A Comparison of Effects of Broad-Spectrum Antibiotics and Biosurfactants on Established Bacterial Biofilms

Quinn, Gerry A.; Maloy, Aaron P.; Banat, Malik M.; Banat, İbrahim M.

doi:10.1007/s00284-013-0412-8

Cited by 52 publications

(43 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A recent report found no anti-biofilm activity of chloramphenicol against Staphylococcus aureus , E. coli , and Micrococcus luteus . Importantly, the authors only used a concentration of only 0.005 mg/ml, a concentration well below the planktonic MIC for our bacterium and only slightly higher than the MICs for their three isolates (maximum 0.0031 mg/ml) [28]. In contrast, our study shows that high doses (0.08 and 0.80 mg/ml) of chloramphenicol reduce the mass of preformed S. marcescens biofilms, and the 0.80 mg/ml concentration decreases the viability of biofilms compared with untreated controls.…”

Section: Discussionmentioning

confidence: 99%

Killing of Serratia marcescens biofilms with chloramphenicol

Ray

Shenoy

Orihuela

2017

Ann Clin Microbiol Antimicrob

View full text Add to dashboard Cite

Serratia marcescens is a Gram-negative bacterium with proven resistance to multiple antibiotics and causative of catheter-associated infections. Bacterial colonization of catheters mainly involves the formation of biofilm. The objectives of this study were to explore the susceptibility of S. marcescens biofilms to high doses of common antibiotics and non-antimicrobial agents. Biofilms formed by a clinical isolate of S. marcescens were treated with ceftriaxone, kanamycin, gentamicin, and chloramphenicol at doses corresponding to 10, 100 and 1000 times their planktonic minimum inhibitory concentration. In addition, biofilms were also treated with chemical compounds such as polysorbate-80 and ursolic acid. S. marcescens demonstrated susceptibility to ceftriaxone, kanamycin, gentamicin, and chloramphenicol in its planktonic form, however, only chloramphenicol reduced both biofilm biomass and biofilm viability. Polysorbate-80 and ursolic acid had minimal to no effect on either planktonic and biofilm grown S. marcescens. Our results suggest that supratherapeutic doses of chloramphenicol can be used effectively against established S. marcescens biofilms.Electronic supplementary materialThe online version of this article (doi:10.1186/s12941-017-0192-2) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Killing of Serratia marcescens biofilms with chloramphenicol

Ray

Shenoy

Orihuela

2017

Ann Clin Microbiol Antimicrob

View full text Add to dashboard Cite

show abstract

“…Isolates stored at Ϫ80°C were grown on LB agar and incubated at 37°C. A single colony was selected and placed into 3 ml of LB (10 [31]) were also included.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Efficacy of Nonantibiotic Treatments on Biofilm Disruption of Gram-Negative Pathogens and an In Vivo Model of Infectious Endometritis Utilizing Isolates from the Equine Uterus

