A comparison of different experimental configurations in pulsed laser induced molecular fluorescence

Richardson, J. H.; George, Steven M.

doi:10.1021/ac50026a019

Cited by 23 publications

(9 citation statements)

References 47 publications

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“…Ultratrace analysis of fluorescing molecules has made great progress by the use of laser sources for excitation. The unique properties of the nitrogen-laser-pumped dye laser can give rise to substantial gains in improving detection limits (1,2). The previously reported detection limits of fluorescein with a laser source and a monochromator (3)(4)(5) were in the order of 30-100 ppt (ppt = 10"12) and are approximately identical to that of a conventional fluorescence spectrophotometer.…”

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confidence: 89%

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Laser fluorometry of fluorescein and riboflavin

Ishibashi¹,

Ogawa²,

Imasaka³

et al. 1979

Anal. Chem.

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confidence: 89%

“…Figure 1. Fluorescence spectrum of fluorescein (pH 13, 5 X 10™11 M)(1) and background spectrum of solvent water(2). Excitation source:…”

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confidence: 99%

Laser fluorometry of fluorescein and riboflavin

Ishibashi¹,

Ogawa²,

Imasaka³

et al. 1979

Anal. Chem.

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“…Fluorescamine is known to react almost instantaneously with primary and secondary amines to give fluorescent pyrrolinones (FI) (1, 2) and nonfluorescent aminoenones (FII) (3,4), respectively (Scheme I). Fluorescamine itself is rapidly hydrolyzed to nonfluorescent products under reaction conditions (1).…”

Section: Fluorometric Determination Of Secondary Amines Based On Thei...mentioning

confidence: 99%

“…Nitrogen lasers and nitrogen laser pumped dye lasers have found wide-spread appications as short intense light sources for an ultratrace analysis of organic molecules (1)(2)(3)(4). This short pulse is essential for the application of lasers for time-resolved fluorometry.…”

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confidence: 99%

High repetition rate atmospheric pressure nitrogen laser for lifetime measurements

Imasaka¹,

Ishibashi²

1980

Anal. Chem.

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Consideration must be given to the instrument's portability if it is to be used for field studies utilizing a mobile laboratory. In this laboratory, Perkin-Elmer has proven reasonably portable. The Tracer is physically the smallest of the three but exhibits some operating difficulties in the best of laboratory conditions. The Varían is designed primarily as a laboratory research instrument but presumably is rugged enough to be used for field studies.Conclusions and Recommendations. It is evident from the data presented that actual experimental parameters may vary from the manufacturer's specifications as a result of laboratory conditions or differences in individual instruments. Therefore, it should be standard practice to characterize each FPD before placing any confidence in resulting data.The choice of an FPD system should be based upon careful consideration of individual laboratory needs and budgetary constraints. To select the instrument with the lowest detection limit would be unwise if the linear range does not extend to the concentration levels pertinent to a particular application. Additionally, instrumental options, such as other detectors, injection systems, and capillary accessories should be considered before purchasing.

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“…Comparisons to conventional luminescence have shown sensitivity improvements using laser excitation (4, 5). The feasibility and high sensitivity of the technique have been demonstrated in the analysis of rhodamine (5), aflatoxins (6), polynuclear aromatic hydrocarbons (7)(8)(9), phosphorimetric analysis of drugs (8,9), vitamins of the B complex (10,11), fluorescein (4,11), and several coumarin analogues (12). The improvements in sensitivity suggest the possibility of analyzing drugs in blood and plasma in the picogram (10"12 g) to femtogram (10~15 g) range required to quantitate high potency drugs administered at very low doses.…”

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confidence: 99%