A comparison of Drosophila melanogaster detoxification gene induction responses for six insecticides, caffeine and phenobarbital

Willoughby, Lee; Chung, Henry; Lumb, Chris; Robin, Charles; Batterham, Philip; Daborn, Phillip J.

doi:10.1016/j.ibmb.2006.09.004

Cited by 152 publications

(171 citation statements)

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“…S1). Nine P450s are detected in the fat body, including the previously characterized Cyp6g1, Cyp12d1, and Cyp314a1 (22,40,49). Cyp4p2 and Cyp4s3 are the only P450s detected exclusively in the fat body.…”

mentioning

confidence: 82%

“…It is possible that dGATAe has a role in the regulation of these P450s in the larval midgut. Secondly, exposure of y; cn bw sp larvae to phenobarbital had shown that 21 P450s are induced by this regime (40). This list of 21 P450s includes 16 P450s, which are either expressed in the midgut or Malpighian tubules, suggesting that the induction response mainly occurs in these tissues (Table S4).…”

Section: Temporal Expression Differences Of P450 Between Embryos Andmentioning

confidence: 99%

“…Other microarray experiments performed by challenging D. melanogaster to a wide variety of conditions, such as exposure phenobarbital (40)(41)(42)76), ethanol (77,78), starvation (79), and looking at transcriptome changes in response to aging (80) identified a number of P450s that alter expression levels in response to variations in these parameters. We highlight 2 examples in which tissue-expression data can be used to extend microarray results.…”

Section: Temporal Expression Differences Of P450 Between Embryos Andmentioning

confidence: 99%

“…Approaches, such as sex-specific expression of P450s, induction capacity of P450s by various exogenous compounds, and transcriptional profiling have been useful in characterizing P450 functions (39)(40)(41)(42). In an effort to distinguish P450s with essential endogenous functions from those involved in detoxification, we characterized expression patterns of D. melanogaster P450s by in situ hybridization and performed a P450 RNA interference (RNAi) knockdown screen.…”

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confidence: 99%

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Characterization of Drosophila melanogaster cytochrome P450 genes

Chung

Sztal

Pasricha

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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Cytochrome P450s form a large and diverse family of hemecontaining proteins capable of carrying out many different enzymatic reactions. In both mammals and plants, some P450s are known to carry out reactions essential for processes such as hormone synthesis, while other P450s are involved in the detoxification of environmental compounds. In general, functions of insect P450s are less well understood. We characterized Drosophila melanogaster P450 expression patterns in embryos and 2 stages of third instar larvae. We identified numerous P450s expressed in the fat body, Malpighian (renal) tubules, and in distinct regions of the midgut, consistent with hypothesized roles in detoxification processes, and other P450s expressed in organs such as the gonads, corpora allata, oenocytes, hindgut, and brain. Combining expression pattern data with an RNA interference lethality screen of individual P450s, we identify candidate P450s essential for developmental processes and distinguish them from P450s with potential functions in detoxification.detoxification genes ͉ in situ hybridization ͉ insecticide resistance ͉ multigene family ͉ RNAi

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mentioning

confidence: 82%

Section: Temporal Expression Differences Of P450 Between Embryos Andmentioning

confidence: 99%

Section: Temporal Expression Differences Of P450 Between Embryos Andmentioning

confidence: 99%

mentioning

confidence: 99%

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Characterization of Drosophila melanogaster cytochrome P450 genes

Chung

Sztal

Pasricha

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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255

235

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“…Colony specimens for expression analyses were prepared in Table 1 exactly the same manner as the field-caught test subjects except that they were exposed to control, rather than insecticide-treated papers. To date, sublethal exposure to insecticide is not thought to result in wide-scale gene induction (54)(55)(56). Nevertheless, as there is some evidence for short-lived induction (57), the mosquitoes are held for 24 h postexposure so that any genes that are differentially expressed between groups most likely reflect constitutive rather than induced changes.…”

Section: Methodsmentioning

confidence: 99%

Identification and validation of a gene causing cross-resistance between insecticide classes inAnopheles gambiaefrom Ghana

Mitchell

Stevenson

Müller

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

226

255

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In the last decade there have been marked reductions in malaria incidence in sub-Saharan Africa. Sustaining these reductions will rely upon insecticides to control the mosquito malaria vectors. We report that in the primary African malaria vector, Anopheles gambiae sensu stricto, a single enzyme, CYP6M2, confers resistance to two classes of insecticide. This is unique evidence in a disease vector of cross-resistance associated with a single metabolic gene that simultaneously reduces the efficacy of two of the four classes of insecticide routinely used for malaria control. The gene-expression profile of a highly DDT-resistant population of A. gambiae s.s. from Ghana was characterized using a unique whole-genome microarray. A number of genes were significantly overexpressed compared with two susceptible West African colonies, including genes from metabolic families previously linked to insecticide resistance. One of the most significantly overexpressed probe groups (false-discovery rate-adjusted P < 0.0001) belonged to the cytochrome P450 gene CYP6M2. This gene is associated with pyrethroid resistance in wild A. gambiae s.s. populations) and can metabolize both type I and type II pyrethroids in recombinant protein assays. Using in vitro assays we show that recombinant CYP6M2 is also capable of metabolizing the organochlorine insecticide DDT in the presence of solubilizing factor sodium cholate.

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New Developments in the Control of Human Lice

Clark¹

2018

Ectoparasites

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A comparison of Drosophila melanogaster detoxification gene induction responses for six insecticides, caffeine and phenobarbital

Cited by 152 publications

References 50 publications

Characterization of Drosophila melanogaster cytochrome P450 genes

Characterization of Drosophila melanogaster cytochrome P450 genes

Identification and validation of a gene causing cross-resistance between insecticide classes inAnopheles gambiaefrom Ghana

New Developments in the Control of Human Lice

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