Abstract:Macromolecular structure determination via diffraction is commonly carried out at cryogenic temperature. Crystals are typically cooled via plunging into liquid cryogens or placing directly into a cold gas stream. Here we compare these two approaches using two different crystal forms of thermolysin. We find that fast plunge cooling of ~300 μm crystals into liquid nitrogen yields higher mosaicities than gas stream cooling using the vial mounting approach (Farley et al., 2014). In some cases low mosaicities can a… Show more
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