A comparison of efficacy and toxicity between electroporation and adenoviral gene transfer

Lefesvre, Pierre; Attema, Joline; Bekkum, Dirk van

doi:10.1186/1471-2199-3-12

Cited by 42 publications

(22 citation statements)

References 25 publications

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“…4,7-9 Nanoinjection requires cell activity, 10,11 centrifugation, 12 AFM operation 13 or electroporation 14 in order to guarantee intracellular interaction. Cell activity is highly dependent on cell type and its environment, and requires prolonged interfacing; 1 electroporation is accompanied by high cytotoxicity and immune response, 15,16 while centrifugation and AFM operation are only applicable to cultures.…”

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confidence: 99%

Biodegradable Nanoneedles for Localized Delivery of Nanoparticles in Vivo: Exploring the Biointerface

Chiappini¹,

et al. 2015

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Nanoneedles display potential in mediating the delivery of drugs and biologicals, as well as intracellular sensing and single cell stimulation through direct access to the cell cytoplasm. Nanoneedles enable cytosolic delivery, negotiating the cell membrane and the endolysosomal system, thus overcoming these major obstacles to the efficacy of nanotherapeutics. The low toxicity and minimal invasiveness of nanoneedles has a potential for the sustained non-immunogenic delivery of payloads in vivo, provided that the development of biocompatible nanoneedles with a simple deployment strategy is achieved. Here we present a mesoporous silicon nanoneedle array that achieves a tight interface with the cell, rapidly negotiating local biological barriers to grant temporary access to the cytosol with minimal impact on cell viability. The tightness of this interfacing enables both delivery of cell-impermeant quantum dots in vivo and live intracellular sensing of pH. Dissecting the biointerface over time elucidated the dynamics of cell association and nanoneedle biodegradation, showing rapid interfacing leading to cytosolic payload delivery within less than 30 minutes in vitro. The rapid and simple application of nanoneedles in vivo to the surface of tissues with different architectures invariably resulted in the localized delivery of quantum dots to the superficial cells and their prolonged retention. This investigation provides an understanding of the dynamics of nanoneedles’ biointerface and delivery outlining a strategy for highly local intracellular delivery of nanoparticles and cell-impermeant payloads within live tissues.

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confidence: 99%

Biodegradable Nanoneedles for Localized Delivery of Nanoparticles in Vivo: Exploring the Biointerface

Chiappini¹,

et al. 2015

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“…These findings are consistent with several studies, demonstrating no detrimental effect of rAd injections into skeletal muscle (Kimura et al ., 2001; Lefesvre et al ., 2002). …”

Section: Discussionmentioning

confidence: 99%

Myosin light chain 3f attenuates age‐induced decline in contractile velocity in MHC type II single muscle fibers

Kim¹,

Torgerud²,

Mosser³

et al. 2011

Aging Cell

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Summary Aging is characterized by a progressive loss of muscle mass and impaired contractility (e.g., decline in force, velocity, and power). Although the slowing of contraction speed in aging muscle is well described, the underlying molecular mechanisms responsible for the decrement in speed are unknown. Myosin heavy chain (MHC) isoforms are the primary molecules determining contractile velocity; however, the contraction speed of single fibers within a given MHC isoform type is variable. Recent evidence proposes that the decline in shortening velocity (Vo) with aging is associated with a decrease in the relative content of essential myosin light chain 3f (MLC3f) isoform. In the current study, we first evaluated the relative content of MLC3f isoform and Vo in adult and old rats. We then used recombinant adenovirus (rAd) gene transfer technology to increase MLC3f protein content in the MHC type II semimembranosus muscle (SM). We hypothesized that (i) aging would decrease the relative MLC3f content and Vo in type II fibers, and (ii) increasing the MLC3f content would restore the age-induced decline in Vo. We found that there was an age-related decrement in relative MLC3f content and Vo in MHC type II fibers. Increasing MLC3f content, as indicated by greater % MLC3f and MLC3f/MLC2f ratio, provided significant protection against age-induced decline in Vo without influencing fiber diameter, force generation, MHC isoform distribution, or causing cellular damage. To the best of our knowledge, these are the first data to demonstrate positive effects of MLC3f against slowing of contractile function in aged skeletal muscle.

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“…Another promising physical delivery method is electroporation, where exposure of cells in culture to electric pulses leads to the formation of transient aqueous pores in the cell membrane, through which enhanced uptake of pDNA is facilitated [27]. Electroporation was subsequently shown to increase the efficiency of pDNA uptake in a variety of gene transfer models [28,29] and has been widely used in skeletal muscle [30][31][32][33][34], where gene expression has been increased by 10-to 100-fold in comparison to injection of naked pDNA alone. Similar improvements in gene expression have also been observed in other tissues such as the liver [35], solid tumours [36,37] and cartilage [38].…”

Section: Introductionmentioning

confidence: 99%

Electroporation enhances reporter gene expression following delivery of naked plasmid DNA to the lung

Pringle

McLachlan

Collie

et al. 2007

The Journal of Gene Medicine

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A comparison of efficacy and toxicity between electroporation and adenoviral gene transfer

Cited by 42 publications

References 25 publications

Biodegradable Nanoneedles for Localized Delivery of Nanoparticles in Vivo: Exploring the Biointerface

Biodegradable Nanoneedles for Localized Delivery of Nanoparticles in Vivo: Exploring the Biointerface

Myosin light chain 3f attenuates age‐induced decline in contractile velocity in MHC type II single muscle fibers

Electroporation enhances reporter gene expression following delivery of naked plasmid DNA to the lung

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