A comparison of DNA damage induced by aflatoxin B1 in hepatocyte-like cells, their progenitor mesenchymal stem cells and CD34+ cells isolated from umbilical cord blood

Ghaderi, Masoumeh; Allameh, Abdolamir; Soleimani, Masoud; Rastegar, Hossein; Ahmadi-Ashtiani, Hamid Reza

doi:10.1016/j.mrgentox.2010.09.005

Cited by 14 publications

(9 citation statements)

References 34 publications

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“…25 In the study of Ghaderi and colleagues, mesenchymal stem cells used as a control group of comet assay exhibited a higher damage index from cell basal metabolism (until almost 40, arbitrary index) than the DI found in the present study. 26 The variation of DI values found in the control group also occurred in other studies. 27 In the work of Hackenberg and colleagues, the authors also show a variation of DI values in the control groups using mesenchymal stem cells.…”

Section: -10supporting

confidence: 80%

Bio-electrospraying of human mesenchymal stem cells: An alternative for tissue engineering

et al. 2013

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Bio-electrospraying (BES) is a technique used for the processing of cells and can be applied to tissue engineering. The association of BES with scaffold production techniques has been shown to be an interesting strategy for the production of biomaterials with cells homogeneously distributed in the entire structure. Various studies have evaluated the effects of BES on different cell types. However, until the present moment, no studies have evaluated the impact of BES time on mesenchymal stem cells (MSC). Therefore, the aim of this work was to standardise the different parameters of BES (voltage, flow rate, and distance of the needle from the collecting plate) in relation to cell viability and then to evaluate the impact of BES time in relation to viability, proliferation, DNA damage, maintenance of plasticity and the immunophenotypic profile of MSC. Using 15 kV voltage, 0.46 ml/h flow rate and 4 cm distance, it was possible to form a stable and continuous jet of BES without causing a significant reduction in cell viability. Time periods between 15 and 60 min of BES did not cause alterations of viability, proliferation, plasticity, and immunophenotypic profile of the MSC. Time periods above 30 min of BES resulted in DNA damage; however, the DNA was able to repair itself within five hours. These results indicate that bio-electrospraying is an adequate technique for processing MSC which can be safely applied to tissue engineering and regenerative medicine.

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Section: -10supporting

confidence: 80%

Bio-electrospraying of human mesenchymal stem cells: An alternative for tissue engineering

et al. 2013

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“…30 Likewise, more recently, we reported that the pattern of lipid and protein oxidation is increasing during the differentiation process of the MSCs derived from human umbilical cord blood. In this line, earlier we reported that the hepatocyte-like cells and their progenitor stem cells exhibit differential susceptibilities to DNA damage when treated with a chemical carcinogen (i.e.…”

Section: Discussionmentioning

confidence: 88%

“…30,31 Under the glutathione depletion, the progenitor stem cells could be sensitized to ROS levels, which is beyond the normal levels. However, production of excessive ROS in the cells depleted with cellular glutathione can be deleterious to the cells and may increase the risk of oxidative damage.…”

Section: Discussionmentioning

confidence: 99%

“…However, production of excessive ROS in the cells depleted with cellular glutathione can be deleterious to the cells and may increase the risk of oxidative damage. 30,31 Under the glutathione depletion, the progenitor stem cells could be sensitized to ROS levels, which is beyond the normal levels. The differentiation-dependent changes in oxidative stress factors and related defense mechanisms may be considered as important factors in the integrity and functions of the cell lineage obtained as a result of stem cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

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The metabolic function of hepatocytes differentiated from human mesenchymal stem cells is inversely related to cellular glutathione levels

