A Comparison of Patient Matched Meibum and Tear Lipidomes

Brown, Simon H. J.; Kunnen, Carolina; Duchoslav, Eva; Dolla, Naveen K.; Kelso, Michael J.; Papas, Eric; Jara, Percy Lazon de la; Willcox, Mark; Blanksby, Stephen J.; Mitchell, Todd W.

doi:10.1167/iovs.13-12916

Cited by 129 publications

(180 citation statements)

References 29 publications

(49 reference statements)

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“…Techniques are described in detail in the original study 15 , and summarized here. Tears and meibum secretions were collected from four healthy noncontact-lens wearers aged between 20 and 35 years.…”

Section: Methodsmentioning

confidence: 99%

“…A maximum 10 uL of tears was collected in a glass capillary tube placed at the edge of the lower eyelid, transferred to a microcentrifuge tube, centrifuged at low speed Following sample extraction as described previously 15 , quantification of 236 lipid molecular species was performed by chip-based tandem mass spectrometry. Lipid molecular species were normalized to total lipid and principal component analysis (PCA) was performed using MarkerView v1.2.1.1 (Sciex).…”

Section: Methodsmentioning

confidence: 99%

“…To address this question, we have applied a multivariate statistical analysis to investigate the composition of the tear film lipid layer over multiple days in the same subject. In a prior mass-spectrometric tear film lipidomics study by our team, each of four subjects was sampled three times on consecutive days 15 . That study focused on the differences in lipid profile between tears and meibum, and the profiles of individual subjects were averaged and not explored in detail.…”

Section: Introductionmentioning

confidence: 99%

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Intersubject and Interday Variability in Human Tear and Meibum Lipidomes: A Pilot Study

et al. 2016

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Mitchell, T. W. (2016). Intersubject and interday variability in human tear and meibum lipidomes: a pilot study. The Ocular Surface, 14 (1), 43-48. Intersubject and interday variability in human tear and meibum lipidomes: a pilot study Abstract Purpose Our aim was to quantitate day-to-day changes in the tear and meibum lipid profile of individual subjects in a pilot study of healthy humans. Methods Matched tear and meibum samples were obtained from four subjects on three consecutive days. Quantitative lipid profiles of human basal tears and meibum were compared using multivariate analysis by principal components. Results Substantial differences in the lipid profile between subjects were observed, while lipid profiles were steady across the three consecutive days of sampling. Multivariate principal component analysis demonstrated that lysophosphatidylcholine was the largest variant lipid class between subjects in tears, while wax esters comprised the most variation between subjects in meibum secretions. Conclusion Interday variability is shown to be much smaller than interpatient variability, suggesting that tears and meibum subjects both have unique profiles in humans. Disciplines Medicine and Health Sciences Publication DetailsBrown, S. H. J., Kunnen, C. M. E., Papas, E. B., Lazon de la Jara, P., Willcox, M. D. P., Blanksby, S. 2 ABSTRACT Purpose: Our aim was to quantitate day-to-day changes in the tear and meibum lipid profile of individual subjects in a pilot study of healthy humans.Methods: Matched tear and meibum samples were obtained from four subjects on three consecutive days. Quantitative lipid profiles of human basal tears and meibum were compared using multivariate analysis by principal components.Results: Substantial differences in the lipid profile between subjects were observed, while lipid profiles were steady across the three consecutive days of sampling.Multivariate principal component analysis demonstrated that lysophosphatidylcholine was the largest variant lipid class between subjects in tears, while wax ester contributed the most to variation between subjects in meibum secretions.Conclusion: Inter-day variability is shown to be much smaller that inter-patient variability, suggesting that both tears and meibum subjects have unique profiles in individuals.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Intersubject and Interday Variability in Human Tear and Meibum Lipidomes: A Pilot Study

et al. 2016

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“…There is indirect evidence that the compositions of human tear lipids diverge between people of different ethnicities. 62,68 In a study conducted by a research group in Australia, 68 it was found that human tear lipids contain 12%67% polar lipids, such as phospholipids and sphingolipids, and 4.4%60.6% of hydroxyl fatty acids. These components were not found in meibum collected from the same individuals.…”

Section: Tear Filmmentioning

confidence: 99%

“…The fact that most of this study cohort was Chinese might be the reason why there is 5 molar percent less polar lipids identified in this study when compared with the results reported by the Australian researchers. 68 The biophysical properties of tear lipids, such as dynamic interfacial behavior, elasticity, and viscosity, are crucial factors controlling tear film stability. 54,62,70,71 These properties are likely to be closely linked and strongly dependent on tear lipid composition.…”

Section: Tear Filmmentioning

confidence: 99%

Accounting for Ethnicity-Related Differences in Ocular Surface Integrity as a Step Toward Understanding Contact Lens Discomfort

Chan

Svitova

Lin³

2017

Eye &Amp; Contact Lens: Science &Amp; Clinical Practice

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Contact lens discomfort is a common problem that can lead to unsuccessful or limited contact lens wear. Although many factors may contribute to contact lens discomfort, limited research has explored the influence of ethnicity-related differences in the anatomy and physiology of the ocular surface. Therefore, we performed a search of the literature in PubMed using key words related to “ocular surface” paired with the terms “race” and “ethnicity.” The goal of this review was to determine potential areas of research regarding ethnicity differences, particularly between Asian and non-Asian eyes, in ocular surface integrity to advance our understanding of contact lens discomfort.

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A High‐Throughput Method for the Analysis of Erythrocyte Fatty Acids and the Omega‐3 Index

Alqarni

Mcintyre

Brown

et al. 2018

Lipids

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Omega‐3 long‐chain polyunsaturated fatty acids (n‐3 LCPUFA) have several health benefits. In particular, low n‐3 LCPUFA status is associated with cardiovascular disease (CVD) and led to the development of the omega‐3 index that is the proportion of eicosapentaenoic acid and docosahexaenoic acid in the erythrocyte membranes, as a marker of CVD risk. Most methods used to measure the omega‐3 index are laborious and time consuming. Therefore, the aim of this study was to develop a high‐throughput method for the extraction and measurement of erythrocyte fatty acids and the omega‐3 index. For sample extraction and quantification, two methods were used; a single‐step extraction, degradation, and derivatization method by Lepage and Roy, followed by gas chromatography flame ionization detection (GC‐FID), which is commonly used and a high‐throughput method using an automated methyl tert‐butyl ether extraction followed by electrospray ionization mass spectrometry. Both methods were first applied to the analysis of known concentrations of synthetic phospholipid (PL) mixtures to determine recovery and precision prior to their application in the analysis of human erythrocytes. The range of recoveries over five synthetic PL mixtures were 86.4–108.9% and the coefficient of variation was <10% (within‐run) and ≤15.2% (between‐run). Both methods showed high correlation (R = 0.993) for the omega‐3 index and there was no systematic bias in the detection of omega‐3 index using either method. The new high‐throughput method described here offers considerable advantages in terms of simplicity and throughput compared to the GC‐FID method and provides additional information on molecular PL concentrations.

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A Comparison of Patient Matched Meibum and Tear Lipidomes

Cited by 129 publications

References 29 publications

Intersubject and Interday Variability in Human Tear and Meibum Lipidomes: A Pilot Study

Intersubject and Interday Variability in Human Tear and Meibum Lipidomes: A Pilot Study

Accounting for Ethnicity-Related Differences in Ocular Surface Integrity as a Step Toward Understanding Contact Lens Discomfort

A High‐Throughput Method for the Analysis of Erythrocyte Fatty Acids and the Omega‐3 Index

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