A comparison of Recovery Methods of Rhamnolipids Produced by Pseudomonas Aeruginosa

Shah, Mansoor Ul Hassan; Sivapragasam, Magaret; Moniruzzaman, Muhammad; Yusup, Suzana

doi:10.1016/j.proeng.2016.06.538

Cited by 38 publications

(22 citation statements)

References 20 publications

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“…The metabolic liquid was subjected to precipitation by adding ammonium sulfate [(NH 4 ) 2 SO 4 ] salts to achieve 65% of saturation and incubated overnight at 4 °C. Then, it was centrifuged at 15,000× g for 15 min and the cell-free metabolic liquid was collected and again centrifuged at 5000× g for 15 min [73]. The supernatant obtained was discarded and the crude biosurfactant was subjected to dialysis with deionized water for 24 h, with exchanges every 4 h to remove the salt adhered to the sample.…”

Section: Methodsmentioning

confidence: 99%

Sustainable biosurfactant produced by Serratia marcescens UCP 1549 and its suitability for agricultural and marine bioremediation applications

et al. 2019

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BackgroundBiosurfactants are surface-active agents produced by microorganisms that have higher efficiency and stability, lower toxicity and higher biocompatibility and biodegradability than chemical surfactants. Despite its properties and potential application in a wide range of environmental and industrial processes, biosurfactants are still not cost-competitive when compared to their synthetic counterparts. Cost effective technologies and renewable raw substrates as agro-industrial and regional waste from northeast of Brazil as cassava flour wastewater, supplemented with lactose and corn oil are mainly the chemically media for growing microorganism and in turn the production of the biosurfactant of quality. This study aimed to obtained biosurfactant by Serratia marcescens UCP 1549 containing cassava flour wastewater (CWW), by application of a full-factorial design, as sustainable practices in puts the production process in promising formulation medium. The characterization of the biomolecule was carried out, as well as the determination of its stability and toxicity for cabbage seeds. In addition, its ability to stimulate seed germination for agriculture application and oil spill bioremediation were investigated.ResultsSerratia marcescens showed higher reduction of surface tension (25.92 mN/m) in the new medium containing 0.2% lactose, 6% cassava flour wastewater and 5% corn waste oil, after 72 h of fermentation at 28 °C and 150 rpm. The substrate cassava flour wastewater showed a promising source of nutrients for biosurfactant production. The isolate biosurfactant exhibited a CMC of 1.5% (w/v) and showed an anionic and polymeric structure, confirmed by infrared spectra. The biomolecule demonstrated high stability under different temperatures, salinity and pH values and non-toxicity against to cabbage seeds. Thus, exploring biosurfactant their potential role in seeds germinations and the promotion and agricultural applications was investigated. In addition, the effectiveness of biosurfactant for removal burned motor oil adsorbed in sand was verified.ConclusionsThe use of medium containing CWW not only reduces the cost of process of biosurfactant production, but also the environmental pollution due to the inappropriate disposal of this residue. This fact, added to the high stability and non-toxicity of the biosurfactant produced by S. marcescens UCP 1549, confirms its high environmental compatibility, make it a sustainable biocompound that can be replace chemical surfactants in diverse industries. In addition, the effectiveness of biosurfactant for stimulate seed germination and removing burned motor oil from sand, suggests its suitability for agriculture and bioremediation applications.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-1046-0) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Sustainable biosurfactant produced by Serratia marcescens UCP 1549 and its suitability for agricultural and marine bioremediation applications

et al. 2019

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“…Purification of rhamnolipids can be achieved using conventional methods including solvent extraction or chromatography while these methods are time consuming. Foam fractionation is an efficient way for rhamnolipids purification [ 106 , 108 , 109 ] and the purity of rhamnolipids will be affected when oil-type carbon sources are used in fermentation as a carbon source as products have similar structures or form complexes with the substrates. Using sugar-type carbon sources will make the down-stream process relatively easy while it is still challenging to obtain pure mono- and di-rhamnolipid, respectively.…”

Section: Challengesmentioning

confidence: 99%

Microbial production of rhamnolipids using sugars as carbon sources

Tan

2018

Microb Cell Fact

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Rhamnolipids are a class of biosurfactants with effective surface-active properties. The high cost of microbial production of rhamnolipids largely affects their commercial applications. To reduce the production post, research has been carried out in screening more powerful strains, engineering microbes with higher biosurfactant yields and exploring cheaper substrates to reduce the production cost. Extensive refining is required for biosurfactant production using oils and oil-containing wastes, necessitating the use of complex and expensive biosurfactant recovery methods such as extraction with solvents or acid precipitation. As raw materials normally can account for 10–30% of the overall production cost, sugars have been proven to be an alternative carbon source for microbial production of rhamnolipids due to its lower costs and straightforward processing techniques. Studies have thus been focused on using tropical agroindustrial crop residues as renewable substrates. Herein, we reviewed studies that are using sugar-containing substrates as carbon sources for producing rhamnolipids. We speculate that sugars derived from agricultural wastes rich in cellulose and sugar-containing wastes are potential carbon sources in fermentation while challenges still remain in large scales.

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“…The effects of these drawbacks can be reduced through the use of agricultural wastes as a carbon source, genetic modification of microorganisms, and growth optimization via computational modelling [3,10,27,28]. With the research interest devoted to the commercial production of biosurfactants, they will rapidly become a competitive substitute for synthetic surfactants, thus promoting the overall health and sustainability of the environment [28,29,30,31,32,33,34,35].…”

Section: Introductionmentioning

confidence: 99%

Microbial Surfactants: The Next Generation Multifunctional Biomolecules for Applications in the Petroleum Industry and Its Associated Environmental Remediation

et al. 2019

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Surfactants are a broad category of tensio-active biomolecules with multifunctional properties applications in diverse industrial sectors and processes. Surfactants are produced synthetically and biologically. The biologically derived surfactants (biosurfactants) are produced from microorganisms, with Pseudomonas aeruginosa, Bacillus subtilis Candida albicans, and Acinetobacter calcoaceticus as dominant species. Rhamnolipids, sophorolipids, mannosylerithritol lipids, surfactin, and emulsan are well known in terms of their biotechnological applications. Biosurfactants can compete with synthetic surfactants in terms of performance, with established advantages over synthetic ones, including eco-friendliness, biodegradability, low toxicity, and stability over a wide variability of environmental factors. However, at present, synthetic surfactants are a preferred option in different industrial applications because of their availability in commercial quantities, unlike biosurfactants. The usage of synthetic surfactants introduces new species of recalcitrant pollutants into the environment and leads to undesired results when a wrong selection of surfactants is made. Substituting synthetic surfactants with biosurfactants resolves these drawbacks, thus interest has been intensified in biosurfactant applications in a wide range of industries hitherto considered as experimental fields. This review, therefore, intends to offer an overview of diverse applications in which biosurfactants have been found to be useful, with emphases on petroleum biotechnology, environmental remediation, and the agriculture sector. The application of biosurfactants in these settings would lead to industrial growth and environmental sustainability.

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A comparison of Recovery Methods of Rhamnolipids Produced by Pseudomonas Aeruginosa

Cited by 38 publications

References 20 publications

Sustainable biosurfactant produced by Serratia marcescens UCP 1549 and its suitability for agricultural and marine bioremediation applications

Sustainable biosurfactant produced by Serratia marcescens UCP 1549 and its suitability for agricultural and marine bioremediation applications

Microbial production of rhamnolipids using sugars as carbon sources

Microbial Surfactants: The Next Generation Multifunctional Biomolecules for Applications in the Petroleum Industry and Its Associated Environmental Remediation

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