A comparison of cytoplasmic and nuclear estradiol and progesterone receptors in human fallopian tube and endometrial tissue

Pollow, K.; Inthraphuvasak, J.; Manz, B.; Grill, H. J.; Pollow, Barbara

doi:10.1016/s0015-0282(16)45860-7

Cited by 54 publications

(30 citation statements)

References 13 publications

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“…In addition, a decrease in TRE2 levels was found along the longitudinal axis of the uterus. Similar findings were reported for the cytosolic RE2 by Tsibris et al [14] and Kauppilla et al [15], These findings (1 and 2) were statistically significant despite a large interindividual range of values, also previously described [3,16,17], and we therefore suggest that they are neither an artifact nor method-depen dent. A possibility exists, however, that they are associated with tissue cellularity differ ences, i.e.…”

Section: Discussionsupporting

confidence: 92%

“…The highest TRE: levels in the normal cy cling endometrium were measured in the mid cycle (days 13-16 of an ideal 28-day cycle) and the lowest in the secretory phase (days [17][18][19][20][21][22]. Due to large interindividual differences these changes did not reach statistical significance; TRE2 levels in normal postmenopausal endo metrium (atrophic or inactive) were low (table I).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Distribution of Total Estradiol Receptor Levels in Various Segments and Tissues of the Normal and Pathological Human Uterus

Gorodeski

Bahary²,

Lunenfeld³

et al. 1988

Gynecol Obstet Invest

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Estradiol receptor (RE₂) levels were measured in normal (n = 22) and pathological (n = 14) human endometria and in various segments and tissues of the same uteri (n = 8). The highest total estradiol levels (TRE₂) were found in midcycle normal cyclic endometria and the lowest in the secretory phase of normal cyclic endometria and in normal postmeno-pausal endometria (the TRE₂ levels in the latter two groups were within similar ranges). The highest nuclear RE₂ levels were found in proliferative-phase normal cyclic endometria. TRE₂ levels in pathological postmenopausal endometria were high, in the range of those found in proliferative normal cyclic endometria, but the nuclear RE₂ levels were low as compared with those in normal proliferative endometria. Examination of the distribution of the TRE₂ levels in various segments and tissues of normal proliferative uteri revealed similar trends in all cases as follows: the highest levels were measured in the functional endometrium (fundus) and the lowest in the myometrium (fundus). Basal endometrial TRE₂ levels decreased along the longitudinal axis from fundus to isthmus. In 4 cases, TRE₂ levels in leiomyoma tissues were higher than those in the corresponding myometrium. These results indicate the possibility of a differing regulating mechanism of the RE₂ in various segments or tissues of the human uterus.

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Section: Discussionsupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Distribution of Total Estradiol Receptor Levels in Various Segments and Tissues of the Normal and Pathological Human Uterus

Gorodeski

Bahary²,

Lunenfeld³

et al. 1988

Gynecol Obstet Invest

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“…The relationship between hormone receptor-expressing cells and tubal function is a fundamental issue in fallopian tube biology, and earlier studies (43,44,60,61) have demonstrated that specific binding sites for E 2 can be observed using the ligand-binding assay in human fallopian tubes. This E 2 -binding activity is probably due to both ER subtypes.…”

mentioning

confidence: 99%

Ciliated epithelial-specific and regional-specific expression and regulation of the estrogen receptor-β2 in the fallopian tubes of immature rats: a possible mechanism for estrogen-mediated transport process in vivo

