A Comparison of Paramyxoviruses by Immunoprecipitation

Abstract

SUMMARYUsing the technique of immune precipitation of [35S]methionine-labelled infected cell polypeptides followed by SDS-polyacrylamide gel electrophoresis and autoradiography it has been shown that SV5 and a closely related isolate are both antigenically related to human parainfluenza virus type 2. Limited cross-reactions were also demonstrated between parainfluenza virus types 1 and 3 by this method and the apparent molecular weights of the major structural components of human parainfluenza virus types 2 and 3 have been deduced.

Comparison between Parainfluenza Virus Type 2 and Simian Virus 5: Monoclonal Antibodies Reveal Major Antigenic Differences

“…3) the overall pattern was very similar and clearly very distinct from that of SV5 (Randall et al, 1987). Thus, in general agreement with other published data on the polypeptides of PF-2 (Goswami & Russell, 1982;Cowley & Barry, 1983), SDS-PAGE analysis showed marked differences in the apparent Mr values of the L, HN, NP and F proteins of SV5 and PF-2, the P and M proteins having similar electrophoretic mobilities. We had previously shown that there was little variation between different human, dog or monkey isolates of SV5 either in terms of the apparent Mr of the virion proteins or in terms of their ability to react with the pool of SV5 MAbs used in this The autoradiogram shown in (a) represents an SDS-PAGE gel exposed for 2 weeks; that shown in (b) was exposed for 10 weeks.…”

“…The results presented in this paper therefore provide evidence that SV5 and PF-2 are distinct species of viruses and are not as closely related antigenically as has been thought (e.g. Goswami & Russell, 1982). However, sequence analysis is required to determine the exact level of evolutionary relatedness of these two viruses.…”

“…On the other hand, while sequence analysis of the F protein gene of mumps virus (Waxham et al, 1987) demonstrated a low level of amino acid sequence homology to both Sendai virus and PF-3 (25~o and 28~o respectively), a greater degree of homology was seen with the F proteins of SV5 (41 ~) and NDV (33 ~). Using polyvalent antisera Goswami & Russell (1982) showed that SV5 and PF-2 were antigenically related, as were PF-1 and PF-3. Ito et a/.…”

“…Furthermore, in a limited study with four MAbs raised to SV5, Goswami & RusseU (1983) showed that both of the MAbs to the NP protein of SV5 also reacted with PF-2 and that one of the two anti-HN MAbs also reacted with PF-2. Thus, although the degree of antigenic relatedness between SV5 and PF-2 is not known it has often been assumed that they are very closely related, and SV5 and PF-2 have even on occasion been classified in the same serotype (Pringle, 1987;Goswami & Russell, 1982). We have previously isolated 54 MAbs to SV5 and used these to show that only minor antigenic variations exist between different simian, canine and human isolates of SV5 (Randall et al, 1987).…”

Molecular relationships between human parainfluenza virus type 2, and simian viruses 41 and 5: determination of nucleoprotein gene sequences of simian viruses 41 and 5

“…Simian virus 5 (SV5) and SV41 were originally isolated from cynomolgus monkey kidney cell cultures (Hull et al, 1958;Miller et al, 1964), and SV5-1ike viruses have been isolated from a number of human sources, including the bone marrow and cerebrospinal fluid of multiple sclerosis patients (Goswami & Russell, 1982;Goswami et al, 1987). Furthermore, about 20~ of individuals in London were shown to be infected with SV5 by serological studies (Goswami et al, 1984).…”