A Comparison of Characterization and Its Actions on Immunocompetent Cells of Polysaccharides from Sijunzi Decoction

Abstract

Sijunzi decoction (SJZD) is a classic recipe in Traditional Chinese Medicine (TCM) with strong immune-enhancement activity. To further understand the characterization and immunomodulatory effect of polysaccharides from SJZD, the monosaccharide compositions of crude polysaccharide (SJZDP), polysaccharide fraction (S-3), and homogeneous polysaccharide (S-3-AG) from SJZD were compared by GC analysis, as well as their immunomodulatory effects on Peyer’s patch cells, splenocytes, and macrophages which are related to intestinal immunity, specific immunity, and nonspecific immunity. The results showed that S-3-AG mainly contained Ara with a proportion of 38.9%, while Glc accounted for the largest proportion in S-3 (55.6%) and SJZDP (87.6%). The SJZDP, S-3, and S-3-AG all showed strong capability to stimulate Peyer’s patch cells to proliferate and produce IgA and promoted the proliferation and IFN-γ production of splenocytes and increased the NO production and TNF-α production of macrophages. However, S-3 and S-3-AG were able to stimulate splenocytes to secret IL-4, SJZDP had no effect on IL-4 production of splenocytes in the tested concentrations. In addition, S-3 could stimulate the phagocytic activity of macrophages, and S-3-AG restrained the proliferation of macrophages at the concentration of 50–200 µg/mL. These results suggested that SJZDP, S-3, and S-3-AG might have different immunomodulatory effects on intestinal immunity, specific immunity, and nonspecific immunity due to their different monosaccharide compositions. It will provide references for the material basis and mechanism of SJZD immunomodulation activity.

Clinical Efficacy and Gut Microbiota Regulating-Related Effect of Si-Jun-Zi Decoction in Postoperative Non-Small Cell Lung Cancer Patients: A Prospective Observational Study

“… 49 The extraction of different monosaccharide compositions from SJZD has noticeable effects on intestinal immunity, specific immunity, and nonspecific immunity. 50 It has the capability to augment the adaptive immune response, regulate the intestinal immune system, and facilitate the restoration of gastrointestinal function, which is through the reduction of CD3 + T cells and CD8 + T cells, and the elevation of CD4 + T cells. 51 Furthermore, SJZD has demonstrated efficacy in mitigating inflammation by enhancing intestinal local immunity.…”

“… 57 Previous studies have consistently shown that SJZD enhanced the diversity and constitution of the gut microbiota, while also exerting an immunomodulatory influence by regulating the gut microbiota. 26 , 50 , 58 …”

Comparative Study on the Mechanism of Macrophage Activation Induced by Polysaccharides from Fresh and Dried Longan

“…Previous studies have indicated that longan polysaccharides can reach the intestine in an undigested form without significant changes in immunomodulatory activity [ 6 ]. Longan polysaccharides may exert immunomodulatory activity by activating macrophages within the intestinal immune system [ 23 ]. The present study showed that polysaccharides extracted from dried and fresh longan differentially promote phagocytosis and NO, TNF-α, and IL-6 secretion.…”

Exploring the Potential Molecular Mechanism of Sijunzi Decoction in the Treatment of Non-Segmental Vitiligo Based on Network Pharmacology and Molecular Docking

“… 6 Crude polysaccharide (SJZDP), polysaccharide fraction (S-3) and homogeneous polysaccharide (S-3-AG) separated from SJZD have immune-enhancing effects, and may have different immune regulation effects on intestinal immunity, specific immunity and non-specific immunity due to their different monosaccharide compositions. 7 Mechanism of action study has shown that SJZD can enhance the immune function of mice by regulating the janus kinase (JAK)-signal transducer and activator of transcription (STAT) signaling pathway. 8 SJZD may also help reduce intestinal injury after burns and prevent intestinal bacterial translocation.…”