A Comparison of Coding Sequence and Cytogenetic Localization of the Myostatin Gene in the Dog, Red Fox, Arctic Fox and Chinese Raccoon Dog

Grzes, Maria; Nowacka‐Woszuk, Joanna; Szczerbal, Izabela; Czerwińśka, Joanna; Gracz, Joanna; Świtoński, M.

doi:10.1159/000245917

Cited by 7 publications

(9 citation statements)

References 56 publications

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“…A similar comparison, fo-cused on a 131-bp fragment of the IGF1 gene (a part of exon 2), revealed only 3 single nucleotide substitutions and the highest nucleotide similarity between the dog and Chinese raccoon dog [Klukowska et at., 2003]. Recently, Grzes et al [2009] analyzed the sequence of the MSTN gene (all exons and fragments of 5 -and 3 -flanking regions). The interspecies differences were identified in exons 1 and 2.…”

Section: Comparison Of Gene Sequencesmentioning

confidence: 99%

Comparative Genomics of 3 Farm Canids in Relation to the Dog

Świtoński¹,

Szczerbal²,

Nowacka‐Woszuk³

2009

Cytogenet Genome Res

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There are 3 canids besides the dog (Canis familiaris): the red fox (Vulpes vulpes), arctic fox (Alopex lagopus) and Chinese raccoon dog (Nyctereutes procyonoides procyonoides), which have been extensively studied with the use of cytogenetic and molecular genetics techniques. These 3 species are considered as farm fur-bearing animals. In addition, they are also useful models in comparative genomic studies of the canids. In this review genome organization, karyotype evolution, comparative marker maps, DNA polymorphism and similarity of selected gene sequences of the 3 farm species are discussed in relation to the dog. Also the nature and variability of the B chromosomes, present in the red fox and the Chinese raccoon dog, were considered. These comparative analyses showed that among the studied canids the Chinese raccoon dog is phylogenetically the closest species to the dog. On the other hand, the most advanced linkage and cytogenetic marker maps of the red fox genome facilitate genome scanning studies with the aim to search for chromosome locations of QTL regions for behavior and production traits.

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Section: Comparison Of Gene Sequencesmentioning

confidence: 99%

Comparative Genomics of 3 Farm Canids in Relation to the Dog

Świtoński¹,

Szczerbal²,

Nowacka‐Woszuk³

2009

Cytogenet Genome Res

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“…Early phylogenies represented species divergence from a common ancestor based on morphological and geographical features. More recently, phylogenetic trees have been constructed by DNA sequence analysis of various nuclear-and mitochondrialencoded loci (Wayne 1993;Wayne and Ostrander 1999;Zrzavy and Ricankova 2004;Bardeleben et al 2005;Lindblad-Toh et al 2005;Grzes et al 2009;Schröder et al 2009), but these have led to a number of contradictory phylogenetic placements. Conventional chromosome banding and molecular cytogenetic studies have enabled the construction of alternative phylogenetic trees for the Canidae, taking into account gross karyotypic changes that occurred during speciation (Wayne et al 1987a(Wayne et al , b, 1997Nash et al 2001;Graphodatsky et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Anchoring the dog to its relatives reveals new evolutionary breakpoints across 11 species of the Canidae and provides new clues for the role of B chromosomes

et al. 2011

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The emergence of genome-integrated molecular cytogenetic resources allows for comprehensive comparative analysis of gross karyotype architecture across related species. The identification of evolutionarily conserved chromosome segment (ECCS) boundaries provides deeper insight into the process of chromosome evolution associated with speciation. We evaluated the genome-wide distribution and relative orientation of ECCSs in three wild canid species with diverse karyotypes (red fox, Chinese raccoon dog, and gray fox). Chromosome-specific panels of dog genome-integrated bacterial artificial chromosome (BAC) clones spaced at ∼10-Mb intervals were used in fluorescence in situ hybridization analysis to construct integrated physical genome maps of these three species. Conserved evolutionary breakpoint regions (EBRs) shared between their karyotypes were refined across these and eight additional wild canid species using targeted BAC panels spaced at ∼1-Mb intervals. Our findings suggest that the EBRs associated with speciation in the Canidae are compatible with recent phylogenetic groupings and provide evidence that these breakpoints are also recurrently associated with spontaneous canine cancers. We identified several regions of domestic dog sequence that share homology with canid B chromosomes, including additional cancer-associated genes, suggesting that these supernumerary elements may represent more than inert passengers within the cell. We propose that the complex karyotype rearrangements associated with speciation of the Canidae reflect unstable chromosome regions described by the fragile breakage model.

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“…Three species studies have already been conducted on several genes involved in muscularity-MSTN (Grzes et al, 2009) MC4R GENE OF THE CHINESE RACCOON DOG AND ARCTIC FOX (Skorczyk et al, 2007;Grzes et al, 2011;Nowacka-Woszuk et al, 2011), and sex determination-SRY, SOX9, AR, and AMH . In these genes, numerous polymorphisms were described, but only a polymorphism of the MC3R was associated with the body weight of red foxes .…”

Section: Discussionmentioning

confidence: 99%

A Comparative Analysis ofMC4RGene Sequence, Polymorphism, and Chromosomal Localization in Chinese Raccoon Dog and Arctic Fox

Skorczyk

Flisikowski

Świtoński

2012

DNA and Cell Biology

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Numerous mutations of the human melanocortin receptor type 4 (MC4R) gene are responsible for monogenic obesity, and some of them appear to be associated with predisposition or resistance to polygenic obesity. Thus, this gene is considered a functional candidate for fat tissue accumulation and body weight in domestic mammals. The aim of the study was comparative analysis of chromosome localization, nucleotide sequence, and polymorphism of the MC4R gene in two farmed species of the Canidae family, namely the Chinese raccoon dog (Nycterutes procyonoides procyonoides) and the arctic fox (Alopex lagopus). The whole coding sequence, including fragments of 3'UTR and 5'UTR, shows 89% similarity between the arctic fox (1276 bp) and Chinese raccoon dog (1213 bp). Altogether, 30 farmed Chinese raccoon dogs and 30 farmed arctic foxes were searched for polymorphisms. In the Chinese raccoon dog, only one silent substitution in the coding sequence was identified; whereas in the arctic fox, four InDels and two single-nucleotide polymorphisms (SNPs) in the 5'UTR and six silent SNPs in the exon were found. The studied gene was mapped by FISH to the Chinese raccoon dog chromosome 9 (NPP9q1.2) and arctic fox chromosome 24 (ALA24q1.2-1.3). The obtained results are discussed in terms of genome evolution of species belonging to the family Canidae and their potential use in animal breeding.

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A Comparison of Coding Sequence and Cytogenetic Localization of the Myostatin Gene in the Dog, Red Fox, Arctic Fox and Chinese Raccoon Dog

Cited by 7 publications

References 56 publications

Comparative Genomics of 3 Farm Canids in Relation to the Dog

Comparative Genomics of 3 Farm Canids in Relation to the Dog

Anchoring the dog to its relatives reveals new evolutionary breakpoints across 11 species of the Canidae and provides new clues for the role of B chromosomes

A Comparative Analysis ofMC4RGene Sequence, Polymorphism, and Chromosomal Localization in Chinese Raccoon Dog and Arctic Fox

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