A Comparison of Protein Profiles of Cervical Tissue Homogenate, Exfoliated Cells from Cervix and Serum in Normal and Cervical Malignancy Conditions

Abstract

Cervical cancer, the second most common cancer in women, progresses silently over long periods before producing any clinical manifestation. Research in early detection of this condition using proteomic techniques is of very recent origin. We used high-performance liquid chromatography combined with laser-induced fluorescence method in our lab to record the protein profiles of tissue homogenate, cell lysate and serum samples of normal and different stages of malignant conditions of the cervix. Information on protein markers in the protein profiles was derived using various data processing methods including curve resolution. The variations in relative intensities of different peaks with respect to peak height, width and area under the curve from different sample types were compared to get information regarding the concentration of the various proteins and their significance in the induction and metastasis of cervical cancer. The method can be used in diagnosis, follow-up with respect to the progression, remission and effective therapy, in cervical malignancy.

Grape seed proanthocyanidin extract alleviates arsenic-induced lung damage through NF-κB signaling

“…Lung tissue homogenate was prepared as previously described. 34 ROS and LPO in lung tissue ROS and LPO were measured in lung tissue homogenate as previously described. 29 Inflammatory factors in the lung tissue homogenate IL-1b (L151214485), IL-6 (L151210437), IL-10 (L151221646), CRP (L151110355), and TNF-a (151102081) ELISA kits were bought from Cloud-Clone Corp. (Wuhan, China), and used as previously described.…”

Ultra-sensitive high performance liquid chromatography–laser-induced fluorescence based proteomics for clinical applications

“…We have developed an ultra-sensitive high performance liquid chromatography-laser induced fluorescence (HPLC-LIF) detection technique [5,[15][16][17][18][19][20][21][22][23][24] as a protein-profiling approach for analyzing protein profiles of micro-quantities of clinical samples, such as serum [16,19], saliva [20], cellular samples [17] and tissue homogenates [18]. The method is highly objective, capable of diagnosing whether the subject under investigation has normal, pre-malignant, or malignant condition.…”