A Comparison of Immersion, Immersion/Oral Combination and Injection Methods for the Vaccination of Channel Catfish Ictalurus punctatus Against Edwardsiella ictaluri

Abstract

Abstract.— Four different vaccination regimes, including non‐vaccinated controls (C), immersion/oral combination (IO). late immersion (LI), and injection (IJ), were evaluated for antibody production and protection from challenge in channel catfish Ictalurus punctatus that were hatched and raised in recirculating culture systems over a 28‐wk period. For IO, fry were vaccinated by immersion on day 12 post‐hatch and given an oral booster during week 10 post‐hatch. The LI and IJ delivered vaccine was also administered at week 10 post‐hatch. No titers were detected in the IO group prior to the oral booster at week 10. Mean titers rose sharply after the week 10 vaccinations in all treatment groups, maintained a high level for about 8 wk, and slowly declined over the next 4 wk. Injected fish had significantly higher titers during the primary response period than either LI or IO fish. There was no statistical difference in titers between LI and IO fish during the primary response. After an oral booster delivered during week 22, titers in LI and IO fish increased significantly at week 25, while titers in IJ fish declined despite the booster delivery. An immersion booster delivered during week 25 resulted in significant increases in titers in all vaccinated groups. Of multiple challenges, the only one not marred by concurrent columnaris infections revealed a low, but significant level of protection in IJ fish at 12 wk post‐vaccination.

Efeito de banhos terapêuticos com formalina sobre indicadores de estresse em tambaqui

“…No tratamento das doenças em peixes, vários protocolos têm sido utilizados, como os banhos terapêuticos, o uso de rações medicadas e a aplicação de vacinas (Thune et al, 1997;Wise & Johnson, 1998;Reed et al, 1999;Wise et al, 2000;Fouz et al, 2001). Em banhos terapêuticos, os produtos químicos mais utilizados no tratamento de doenças são a formalina, o permanganato de potássio e o cloreto de sódio, sal de cozinha (Kabata, 1985;Thatcher, 1991;Pavanelli et al, 1998, Griffin et al, 2002.…”

Humoral immune responses of channel catfish (Ictalurus punctatus) fry and fingerlings exposed toEdwardsiella ictaluri

“…The results of this vaccine were equivocal, possibly due to, at least in part, the young age of the vaccinates. Numerous investigations of the humoral response of channel catfish to killed bacterins have been performed Plumb et al, 1986;Plumb et al, 1994;Thune et al, 1994;Thune et al, 1997;Vinitnantharat & Plumb, 1992;, with varying results. However, no controlled tank studies focusing on the e#ectiveness of vaccinating channel catfish fry have been reported.…”

Efeito do cloreto de sódio sobre as respostas fisiológicas e controle de helmintos monogenóides em tambaqui (Colossoma macropomum)

“…Os protocolos de tratamentos de doenças em peixes comumente empregados consistem na realização de banhos terapêuticos, uso de rações medicadas e aplicação de vacinas (Thune et al 1997;Wise e Johnson 1998;Wise et al 2000;Fouz et al 2001;Wise e Terhune 2001). O sal comum é um dos banhos mais utilizados na profilaxia de peixes, pois, além de reduzir o estresse (Wurts 1995;Gomes et al 2003;Urbinati e Carneiro 2004), tem eficácia comprovada na prevenção e no tratamento de doenças (Pavanelli et al 2008).…”