onto EF-18 agar (Neogen Corp., Lansing, MI) and Rambach agar (CHROMagar, Paris, France) (20,22). Plates were incubated for 24 h at 37°C.…”

mentioning

confidence: 99%

Isolation of Salmonella enterica and Shiga-Toxigenic Escherichia coli O157 from Feces of Animals in Public Contact Areas of United States Zoological Parks

Keen

Durso

Meehan

2007

Appl Environ Microbiol

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“…Coleman et al (1995) found that all such 'false-positive' Salmonella colonies isolated from foods using Mannitol Lysine Crystal Violet Brilliant Green and XLD agar containing novobiocin were Citrobacter freundii. Warburton et al (1994) found that Citrobacter species produced false-positive colonies on five of six selective agars, including XLD, investigated for the isolation of Salmonella. Biochemical confirmation of such presumptive positive isolates can increase test time by up to 2 d and increase the costs of analysis.…”

Section: Introductionmentioning

confidence: 99%

Use of pyrrolidonyl peptidase to distinguishCitrobacterfromSalmonella

Bennett

MacPhee

Betts

et al. 1999

Letters in Applied Microbiology

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In the routine testing of foods for Salmonella, Citrobacter and other members of the Enterobacteriaceae often produce colonies which are almost indistinguishable from Salmonella on commonly used selective agars. Biochemical confirmation of such colonies can be expensive and time‐consuming. It has been suggested that the enzyme pyrrolidonyl peptidase (PYRase) could be used as a rapid test to distinguish Citrobacter colonies (PYRase‐positive) from Salmonella (PYRase‐negative). Pure cultures of Salmonella, Citrobacter and other Enterobacteriaceae were tested for PYRase activity; all strains of Salmonella tested were PYRase‐negative, and all Citrobacter tested were PYRase‐positive. Inoculated and naturally contaminated food samples were tested for the presence of Salmonella by a standard cultural method. A PYR test was used to test Salmonella‐like colonies isolated on selective agar and potentially, eliminate PYR‐positive isolates from further biochemical testing. The test was able to screen out 6% of colonies selected from samples inoculated with Salmonella, and 43% of colonies selected from uninoculated samples.

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A comparison of six different plating media used in the isolation of salmonella

Cited by 18 publications

References 22 publications

A comparison of immunomagnetic separation plus enrichment with conventional salmonella culture in the examination of raw sausages

A comparison of immunomagnetic separation plus enrichment with conventional salmonella culture in the examination of raw sausages

Isolation of Salmonella enterica and Shiga-Toxigenic Escherichia coli O157 from Feces of Animals in Public Contact Areas of United States Zoological Parks

Use of pyrrolidonyl peptidase to distinguishCitrobacterfromSalmonella

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