A comparison of freezing and thawing methods for the cryopreservation of human semen

Taylor, Patrick J.; Wilson, Judy R.; Laycock, Robert G.; Weger, Janice

doi:10.1016/s0015-0282(16)45985-6

Cited by 24 publications

(9 citation statements)

References 7 publications

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“…Percentage recoveries of 34-76% have been reported by several previous studies (Behrman & Sawada, 1966;Beck & Silverstein, 1975;Keel & Black, 1980;Keel & Karow, 1980;Taylor et al, 1982), and depend to a large extent upon the cryoprotectant and the method of freezing employed. Cohen et al (1981) and Serafini & Marrs (1986), utilizing a similar MEP system, observed percentage survival rates of about 35-36%.…”

Section: Discussionmentioning

confidence: 60%

“…Reduced fertilizing ability of cryopreserved semen has been related to ultrastructural changes (Pederson & Lebeck, 1971), decreased penetration of cervical mucus (Fjallbrant & Ackerman, 1969;Urry et al, 1983) and decreased post-thaw survival (Keel & Black, 1980), and motility Keel & Karow, 1980;Thachil & Jewett, 1981). Several previous studies have examined the efficacy of various cryopreservation techniques for human semen (Zavos et al, 1980;Thachil & Jewett, 1981;Cohen et al, 1981;Taylor et al, 1982;Serafini & Marrs, 1986;Prins & Weidel, 1986). However, few studies have evaluated the effects of freezing on the motility characteristics of spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

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Effects of cryopreservation on the motility characteristics of human spermatozoa

1987

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Ejaculates (164) were obtained from 17 donors serving on an artificial insemination by donor panel. Semen analysis was performed before and after freezing by an integrated microcomputerized system employing the multiple-exposure photography (MEP) method. Sperm count, motility, velocity, motility index (MI; product of the sperm velocity and percentage of motile spermatozoa) and motile density (MD) were determined for each ejaculate. After the initial evaluation the ejaculates were frozen in liquid nitrogen, thawed 24 h later, and assessed for post-thaw motility, velocity, MI and MD. The mean +/- s.e. sperm count and volume for this group of donors was 148 +/- 4 x 10(6)/ml and 3.1 +/- 0.1 ml, respectively. Mean +/- s.e. values obtained from the prefreeze analysis were: motility = 64 +/- 1%, velocity = 30 +/- 0.4 microns/sec, MI = 19 +/- 0.5 microns/sec and MD = 94 +/- 3 x 10(6)/ml. Post-thaw analysis revealed a significant reduction (P less than 0.01) in all values measured. Motility was reduced to 27 +/- 1%, MI was reduced to 5 +/- 0.3 microns/sec, and MD was reduced to 33 +/- 1 x 10(6)/ml. Velocity was the least affected by cryopreservation, being reduced to 21 +/- 0.5 microns/sec (P less than 0.01). Cryopreservation resulted in a marked shift in the frequency distribution of sperm motility and motility index towards subnormal values while in the majority of ejaculates velocity and motile density were maintained in the normal range. Significant differences were noted amongst donors in the percentage change of the various semen measures as a result of cryopreservation. When within-subject coefficients of variation were calculated, velocity was the least variable parameter.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Effects of cryopreservation on the motility characteristics of human spermatozoa

1987

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“…The decrease of the fertility in the exposed man results from asthenospermia and teratospermia. The accepted levels in the general population of the country are: volume, 2.5 ml; motility, 60-90%; counts, >60 milliodml; necrospermia, 50% live; morphology, 25% abnormally shaped [Garcia, 19741. The literature data in a pool of the general population are: volume, 2.5 ml; motility, 60%; counts, >20 and >40 milliodml; morphology, 60% normally shaped and <15% abnormally shaped [Taylor et al, 1982;Brenner et al, 19871. In semen analysis results of 38 men with substantial exposure to lead, 60.5% showed increased absorption and some decrease of the ALA-D activity; there was also a significant increase in the incidence of asthenospermia and teratospermia. These alterations of the spermatic character were similar in groups A, B, and C (Table 11).…”

Section: Discussionmentioning

confidence: 99%

Study of sperm characteristics in persons occupationally exposed to lead

Lerda

1992

American J Industrial Med

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The sperm characteristics of 38 male workers exposed to lead in a battery factory were studied. Sperm analysis was performed after 4 days of sexual abstinence. Parameters analyzed were: volume, sperm count, motility, and morphology. Exposure levels were estimated by measuring the concentration of lead and delta-aminolevulinic acid in the serum. Based on blood lead levels, the tested individuals were divided in three groups: A (12), B (11), and C (15). Significant levels of asthenospermia and teratospermia were found in exposed workers when compared with unexposed controls. Long-term exposure to lead may lead to changes in sperm characteristics and function.

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“…Such individual differences in the response to freezing protocols have also been reported in other species [reviewed in 29] including humans [30, 31], although it has not been explained. There have been strain specific responses to cryopreservation between mouse strains, but not within individuals of inbred strains [32–34].…”

Section: Discussionmentioning

confidence: 73%

Postmortem recovery and cryopreservation of spermatozoa from the vas deferens of rhesus macaques (Macaca mulatta)

2009

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To determine whether sperm derived form the vas deferens could be retrieved and successfully cryopreserved, testes were collected from 20 rhesus macaques (Macaca mulatta). The males ranged in age from 3 to 19 years with an average of 8.5 years. No sperm was obtained from three animals which were younger than 4 years. The remaining 17 samples contained sperm with an average sperm cell number of 421.8 ±88.7 × 106 and an average motility of 72.8 ± 4.4 %. After 24 h of culture in TALP medium at 37° C in 5% CO2 and 95% air, the overall motility decreased significantly in all samples regardless of treatment. Freezing in TEST-yolk buffer containing 6% (vol/vol) glycerol had a significant effect on sperm reducing the immediate post-thaw motility to 42.4 % in non-treated samples. Treatment with db-cAMP and caffeine further reduced sperm motility after 4 hr in fresh sperm (72.8 vs 50.4%), but increased in sperm that had been frozen (14.0 vs 23.2%). The age of the male did not influence sperm concentration or grade, but proved to be a significant factor in determining motility of frozen-thawed treated sperm with lower motility found in samples from older males. Overall the study demonstrates that motile sperm can be obtained from post-mortem males, although subsequent studies will have to determine whether the quality is sufficient to facilitate its use in assisted reproduction.

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A comparison of freezing and thawing methods for the cryopreservation of human semen

Cited by 24 publications

References 7 publications

Effects of cryopreservation on the motility characteristics of human spermatozoa

Effects of cryopreservation on the motility characteristics of human spermatozoa

Study of sperm characteristics in persons occupationally exposed to lead

Postmortem recovery and cryopreservation of spermatozoa from the vas deferens of rhesus macaques (Macaca mulatta)

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