A Comparison in Guinea Pigs of Brucella Abortus S.19 Grown by Conventional and Continuous Methods

Pivnick, Hilliard; Crawley, J. F.

doi:10.1139/m62-055

Cited by 3 publications

(1 citation statement)

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“…A liquid medium for the preparation of vaccines for the treatment of human infection was described by Zaitseva (1961). Pivnick & Crawley (1962) found that Strain 19, grown by continuous culture, was as safe and immunogenic in guineapigs as the vaccine grown on solid medium.…”

Section: Vaccinationmentioning

confidence: 99%

Section E. Diseases of dairy cattle. Brucellosis

Morgan

1964

Journal of Dairy Research

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Those phages, with their propagating cultures, can be obtained from the FAO/WHO Brucellosis Centre, M.A.F.F. Central Veterinary Laboratory, Weybridge. 21 Dairy Res. 31 Reviews of the progress of dairy science Jones also showed that cultures of Br. melitensis isolated from cattle in Malta were not lysed by phage. Whereas the majority of cultures of Br. melitensis isolated from cattle, which had so far been studied, were lysed by phage, not all cultures of Br. melitensis isolated from cattle were lysed. Recently, Payne (personal communication) isolated Br. melitensis from human blood as well as from bovine milk samples from the supplying farm. All these cultures were lysed by phage (and, in the author's laboratory were also shown to have the oxidative metabolic pattern of Br. abortus). Therefore, cattle and humans can be infected with both the British and Mediterranean strains of Br. melitensis; this fact in no way invalidates the usefulness of phage typing of Brucella as suggested by Parnas & Burdzy (1963). Ulbrich & Weinhold (1962), using phage P i and F25 from South Africa, examined 143 strains of brucella and also found that cultures of Br. abortus were lysed, Br. suis were not lysed and some Br. melitensis were lysed and some were not. The position was considerably clarified by the work of Meyer (1961a.) (see also below under 'Classification of Brucella') who showed quite clearly that cultures with the oxidative metabolic pattern characteristic of Br. abortus, irrespective of their properties as determined by conventional tests, were lysed by phage type abortus Strain 3 at R.T.D. Morgan (1963) examined nearly 4000 brucella cultures using phage Tb and 4 Polish phages at R.T.D. and 10000 x R.T.D. All cultures of Br. abortus examined were lysed by all 5 phages at both dilutions. In addition, cultures with the oxidative metabolic pattern characteristic of Br. abortus, irrespective of their properties as determined by conventional typing methods, were lysed by both phage dilutions. Twenty-two cultures of Br. suis were not lysed by phage at R.T.D. but all showed lysis by phage at 10000 x R.T.D. Cultures of Br. melitensis and those with the oxidative metabolic pattern characteristic of Br. melitensis, irrespective of their properties as determined by conventional typing methods, were not lysed by phages at either dilution. All the 5 phages used displayed an identical host range; this close similarity was confirmed by neutralization tests with phage antiserum. Wundt (1962), using 8 Polish phages, found that all cultures of Br. abortus were lysed by all the phages, whereas cultures of Br. suis were not lysed. Of 17 cultures of Br. melitensis isolated from cattle in Northern Germany, 13 were lysed whereas of 29 cultures of Br. melitensis isolated in south-western Germany, only one was lysed. Miinter (1963) examined 133 cultures with 22 phages (from U.S.S.R., Poland and South Africa). All 22 phages had an identical host range. Cultures of Br. abortus and Br. suis showed complete lysis at 10000 x R.T.D. but, at R.T.D. onl...

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Section: Vaccinationmentioning

confidence: 99%