A comparison of morphometric characteristics of sperm from fertile Bos taurus and Bos indicus bulls in Brazil

Beletti, Marcelo Emı́lio; Costa, Luciano da Fontoura; Viana, Matheus P.

doi:10.1016/j.anireprosci.2004.04.019

Cited by 48 publications

(31 citation statements)

References 12 publications

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“…In our study, spring ensured optimum conditions for sperm production with moderate temperatures, humidity and atmospheric pressure. Perturbation of the spermatogenesis process in bulls may lead to abnormalities in sperm chromatin condensation since the loosely condensed sperm chromatin can suffer DNA damage (Beletti et al, 2005;Hallap et al, 2005). A study done by Bochenek et al (2001) showed that defective chromatin structure could be a variable trait affected by disturbances in the spermatogenetic process or external factors such as semen extender, heat stress, or chemicals The differences in the physical and chemical composition of the micro-environments encountered by the spermatozoa may result in the appearance of different motility patterns.…”

Section: Discussionmentioning

confidence: 97%

Seasonal variation in sperm quality parameters in Swedish red dairy bulls used for artificial insemination

Valeanu

Johannisson

Lundeheim³

et al. 2015

Livestock Science

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Section: Discussionmentioning

confidence: 97%

Seasonal variation in sperm quality parameters in Swedish red dairy bulls used for artificial insemination

Valeanu

Johannisson

Lundeheim³

et al. 2015

Livestock Science

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“…After drying, a drop of 0.025% toluidine blue solution in pH 4.0 McIlvaine buffer (citric acidÁsodium phosphate buffer) was placed on the slide, which was immediately covered with a coverslip (Beletti and Mello 2004). After three minutes, 50 digital images were captured of each slide using light microscopy (Olympus BX40) with a 100) immersion objective, which was used to segment by thresholding 100 images of sperm heads per slide (Beletti et al 2005b). …”

Section: In Vitro Capacitationmentioning

confidence: 99%

Chromatin condensation and morphometry of the bovine sperm head afterin vitrosperm selection and capacitation

Campos

Jacomini

Sampaio

et al. 2013

Journal of Applied Animal Research

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Methods of sperm selection and concentration are used to improve efficiency in obtaining viable embryos from in vitro fertilization procedures. Eighteen semen samples were submitted for selection with Percoll gradient and swim-up methods, followed by in vitro sperm capacitation with heparin, to assess the effect of selection method on chromatin condensation and sperm head morphometry. Smears were made at each stage of the process and stained with toluidine blue (TB). Digital images were analysed using computer algorithms developed with Scilab environment. After selection with either the Percoll gradient method sperm had less condensed chromatin. The swim-up method selected for smaller, more symmetrical heads with less variation in size when compared to semen samples that underwent no selection or were selected with the Percoll gradient method. The sperm also exhibited less condensed chromatin and more uniform head shape after heparin-induced capacitation.

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“…Sperm morphology evaluation is undertaken to determine normality and abnormality of sperm cells. The size and shape of spermatozoa are species-specific and different among animals such as cattle (Boersma 2001, Belleti et al 2005 and sheep (Gravance et al 1998, Sancho et al 1998) and such differences are controlled genetically. Maree et al (2010) suggested that sperm morphology can be used to estimate sperm fertility.…”

Section: Introductionmentioning

confidence: 99%

Morphometry and abnormality evaluation of sex-sorted sperm of spotted buffalo (Tedong bonga)

2017

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Abstract. Kaiin EM, Gunawan M, Maulana T. 2017. Morphometry and abnormality evaluation of sex-sorted sperm of spotted buffalo (Tedong bonga). Nusantara Bioscience 9: 175-180. This research aimed to determine the morphometry and the abnormality of the spotted buffallo (Tedong bonga) sperm after the separation of X and Y-chromosome-bearing sperms. Semen was collected from the spotted buffalo bull by using an artificial vagina.The semen quality was evaluated macro-and microscopically. Semen that met the required standard were subjected to separation by using Bovine Serum Albumin (BSA) 5% and 10% column method. The quality of sex-sorted sperm was evaluated microscopically, including motility, concentration, viability, abnormality and intact plasma membrane. Smear preparation using Eosin-Nigrosin dyes was made for sperm morphometry and abnormality evaluation.The evaluation of sperm morphometry and abnormality was conducted on 200 sperm cells for each observation and repeated in triplicates of observation, by using Axiovision Imager Z microscope at objective and ocular magnifications 40x and 10x, respectively.The morphological parameters observed were including the length and the width of head sperm and the head area of sperm X or Y. The abnormality parameter consisted of primary and secondary abnormalities. The data were analyzed descriptively. The results showed that the head size of the Y sperm of spotted buffalo (27.16 µm 2 ) was smaller than that of the X sperm (29.86 µm 2 ) with the abnormality of the Y sperm (7.85%) was also lower than that of the X sperm (9.7%).

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A comparison of morphometric characteristics of sperm from fertile Bos taurus and Bos indicus bulls in Brazil

Cited by 48 publications

References 12 publications

Seasonal variation in sperm quality parameters in Swedish red dairy bulls used for artificial insemination

Seasonal variation in sperm quality parameters in Swedish red dairy bulls used for artificial insemination

Chromatin condensation and morphometry of the bovine sperm head afterin vitrosperm selection and capacitation

Morphometry and abnormality evaluation of sex-sorted sperm of spotted buffalo (Tedong bonga)

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