A Comparison of Four Presumptive Tests for the Detection of Blood on Dark Materials

Butler, J. M.; Chaseling, Janet; Wright, Kirsty

doi:10.1111/1556-4029.14091

Cited by 8 publications

(4 citation statements)

References 26 publications

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“…Type of contamination. Finally, six studies examined blood contamination, 35,[40][41][42]44,45 visually and using standardised reagents, [46][47][48] such as leucomalachite green, Kastle-Meyer and luminol. Four considered bacterial contamination culturing bacteria 34,36,37,39 and one both bacterial and fungal, 38 on a range of media.…”

Section: Resultsmentioning

confidence: 99%

“…Much of this research has been informed by forensic pathology. [46][47][48] A key issue to consider is dilution and the potential for under-or overrepresentation of blood products. 47,48 Finally, a power calculation should be performed to ensure that sufficient observations are made to yield statistically significant differences to be detected and clinically significant conclusions drawn.…”

Section: Discussionmentioning

confidence: 99%

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A systematic review of contamination (aerosol, splatter and droplet generation) associated with oral surgery and its relevance to COVID-19

et al. 2020

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Introduction The current COVID-19 pandemic caused by the SARS-CoV-2 virus has impacted the delivery of dental care globally and has led to re-evaluation of infection control standards. However, lack of clarity around what is known and unknown regarding droplet and aerosol generation in dentistry (including oral surgery and extractions), and their relative risk to patients and the dental team, necessitates a review of evidence relating to specific dental procedures. This review is part of a wider body of research exploring the evidence on bioaerosols in dentistry and involves detailed consideration of the risk of contamination in relation to oral surgery. Methods A comprehensive search of Medline (OVID), Embase (OVID), Cochrane Central Register of Controlled Trials, Scopus, Web of Science, LILACS and ClinicalTrials.Gov was conducted using key terms and MeSH (Medical Subject Headings) words relating to the review questions. Methodological quality including sensitivity was assessed using a schema developed to measure quality aspects of studies using a traffic light system to allow inter- and intra-study overview and comparison. A narrative synthesis was conducted for assessment of the included studies and for the synthesis of results. Results Eleven studies on oral surgery (including extractions) were included in the review. They explored microbiological (bacterial and fungal) and blood (visible and/or imperceptible) contamination at the person level (patients, operators and assistants) and/or at a wider environmental level, using settle plates, chemiluminescence reagents or air samplers; all within 1 m of the surgical site. Studies were of generally low to medium quality and highlighted an overall risk of contaminated aerosol, droplet and splatter generation during oral surgery procedures, most notably during removal of impacted teeth using rotatory handpieces. Risk of contamination and spread was increased by factors, including proximity to the operatory site, longer duration of treatment, higher procedural complexity, non-use of an extraoral evacuator and areas involving more frequent contact during treatment. Conclusion A risk of contamination (microbiological, visible and imperceptible blood) to patients, dental team members and the clinical environment is present during oral surgery procedures, including routine extractions. However, the extent of contamination has not been explored fully in relation to time and distance. Variability across studies with regards to the analysis methods used and outcome measures makes it difficult to draw robust conclusions. Further studies with improved methodologies, including higher test sensitivity and consideration of viruses, are required to validate these findings.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A systematic review of contamination (aerosol, splatter and droplet generation) associated with oral surgery and its relevance to COVID-19

et al. 2020

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“…Sensivitas LMG juga dipengaruhi oleh warna substrat kain yang digunakan. LMG menjadi enhancer yang optimal apabila diteteskan pada kain berwarna terang dibandingkan kain yang berwarna gelap [28]. Sensitifitas dan spesifitas bahan laboratorium yang digunakan menjadi penting sebagai indikasi performa uji presumtif yang optimal.…”

Section: Pembahasanunclassified

Pemeriksaan golongan darah sistem absorpsi-elusi pada sampel darah kering

Mayangsari¹,

Nurfaizah

Aziz³

et al. 2022

Filogeni: J. Mahasiswa Bio.

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Penggolongan darah sistem ABO lazim digunakan untuk melacak berbagai kasus bencana, kecelakaan, kejahatan atau paternitas dengan barang bukti darah. Penelitian ini bertujuan untuk memeriksa sampel darah kering bukti kasus dan penggolongan darah metode absorpsi-elusi dengan optimalisasi waktu suhu inkubasi. Darah kering ditetesi dengan antisera A, B dan larutan lektin O, diinkubasi pada suhu 0°C selama 15 menit kemudian dicuci dengan NaCl Fis-0,9 %, dan sentrifugasi 200 rpm selama 10 menit. Larutan eritrosit ABO 2% diteteskan pada tabung reaksi, kemudian pengamatan aglutinasi divisualisasikan dengan mikroskop compound. Hasil penelitian menunjukkan bahwa proses aglutinasi positif ditemukan pada golongan darah O, yaitu berupa gumpalan yang banyak, halus dan cairan agak keruh. Pengamatan mikroskop menunjukkan terjadinya penumpukan sel darah merah pada sampel A. Hal ini menunjukkan bahwa adanya keterkaitan antara sampel A dengan sampel B.

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“…Furthermore, Butler et al [50] compared four methods that have been previously used as presumptive tests for the detection of traces of blood on dark materials, finding that luminol was, overall, the most effective method.…”

mentioning

confidence: 99%

DNA Transfer in Forensic Science: Recent Progress towards Meeting Challenges

Oorschot

Meakin

Kokshoorn

et al. 2021

Genes

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Understanding the factors that may impact the transfer, persistence, prevalence and recovery of DNA (DNA-TPPR), and the availability of data to assign probabilities to DNA quantities and profile types being obtained given particular scenarios and circumstances, is paramount when performing, and giving guidance on, evaluations of DNA findings given activity level propositions (activity level evaluations). In late 2018 and early 2019, three major reviews were published on aspects of DNA-TPPR, with each advocating the need for further research and other actions to support the conduct of DNA-related activity level evaluations. Here, we look at how challenges are being met, primarily by providing a synopsis of DNA-TPPR-related articles published since the conduct of these reviews and briefly exploring some of the actions taken by industry stakeholders towards addressing identified gaps. Much has been carried out in recent years, and efforts continue, to meet the challenges to continually improve the capacity of forensic experts to provide the guidance sought by the judiciary with respect to the transfer of DNA.

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A Comparison of Four Presumptive Tests for the Detection of Blood on Dark Materials

Cited by 8 publications

References 26 publications

A systematic review of contamination (aerosol, splatter and droplet generation) associated with oral surgery and its relevance to COVID-19

A systematic review of contamination (aerosol, splatter and droplet generation) associated with oral surgery and its relevance to COVID-19

Pemeriksaan golongan darah sistem absorpsi-elusi pada sampel darah kering

DNA Transfer in Forensic Science: Recent Progress towards Meeting Challenges

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