A comparison of an amoeba, Vahlkampfia patuxent, with tissue‐culture cells

Hogue, Mary Jane

doi:10.1002/jez.1400350102

Cited by 8 publications

(2 citation statements)

References 3 publications

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“…If these organisms are transferred to fresh culture media there is no growth or multiplication, showing that they are dead. Here again motion and locomotion can be taken as a criterion of life, as I (17) have already shown to be the case for some other organisms.…”

Section: Effect Of Different Media--there Was Little Morphological Dmentioning

confidence: 96%

Spirochæta Eurygyrata

Hogue

1922

Journal of Experimental Medicine

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1. Pure lines of Spirochœta eurygyrata were isolated and cultured. 2. They lived longest in a medium which was made up of sodium chloride and pig serum water. 3. They stained readily with iron-hematoxylin, Giemsa's stain, Cross' stain, and carbolfuchsin. 4. They did not stain with the vital dyes used. 5. They divide by transverse division. 6. Transverse bars, which are seen in stained spirochetes and in dead ones with dark-field illumination, are considered coagulated protoplasm, due to death. 7. Coccoid bodies were not seen in living individuals nor were they seen free in the culture medium. 8. Cats were fed cultures of Spirochœta eurygyrata but did not become infected with them. 9. Spirochœta eurygyrata is not of frequent occurrence in this part of the United States.

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Section: Effect Of Different Media--there Was Little Morphological Dmentioning

confidence: 96%

Spirochæta Eurygyrata

Hogue

1922

Journal of Experimental Medicine

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“…Although they are relatively non-toxlc (Hogue, 1922), these methods of supravital staining are open to serious question unless very carefully controlled. Since the cells are slowly dying, not through any toxic action of the stain but on account of traumatism and unfavorable environmental conditions, it is important not to prolong observations more than 30 minutes after the cells are removed from the cornea; certainly the moment that they show evidences of shrinkage, or of the adsorption of fluid with the release of molecular bombardment causing brownian movement, or of coloration of the nuclei, the preparations should be discarded.…”

Section: Observationsmentioning

confidence: 99%

The Supravital Staining of Vaccine Bodies

Cowdry

1922

Journal of Experimental Medicine

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Vaccine bodies in living corneal cells may be specifically stained by the addition of a small quantity of brilliant cresyl blue 2 B to the physiological salt solution in which they are being observed. Their appearance by this method (Figs. 3 to 17) corresponds with that seen in fixed preparations (Figs. 22 to 42). Both lines of study reveal the existence of traces of similar material in unvaccinated corneal cells. As this increases in amount during the reaction, it behaves like an integral, cytoplasmic constituent of fluid consistency and shows no evidence of being endowed with any measure of independent vitality. The low grade of structural differentiation which it does exhibit, in living cells as well as in fixed tissues, is not suggestive of the presence within it of independent microorganisms. The material differs radically in its morphology and microchemical reactions from the granules observed by MacCallum and Oppenheimer in vaccine lymph.

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The reaction of tissue‐culture cells to barium (x‐ray) sulphate

Hogue

1932

Anat. Rec.

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A comparison of an amoeba, Vahlkampfia patuxent, with tissue‐culture cells

Cited by 8 publications

References 3 publications

Spirochæta Eurygyrata

Spirochæta Eurygyrata

The Supravital Staining of Vaccine Bodies

The reaction of tissue‐culture cells to barium (x‐ray) sulphate

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