A Comparison of Cytochrome P4501A (CYP1A) mRNA Inducibility in Four Species of Atlantic Coast Anadromous Fishes

Wirgin, Isaac; Konkle, Blake; Pedersen, Mark; Grunwald, Cheryl; Williams, Jim; Courtenay, Simon C.

doi:10.2307/1352307

Cited by 11 publications

(5 citation statements)

References 49 publications

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“…Cytochrome P4501A can also be activated by other pathways and plays a strong role in steroid hormone biosynthesis, tryptophan and retinol metabolism (review in Monostory et al 2009). Induction of Cyp1a varies between fish species (Wirgin et al 1996;Willett et al 1997;Oris and Roberts 2007), so that a specific evaluation needs to be performed before using this biomarker on a sentinel species in environmental monitoring programs. In this study, we thus focus on both Cyp1a gene expression and protein content in order to provide a combined biomarker specific for the striped bass Morone saxatilis.…”

Section: Introductionmentioning

confidence: 99%

Cytochrome P4501A mRNA and protein induction in striped bass (Morone saxatilis)

Durieux

Connon

Werner

et al. 2012

Fish Physiol Biochem

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The striped bass (Morone saxatilis) supports a valuable recreational fishery and is among the most important piscivorous fish of the San Francisco Estuary. This species has suffered a significant decline in numbers over the past decades, and there is indication that contaminants are important contributors. Polycyclic aromatic hydrocarbons (PAHs) and polyhalogenated aromatic hydrocarbons (PHAHs) including PCBs and dioxins are widespread in the estuary, they typically bioaccumulate through trophic levels, reaching highest levels in top predators and are known to affect the fish health and development. The aim of this study was to investigate the dynamics of cytochrome P4501A (Cyp1a) induction simultaneously at different levels of biological organization (RNA transcription and protein synthesis) as a biomarker of exposure to PAHs and PHAHs. We utilized β-naphthoflavone (BNF) as a model PAH to induce Cyp1a responses in juvenile striped bass in both dose-response and time-response assessments and determined Cyp1a mRNA and protein levels. Significant responses were measured in both systems at 10 mg ΒΝF kg⁻¹, a concentration used for time-response studies. Messenger RNA levels peaked at 6 h post-injection, while protein levels increased progressively with time, significantly peaking at 96 h post-injection; both remaining elevated throughout the duration of the test (8 days). Our data suggest that rapid induction of gene transcription following exposure and subsequent cumulative protein synthesis could provide a useful means of identifying temporal variants in exposure to Cyp1a inducers in Morone saxatilis. The potential application of this combined Cyp1a gene and protein biomarker in this species for field studies is discussed.

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Section: Introductionmentioning

confidence: 99%

Cytochrome P4501A mRNA and protein induction in striped bass (Morone saxatilis)

Durieux

Connon

Werner

et al. 2012

Fish Physiol Biochem

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“…CYP1A was detected in both fish species at all examined localities. 21 Thus, the Atlantic tomcod exhibited significant induction of CYPlA mRNA after exposure to chemicals (97-fold) and changes in the en-vironment (34-fold), whereas in the smooth flounder, a considerably lower level of CYP1A induction was observed in response to chemical exposure (14-fold) but no change in expression after exposure to adjustments in the environment. 2 and Table I).…”

Section: The Effects Of Exposure To Pahs and Pcbs On Cyp1a Expressionmentioning

confidence: 90%

“…The highest degree of induction was observed in both fish species that were caught in the port Bar ( Fig. 21 In hog chokers and striped bass, very low levels of CYP1A gene induction were detected. The results presented in Fig.…”

