Out of 70 S. aureus strains isolated from the anterior nares of horses, 48 (69 per cent) belonged to the E biotype. Approximately one third of these isolates were typed with factor sera, the 6 (35 per cent) that were typable showing 5 different patterns. All strains but one were non‐typable with the basic sets of phages for typing human and bovine staphylococci even at RTD x 100. Without any exception the equine staphylococci of the E biotype contained polysaccharide Aα. Sixteen biochemically different strains belonged to the biotype A, B or C. A number of different serological patterns and phage patterns were found in these strains. In contrast to the typical horse‐adapted staphylococci of the E biotype they had polysaccharide Aβ. The remaiinng 6 cultures could not be classified.