A Comparison of Screening Tests for Anti-Sperm Activity in the Serum of Infertile Women

Jones, Warren R.; Ing, R. M. Y.; Kaye, Michael

doi:10.1530/jrf.0.0320357

Cited by 33 publications

(4 citation statements)

References 7 publications

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“…Around the year of 1970, Isojima et al 1,2 reported that positive reactions in the SIT were given by sera of 17.2% of the patients with infertility of unexplained cause and not by those of normal pregnant and unmarried women. Later, this observation was also confirmed by other studies 18–22 . However, it should be noted that the result obtained by the SIT, named as sperm‐immobilization value (SIV), is only to be semi‐quantitative.…”

Section: Sex Differences In Anti‐sperm Antibodies (Asa)supporting

confidence: 69%

“…Later, this observation was also confirmed by other studies. [18][19][20][21][22] However, it should be noted that the result obtained by the SIT, named as sperm-immobilization value (SIV), is only to be semi-quantitative. As the SIV cannot quantify the antibody exactly, the strategy for the treatment of infertile women with sperm-immobilizing antibodies is not able to be decided.…”

Section: Measurement and Evaluation Of Asa In Womenmentioning

confidence: 99%

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Sex difference in anti‐sperm antibodies

Shibahara¹,

Chen²,

Honda³

et al. 2022

Reprod Medicine & Biology

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Anti-sperm antibodies (ASA) are one of the causes of immune infertility, and they are produced in some infertile men and women.In the infertility outpatient clinic, the ASA testing for women, such as sperm-immobilization test (SIT). 1,2 is simple for the clinicians because it requires only the patients' sera. In men, however, the world health organization (WHO) recommend to perform the ASA testing for the detection of sperm-bound antibodies simultaneously during the routine semen examination. 3 Due to the complexity, it has been suggested that the actual examinations of ASA for infertile men are rare. Therefore, the option of testing sperm-immobilizing antibody in the infertile patients' sera is discussed for men and women.However, evaluation of sperm-immobilizing antibody in men's sera did not reveal its clinical significance. 4 In this way, it seems to be generally hard to say that the sex difference in ASA is well recognized. For women, sperm are immunogenic and they do not exist in themselves, and ASA are strictly homologous antibodies. On the contrary, ASA are produced as autoantibodies in men. So far, there have been no reports on the

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Section: Sex Differences In Anti‐sperm Antibodies (Asa)supporting

confidence: 69%

Section: Measurement and Evaluation Of Asa In Womenmentioning

confidence: 99%

Sex difference in anti‐sperm antibodies

Shibahara¹,

Chen²,

Honda³

et al. 2022

Reprod Medicine & Biology

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“…Among them, the sperm immobilization test (SIT), which detects sperm‐immobilizing antibodies, has been shown to be the most reliable assay for detecting antisperm antibodies closely related to female infertility 3,4 . This observation was confirmed by other studies 5–8 …”

Section: Introductionmentioning

confidence: 70%

Incidence of Sperm-immobilizing Antibodies in Infertile Women with Past Chlamydia trachomatis Infection

Hirano

Shibahara

Koriyama

et al. 2011

American Journal of Reproductive Immunology

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“…The positive SIT was detected only in sterile women except for one pregnant woman and the highest incidence was 12.4% in the group of sterility of unknown cause. Jones et al 1/202 (0.5%) 0/91 (0%) [41] reported that the incidences of complement-dependent SI-Abs were 121101 (1 1.9%) and 3/37 (8.0 %) in primary and secondary unexplained infertility, respectively, while those in organic infertility and control women were 2/43 (4.6%) and 0/50 (O%), respectively. Petrunia et al [53] also reported quite similar results in screening for sperm antibodies in the sera of sterile women.…”

Section: A Incidence Of Sperm Immobilizing Antibodies (Si-abs) In Sementioning

confidence: 99%

Techniques for Sperm Immobilization Test

Isojima¹,

Koyama²

1989

Archives of Andrology

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Several semiquantitative, quantitative, and microassay techniques had been developed to detect antibodies bound to human spermatozoa: sperm agglutination test (SAT), sperm immobilization test (SIT), immunofluorescence test, radioantiglobulin test, enzyme-linked immunosorbent assay (ELISA), mixed erythrocyte-spermatozoa antiglobulin reaction (MAR), "Panning" test, and immunobead test (IBT). Clinical application of these techniques include (a) detection of sperm immobilizing antibodies in sera of sterile women, (b) follow-up study of sperm immobilizing antibodies, and (c) detection of sperm immobilizing antibodies in cervical mucus and other secretions. The chemical structure of antigen epitope corresponding to Mab H6-3C4 may recognize the internally located repetitive unbranched N-acetyllactosamine structure, regardless of terminal substitution at Gal (i.e., sialyl-i as well as i structure). The majority of sperm-immobilization (SI) positive women's sera were absorbed with carbohydrate components on ejaculated sperm, but only one serum competed with Mab H6-3C4 on binding to sperm except a serum from whom lymphocytes were donated to make Mab H6-3C4. The SI agglutinating antibodies (Abs) in women's sera were raised to the carbohydrate epitopes of glycoprotein in HSP, but epitopes might have several different conformational structures. Studies are in progress to find whether or not SI-Abs could be generated to peptide epitope of human seminal plasma (HSP) or sperm.

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A Comparison of Screening Tests for Anti-Sperm Activity in the Serum of Infertile Women

Cited by 33 publications

References 7 publications

Sex difference in anti‐sperm antibodies

Sex difference in anti‐sperm antibodies

Incidence of Sperm-immobilizing Antibodies in Infertile Women with Past Chlamydia trachomatis Infection

Techniques for Sperm Immobilization Test

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