Section: The Effects Of Exposure To Pahs and Pcbs On Cyp1a Expressionmentioning

confidence: 92%

Oxidative dehydrogenation of isobutane over supported V-Mo mixed oxides

Mitran¹,

Marcu²,

Urdă³

et al. 2010

JSCS

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The enzyme CYP1A is an established biomarker of fish exposure to polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs). The metallothioneins (MT), a family of Cys-rich proteins, bind a wide range of metals and participate in their metabolism. The aim of the study was to examine the correlation between CYP1A and MT expression in commercially important fish species Mullus barbatus and Merluccius merluccius and contaminants (PAHs, PCBs, toxic metals) in seawater and sediment from three localities with different level of contamination in the Adriatic Sea in winter, i.e., Platamuni, Valdanos and the port of Bar. The relative concentration of CYP1A was the highest in both fish species from Bar. Increased concentrations of PCBs in the seawater were observed only in Bar. A species-specific higher increase in the protein concentration of CYP1A was observed in Mullus barbatus compared to Merluccius merluccius. The levels of MT were the highest in Merluccius merluccius from Bar and in Mullus barbatus from Valdanos. The induction of MT correlated with the elevated concentrations of Cu and Pb determined by chemical analysis of the seawater from Bar and Valdanos, respectively. According to the chemical analysis of the seawater and the biological response of the fish, the Platamuni locality exhibited the lowest level of contamination. EXPERIMENTAL AnimalsSpecimens of Mullus barbatus and Merluccius merluccius were collected by trawling at Platamuni, Valdanos and the port of Bar (Fig. 1). The investigations took place in winter (25 th February, 2009). At least seven (and up to nine) individual fish of one species were pooled. The fish were killed immediately by spinosectomy according to standard animal care regulations. The hepatopancreas was quickly removed, washed in ice-cold 0.15 M NaCl and frozen in liquid nitrogen. Individuals of the same size were selected to ensure uniformity of samples.

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“…Virgin et al showed that there are large differences in CYPlA mRNA induction between species. 21 Thus, the Atlantic tomcod exhibited significant induction of CYPlA mRNA after exposure to chemicals (97-fold) and changes in the en-vironment (34-fold), whereas in the smooth flounder, a considerably lower level of CYP1A induction was observed in response to chemical exposure (14-fold) but no change in expression after exposure to adjustments in the environment. 21 In hog chokers and striped bass, very low levels of CYP1A gene induction were detected.…”

Section: The Effects Of Exposure To Pahs and Pcbs On Cyp1a Expressionmentioning

confidence: 90%

“…21 Thus, the Atlantic tomcod exhibited significant induction of CYPlA mRNA after exposure to chemicals (97-fold) and changes in the en-vironment (34-fold), whereas in the smooth flounder, a considerably lower level of CYP1A induction was observed in response to chemical exposure (14-fold) but no change in expression after exposure to adjustments in the environment. 21 In hog chokers and striped bass, very low levels of CYP1A gene induction were detected. The degree of CYPlA mRNA inductibility above the basal level differs significantly among fish taxa, most likely as a result of differences in the regulation of gene expression.…”

Section: The Effects Of Exposure To Pahs and Pcbs On Cyp1a Expressionmentioning

confidence: 90%

CYP1A and metallothionein expression in the hepatopancreas of Merluccius merluccius and Mullus barbatus from the Adriatic sea

Mihailović¹,

Petrović²,

Grdović³

et al. 2010

JSCS

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The enzyme CYP1A is an established biomarker polychlorinated biphenyls of fish exposure to polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs). The metallothioneins (MT), a family of Cys-rich proteins, bind a wide range of metals and participate in their metabolism. The aim of the study was to examine the correlation between CYP1A and MT expression in commercially important fish species Mullus barbatus and Merluccius merluccius and contaminants (PAHs, PCBs, toxic metals) in seawater and sediment from three localities with different level of contamination in the Adriatic Sea in winter, i.e., Platamuni, Valdanos and the port of Bar. The relative concentration of CYP1A was the highest in both fish species from Bar. Increased concentrations of PCBs in the seawater were observed only in Bar. A species-specific higher increase in the protein concentration of CYP1A was observed in Mullus barbatus compared to Merluccius merluccius. The levels of MT were the highest in Merluccius merluccius from Bar and in Mullus barbatus from Valdanos. The induction of MT correlated with the elevated concentrations of Cu and Pb determined by chemical analysis of the seawater from Bar and Valdanos, respectively. According to the chemical analysis of the seawater and the biological response of the fish, the Platamuni locality exhibited the lowest level of contamination.

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A Comparison of Cytochrome P4501A (CYP1A) mRNA Inducibility in Four Species of Atlantic Coast Anadromous Fishes

Cited by 11 publications

References 49 publications

Cytochrome P4501A mRNA and protein induction in striped bass (Morone saxatilis)

Cytochrome P4501A mRNA and protein induction in striped bass (Morone saxatilis)

Oxidative dehydrogenation of isobutane over supported V-Mo mixed oxides

CYP1A and metallothionein expression in the hepatopancreas of Merluccius merluccius and Mullus barbatus from the Adriatic sea

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