Shao¹,

Weijdegård²,

Fernández-Rodrı́guez³

et al. 2007

American Journal of Physiology-Endocrinology and Metabolism

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eral ER␤ isoforms have been identified in human and rodent tissues, but it is unclear whether each isoform has distinctly different cellular targeting characteristics and physiological functions. We have investigated the intracellular localization and regulatory patterns for ER␤ isoforms in rat fallopian tubes. Western blot analysis reveals that two ER␤ isoforms corresponding to ER␤1 and ER␤2 are expressed in rat fallopian tubes. However, ER␤2 is the predominant form of ER␤ in this tissue. High-resolution confocal imaging and immunohistochemical analysis provide ample evidence that ER␤ expression is limited almost exclusively to the ciliated epithelial cells, in contrast to ER␣, which is widely distributed. Furthermore, within the ciliated epithelial cells, ER␤ is colocalized with ␤-tubulin IV at stem portion of the cilia. We show that ER␤2 protein expression is tightly regulated by E 2 or DPN in a time-dependent manner without changes in ER␤1 expression. These estrogenic effects are inhibited by an ER antagonist, ICI 182,780. In addition, significant alteration of ER␤ immunoreactivity is detected only histologically in the ampullary region. Since the cilia are considered an essential determinant of tubal transport, we further demonstrate that E 2-or DPN-induced ER␤2 activation is associated with alterations in tubal protein expression crucial for the regulation of calcium-dependent ciliary beating. Given the coordinated regulation and interaction of ER and progesterone receptor in the cilia, we hypothesize that tubal ER␤2 may facilitate the estrogen-mediated transport process by processing protein-protein interaction under physiological and/or pathological conditions. We show for the first time that a previously unrecognized localization of ER␤ isoform in rat fallopian tubes can combine with estrogen to individually control the expression of ER ␤-isoforms in normal target tissues. estrogen receptor-␤ isoform; ciliated epithelial cells; ampulla

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“…A previous report showed that the in vitro addition of PRG decreased CBF in a human Fallopian tube explant (fimbrial region) by 40-50% (13). Moreover, both βE 2 and PRG receptors have been found in the epithelia of the Fallopian tubes, and the expressions of these two receptors vary in relation to the phase of the ovarian cycle (3,16,18,24). These observations indicate that CBF in the fimbria is regulated by βE 2 and PRG.…”

Section: Animals and Treatmentsmentioning

confidence: 81%

The regulation of ciliary beat frequency by ovarian steroids in the guinea pig Fallopian tube: interactions between oestradiol and progesterone

et al. 2011

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Ciliary beat frequency (CBF) was measured in slice preparations of the Fallopian tube fimbria, using videomicroscopy with a high-speed (500 Hz) camera in guinea pigs that were treated with β-oestradiol benzoate (βE 2 B) and medroxy progesterone (mPRG). In non-ovulating guinea pigs at 4 weeks of age, the CBF of the fimbria was high (17.8 Hz). In sexually mature guinea pigs (12-16 weeks of age) with constant ovulation, the CBF varied from 12 Hz to 16 Hz. The in vivo administration of both ICI-182,780 (a blocker of βE 2 receptors) and mifepristone (a blocker of PRG receptors) induced high CBF (17.4 Hz). The administration of βE 2 B at a low (3.2 mg/kg/day) or high (32 mg/kg/day) dose decreased the CBF to 14.5 Hz or 11 Hz, respectively. ICI-182,780 abolished the βE 2 B-induced changes in CBF and decreased CBF to 12 Hz. The administration of mPRG (6.4 mg/kg/day) decreased CBF to 12.5 Hz. Mifepristone abolished this mPRG-induced decrease in CBF and maintained the CBF at 15 Hz. However, administering both βE 2 B and mPRG increased CBF to 17.5 Hz, suggesting that βE 2 B inhibits mPRG actions and vice versa. To confirm the interactions between βE 2 B and mPRG, we administered both βE 2 B and mPRG to guinea pigs that were pretreated for 1.5 days with either mPRG (6.4 mg/kg/day) or βE 2 B (3.2 mg/kg/day). Prior treatment with βE 2 B or mPRG prevented the increase in CBF that was otherwise by βE 2 B plus mPRG, and maintained the CBF at 14.5 Hz or 13 Hz, respectively. The administration of βE 2 B plus mPRG still induced the expression of PRG receptors, indicating that the highest CBF is not the result of no expression of the receptors. In the beating cilia of the fimbria, the signals that are activated by the βE 2 and PRG receptors are proposed to antagonize each other in regulating the frequency.

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A comparison of cytoplasmic and nuclear estradiol and progesterone receptors in human fallopian tube and endometrial tissue

Cited by 54 publications

References 13 publications

Distribution of Total Estradiol Receptor Levels in Various Segments and Tissues of the Normal and Pathological Human Uterus

Distribution of Total Estradiol Receptor Levels in Various Segments and Tissues of the Normal and Pathological Human Uterus

Ciliated epithelial-specific and regional-specific expression and regulation of the estrogen receptor-β2 in the fallopian tubes of immature rats: a possible mechanism for estrogen-mediated transport process in vivo

The regulation of ciliary beat frequency by ovarian steroids in the guinea pig Fallopian tube: interactions between oestradiol and progesterone

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