A Comparison of Loop-Mediated Isothermal Amplification (LAMP) with Other Surveillance Tools for Echinococcus granulosus Diagnosis in Canine Definitive Hosts

Ni, Xing-Wei; McManus, Donald P.; Lou, Zhongzi; Yang, Jifei; Yan, Hong‐Bin; Li, Li; Li, Hongmin; Liu, Quanyuan; Li, Chunhua; Shi, Wan-Gui; Fan, Yan‐Lei; Liu, Xu; Cai, Jinzhong; Lei, Meng-Tong; Fu, Bao‐Quan; Yang, Yurong; Jia, Wan‐Zhong

doi:10.1371/journal.pone.0100877

Cited by 26 publications

(29 citation statements)

References 42 publications

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“…As is discussed further below, molecular diagnosis, including loop-mediated isothermal amplification (LAMP), can be used as a first-line screen for Echinococcus spp. in the field (134)(135)(136)(137) and to detect Echinococcus sp. egg DNA in environmental samples as an important step for identifying high-risk contaminated areas and for defining the actual routes of human infection (138)(139)(140)(141)(142).…”

Section: Figmentioning

confidence: 99%

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Echinococcosis: Advances in the 21st Century

Wen¹,

Vuitton²,

et al. 2019

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SUMMARY Echinococcosis is a zoonosis caused by cestodes of the genus Echinococcus (family Taeniidae). This serious and near-cosmopolitan disease continues to be a significant public health issue, with western China being the area of highest endemicity for both the cystic (CE) and alveolar (AE) forms of echinococcosis. Considerable advances have been made in the 21st century on the genetics, genomics, and molecular epidemiology of the causative parasites, on diagnostic tools, and on treatment techniques and control strategies, including the development and deployment of vaccines. In terms of surgery, new procedures have superseded traditional techniques, and total cystectomy in CE, ex vivo resection with autotransplantation in AE, and percutaneous and perendoscopic procedures in both diseases have improved treatment efficacy and the quality of life of patients. In this review, we summarize recent progress on the biology, epidemiology, diagnosis, management, control, and prevention of CE and AE. Currently there is no alternative drug to albendazole to treat echinococcosis, and new compounds are required urgently. Recently acquired genomic and proteomic information can provide a platform for improving diagnosis and for finding new drug and vaccine targets, with direct impact in the future on the control of echinococcosis, which continues to be a global challenge.

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Section: Figmentioning

confidence: 99%

“…As indicated above, LAMP-based assays are useful as a first-line screen for Echinococcus spp. in the field (134)(135)(136)(137), and PCR methods allow combined identification of definitive host species and Echinococcus sp. infection status using feces collected in the field (260).…”

Section: Prevention and Controlmentioning

confidence: 99%

Echinococcosis: Advances in the 21st Century

Wen¹,

Vuitton²,

et al. 2019

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“…It is characterized by the use of six different primers specifically designed to recognize eight distinct regions on a target gene, with amplification only occurring if all primers bind and form a product [66]. In the last few years, parasitologists have adapted the LAMP approach for the detection of several parasites, including Echinococcus [67][68][69], Taenia [70][71][72], Loa loa [73,74], Opistorchis [75], Clonorchis [76], filariae [77], and Trichinella [78]. The method appears to be promising for S. stercoralis too [79], although it still needs to be validated in clinical practice [13].…”

Section: Possible New Scenariosmentioning

confidence: 99%

Novel approaches to the diagnosis of Strongyloides stercoralis infection

Buonfrate

Formenti

Perandin

et al. 2015

Clinical Microbiology and Infection

185

168

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Strongyloides stercoralis differs from the other soil-transmitted helminths because it puts infected subjects at risk of a fatal syndrome (in cases of immunosuppression for medical conditions, immunosuppressant therapies, or both). Chronic strongyloidiasis is often a non-severe condition, or is sometimes even asymptomatic, but diagnosis and effective therapy are essential in order to eradicate the infection and the life-long risk involved. Therefore, diagnostic methods need to be highly sensitive. Stool microscopy and the Kato-Katz technique are commonly used in prevalence studies, but they are inadequate for S. stercoralis detection. This is probably the main reason why the global prevalence has long been underestimated. Concentration methods, the Baermann technique and Koga agar plate culture have better, but still unsatisfactory, sensitivity. Serological tests have demonstrated higher sensitivity; although some authors have concerns about their specificity, it is possible to define cut-off values over which infection is almost certain. In particular, the luciferase immunoprecipitation system technique combined with a recombinant antigen (NIE) demonstrated a specificity of almost 100%. ELISA coproantigen detection has also shown promising results, but still needs full evaluation. Molecular diagnostic methods are currently available in a few referral centres as in-house techniques. In this review, on the basis of the performance of the different diagnostic methods, we outline diagnostic strategies that could be proposed for different purposes, such as: prevalence studies in endemic areas; individual diagnosis and screening; and monitoring of cure in clinical care and clinical trials.

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“…The reliance on relatively expensive PCR thermal cycler machines is problematic in resource-poor endemic regions, and thus assessment of DNA amplification in normal water bath conditions has been evaluated with loop-mediated isothermal amplification (LAMP) which gave high specificity when parasite tissue or egg-spiked faecal samples were assessed for E. granulosus s.l. and E. granulosus s.s. (Salant et al, 2012;Ni et al, 2014), and also for E. equinus, E. ortleppi, E. canadensis and E. felidis (Wasserman et al, 2014).…”

Section: Copropcr For Detection Of Canine Echinococcosis Caused By Ementioning

confidence: 99%

Echinococcus granulosus: Epidemiology and state-of-the-art of diagnostics in animals

Craig

Mastin

Kesteren

et al. 2015

Veterinary Parasitology

119

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Diagnosis and detection of Echinococcus granulosus (sensu lato) infection in animals is a prerequisite for epidemiological studies and surveillance of echinococcosis in endemic, re-emergent or emergent transmission zones. Advances in diagnostic approaches for definitive hosts and livestock, however, have not progressed equally over the last 20 years. Development of laboratory based diagnostics for canids using coproantigen ELISA and also coproPCR, have had a huge impact on epidemiological studies and more recently on surveillance during hydatid control programmes. In contrast, diagnosis of cystic echinococcosis (CE) in livestock still relies largely on conventional post-mortem inspection, despite a relatively low diagnostic sensitivity especially in early infections, as current serodiagnostics do not provide a sufficiently specific and sensitive practical pre-mortem alternative. As a result, testing of dog faecal samples by coproantigen ELISA, often combined with mass ultrasound screening programmes for human CE, has been the preferred approach for monitoring and surveillance in resource-poor endemic areas and during control schemes. In this article we review the current options and approaches for diagnosis of E. granulosus infection in definitive and animal intermediate hosts (including applications in non-domesticated species) and make conclusions and recommendations for further improvements in diagnosis for use in epidemiological studies and surveillance schemes.

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A Comparison of Loop-Mediated Isothermal Amplification (LAMP) with Other Surveillance Tools for Echinococcus granulosus Diagnosis in Canine Definitive Hosts

Cited by 26 publications

References 42 publications

Echinococcosis: Advances in the 21st Century

Echinococcosis: Advances in the 21st Century

Novel approaches to the diagnosis of Strongyloides stercoralis infection

Echinococcus granulosus: Epidemiology and state-of-the-art of diagnostics in animals

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