“…Experimental sows underwent intrauterine insemination with a dose of 90 ml containing 1x10 9 spermatozoa. Application of even smaller semen doses was described by Weberski et al (1994), Kuster and Althouse (1999), Martinez et al (2001), Watson and Behan, (2002), Dimitrov et al (2007), Fitzgerald et al (2008), and Pelland et al (2008), while Kruger and Rath (2000) used a very low number of spermatozoa in each dose (1x10 6 / ml). Similar results on the fertility of semen were obtained by Estienne et al (2007) when nine different semen extenders were used (Beltsvill Thawing Solution, Merck III, Androhep-lite, Sperm Aid, MR-A, Modena, X-Cell, VSP, Vital) in a dose of 35x10 6 spermatozoa/ml stored at 18°C for seven days.…”