et al. 2016

View full text Add to dashboard Cite

In this study, we evaluated the ability of the equine clinical treatments N-acetylcysteine, EDTA, and hydrogen peroxide to disrupt in vitro biofilms and kill equine reproductive pathogens (Escherichia coli, Pseudomonas aeruginosa, or Klebsiella pneumoniae) isolated from clinical cases. N-acetylcysteine (3.3%) decreased biofilm biomass and killed bacteria within the biofilms of E. coli isolates. The CFU of recoverable P. aeruginosa and K. pneumoniae isolates were decreased, but the biofilm biomass was unchanged. Exposure to hydrogen peroxide (1%) decreased the biofilm biomass and reduced the CFU of E. coli isolates, K. pneumoniae isolates were observed to have a reduction in CFU, and minimal effects were observed for P. aeruginosa isolates. Chelating agents (EDTA formulations) reduced E. coli CFU but were ineffective at disrupting preformed biofilms or decreasing the CFU of P. aeruginosa and K. pneumoniae within a biofilm. No single nonantibiotic treatment commonly used in equine veterinary practice was able to reduce the CFU and biofilm biomass of all three Gram-negative species of bacteria evaluated. An in vivo equine model of infectious endometritis was also developed to monitor biofilm formation, utilizing bioluminescence imaging with equine P. aeruginosa isolates from this study. Following infection, the endometrial surface contained focal areas of bacterial growth encased in a strongly adherent "biofilm-like" matrix, suggesting that biofilms are present during clinical cases of infectious equine endometritis. Our results indicate that Gram-negative bacteria isolated from the equine uterus are capable of producing a biofilm in vitro, and P. aeruginosa is capable of producing biofilm-like material in vivo. Bacterial endometritis is an important cause of subfertility in mares (1). Endometrial infections are reported in 25 to 60% of mares who fail to become pregnant following breeding (1), contributing to a major economic loss for the equine industry (1, 2). The most common species of bacteria identified during the clinical diagnosis of bacterial endometritis include Streptococcus equi subsp. zooepidemicus, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa (3, 4). The isolation of one of these species of bacteria is considered to be clinically relevant due to observed reductions in pregnancy rates after identification (2). The detection of these bacteria in the uterus would result in treatment with uterine lavage and broad-spectrum antibiotics to reduce bacterial load and eradicate the remaining bacteria (5, 6).Bacterial endometritis that is refractory to traditional antimicrobial treatment is a significant challenge in the equine breeding industry (4). One possible explanation often cited for the failure of antibiotic treatment is the growth of bacterial pathogens in a biofilm (1, 5). Uterine isolates of E. coli, P. aeruginosa, and K. pneumoniae have been proposed to most likely be associated with a biofilm, due to the observation of repeated antibiotic treatment failures in equine reproducti...

show abstract

“…It has also been reported that they are able to alter a substrate's surface energy (Busscher and van der Mei 1997) and increase surface area (Rodrigues et al 2006), which would make biosurfactants important potential candidates for oral health-related applications. A direct application of the latter properties is adjuvant delivery of antibiotics using biosurfactants, resulting in a reduced therapeutic dosage of antibiotics (Quinn et al 2013). In this context, sophorolipids demonstrated potency when delivered in a pharmaceutical formulation where they acted synergistically with antibiotics such as Tetracycline and Cefaclor showing an increase in the permeability of the antibiotics across the outer membrane of the bacteria (Joshi-Navare and Prabhune 2013).…”

Section: Potential Biosurfactant Applications For Oral Health and Hygmentioning

confidence: 99%

Biosurfactants: promising bioactive molecules for oral-related health applications

Elshikh¹,

Marchant²,

Banat³

2016

FEMS Microbiology Letters

Self Cite

View full text Add to dashboard Cite

One sentence summary: Biosurfactant properties including antimicrobial activity, emulsion formation and the ability to increase bioavailability of hydrophobic compounds make them potential candidates for use in cosmetic, therapeutic, oral hygiene and medical devices. Editor: Geertje van Keulen ABSTRACTBiosurfactants are naturally produced molecules that demonstrate potentially useful properties such as the ability to reduce surface tensions between different phases. Besides having similar properties to their artificial chemical counterparts, they are regarded as environmental friendly, biodegradable and less toxic, which make them desirable candidates for downstream applications. The structure-activity-related properties of the biosurfactants which are directly correlated with potency of the biosurfactants as antimicrobial agents, the ability of the biosurfactants to alter surface energies and their ability to increase bioavailability are particularly what attract researchers to exploit their potential use in the oral-related health applications. Current research into biosurfactant indicates significant future potential for use in cosmetic and therapeutic oral hygiene product formulations and related medical device treatments.

show abstract

A Comparison of Effects of Broad-Spectrum Antibiotics and Biosurfactants on Established Bacterial Biofilms

Cited by 52 publications

References 46 publications

Killing of Serratia marcescens biofilms with chloramphenicol

Killing of Serratia marcescens biofilms with chloramphenicol

In Vitro Efficacy of Nonantibiotic Treatments on Biofilm Disruption of Gram-Negative Pathogens and an In Vivo Model of Infectious Endometritis Utilizing Isolates from the Equine Uterus

Biosurfactants: promising bioactive molecules for oral-related health applications

Contact Info

Product

Resources

About