Allameh

Ahmadi-Ashtiani

Aleagha

et al. 2013

Cell Biochemistry & Function

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Differentiation of mesenchymal stem cells (MSCs) to hepatocytes-like cells is associated with alteration in the level of reactive oxygen species (ROS) and antioxidant defense system. Here, we report the role of glutathione in the functions of hepatocytes derived from MSCs. The stem cells undergoing differentiation were treated with glutathione modifiers [buthionine sulfoxide (BSO) or N-acetyl cysteine (NAC)], and hepatocytes were collected on day 14 of differentiation and analysed for their biological and metabolic functions. Differentiation process has been performed in presence of glutathione modifiers viz. BSO and NAC. Depending on the level of cellular glutathione, the proliferation rate of MSCs was affected. Glutathione depletion by BSO resulted in increased levels of albumin and ROS in hepatocytes. Whereas, albumin and ROS were inhibited in cells treated with glutathione precursor (NAC). The metabolic function of hepatocytes was elevated in BSO-treated cells as judged by increased urea, transferrin, albumin, alanine transaminase and aspartate transaminase secretions in the media. However, the metabolic activity of the hepatocytes was inhibited when glutathione was increased by NAC. We conclude that the efficiency of metabolic function of hepatocytes is inversely related to the levels of cellular glutathione. These data may suggest a novel role of glutathione in regulation of metabolic function of hepatocytes.

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“…Mycotoxins are secondary metabolic substances resulting from various strains of filamentous fungi (Qing-Hua et al, 2012). Aflatoxin B 1 is one of the most abundant and toxic known mycotoxins, (Karami-Osboo et al, 2012) responsible for carcinogenicity, hepatotoxicity and mutagenicity (Ghaderi et al, 2010;Li et al, 2011), including multiple developmental diseases (Cilievici et al, 1980;Dietert et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

Flavonoid hesperidin protects neural crest cells from death caused by aflatoxin B₁

Nones

Trentin

2012

Cell Biology International

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The neural crest (NC) corresponds to a collection of multipotent and oligopotent progenitors endowed with both neural and mesenchymal potentials. The derivatives of the NC at trunk level include neurons and glial cells of the peripheral nervous system. Despite the well-known influence of aflatoxins on the development of cancer, the issue of whether they also influence NC cells has not been yet addressed. In the present work, we have investigated the effects of aflatoxin B(1) on quail NC cells and the concomitant effects of the flavonoid hesperidin associated with this mycotoxin. We show for the first time that aflatoxin B(1) decreases the viability and the total number of glial and neuronal cells/field, although their proportions in relation to the total number of cells were not altered. Therefore, aflatoxin has no effect on NC differentiation. However, this compound was able to reduce NC proliferation and NC survival. Furthermore, the co-administration of hesperidin, a well-known polyphenolic protector of cell death, partially prevented the effect of aflatoxin B(1) . Taken together, our results demonstrate that aflatoxin B(1) is toxic to NC cells, an effect partially prevented by the flavonoid hesperidin. This study may contribute to the understanding of the effects of these compounds during early embryonic development and offer potentially more assertive diets and treatments for pregnant animals.

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A comparison of DNA damage induced by aflatoxin B1 in hepatocyte-like cells, their progenitor mesenchymal stem cells and CD34+ cells isolated from umbilical cord blood

Cited by 14 publications

References 34 publications

Bio-electrospraying of human mesenchymal stem cells: An alternative for tissue engineering

Bio-electrospraying of human mesenchymal stem cells: An alternative for tissue engineering

The metabolic function of hepatocytes differentiated from human mesenchymal stem cells is inversely related to cellular glutathione levels

Flavonoid hesperidin protects neural crest cells from death caused by aflatoxin B₁

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A comparison of DNA damage induced by aflatoxin B1 in hepatocyte-like cells, their progenitor mesenchymal stem cells and CD34+ cells isolated from umbilical cord blood

Cited by 14 publications

References 34 publications

Bio-electrospraying of human mesenchymal stem cells: An alternative for tissue engineering

Bio-electrospraying of human mesenchymal stem cells: An alternative for tissue engineering

The metabolic function of hepatocytes differentiated from human mesenchymal stem cells is inversely related to cellular glutathione levels

Flavonoid hesperidin protects neural crest cells from death caused by aflatoxin B1

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Flavonoid hesperidin protects neural crest cells from death caused by aflatoxin B₁