A comparison between the effects of three potential scar-reducing agents applied at a site of sciatic nerve repair

Ngeow, Wei Cheong; Atkins, Simon; Morgan, Claire R.; Metcalfe, Anthony D.; Boissonade, Fiona M.; Loescher, Alison R.; Robinson, P.P.

doi:10.1016/j.neuroscience.2011.02.054

Cited by 12 publications

(14 citation statements)

References 43 publications

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“…This present histomorphometric study did not reveal any difference between the repaired nerves treated with the scar‐reducing agent M6P or the PBS placebo. As the results of our previous electrophysiological studies have shown that M6P treatment can enhance nerve regeneration (Ngeow et al. 2011a,b), we hypothesised that we would see an increase in the number and/or size of the myelinated axons distal to the repair site.…”

Section: Discussionmentioning

confidence: 97%

“…The remaining solution (80 μL) was then injected between the nerve ends and into the muscle and soft tissues surrounding the repair, and the surgical site closed. A single dose of analgesic was administered (0.01 mL buprenorphine hydrochloride 0.3 mg mL −1 ; Vetergesic ® , Alstoe Animal Health, UK) subcutaneously, and the mice were allowed to recover for 6 weeks; this recovery period was chosen to be the same as used in our previous electrophysiological studies where M6P was shown to have enhanced regeneration (Ngeow et al. 2011a,b).…”

Section: Methodsmentioning

confidence: 99%

“…Mannose‐6‐phosphate (M6P) is a TGF‐β inhibitor that works by interfering with the conversion of latent forms of the TGF‐βs into their active form, and has been shown to have a marked anti‐scarring effect (McCallion & Ferguson, 1996). In recent studies on mice we used electrophysiological and histochemical techniques to screen the effect of a series of scar‐reducing agents, including different concentrations of M6P, applied at a sciatic nerve repair site (Ngeow et al. 2011a).…”

Section: Introductionmentioning

confidence: 99%

“…2011a). We demonstrated that intra‐neural and peri‐neural application of 600 m m M6P was the most effective agent for enhancing nerve regeneration (Ngeow et al. 2011a), and a subsequent study revealed benefits in early functional recovery (Ngeow et al.…”

Section: Introductionmentioning

confidence: 99%

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Histomorphometric changes in repaired mouse sciatic nerves are unaffected by the application of a scar-reducing agent

et al. 2011

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Microsurgical repair of transected peripheral nerves is compromised by the formation of scar tissue and the development of a neuroma, thereby limiting the success of regeneration. The aim of this study was to quantify histomorphometrically the structural changes in neural tissue that result from repair, and determine the effect of mannose-6-phosphate (M6P), a scar-reducing agent previously shown to enhance regeneration. In anaesthetised C57-black-6 mice, the left sciatic nerve was sectioned and repaired using four epineurial sutures. Either 100 lL of 600 mM M6P (five animals) or 100 lL of phosphate-buffered saline (placebo controls, five animals) was injected into and around the nerve repair site. A further group acted as sham-operated controls. After recovery for 6 weeks, the nerve was harvested for analysis using light and electron microscopy. Analysis revealed that when compared with sham controls, myelinated axons had smaller diameters both proximal and distal to the repair. Myelinated axon counts, axonal density and size all decreased across the repair site. There were normal numbers and densities of non-myelinated axons both proximal and distal to the repair. However, there were more Remak bundles distal to the repair site, and fewer non-myelinated axons per Remak bundle. Application of M6P did not affect any of these parameters.

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Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Histomorphometric changes in repaired mouse sciatic nerves are unaffected by the application of a scar-reducing agent

et al. 2011

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“…1,11,[16][17][18][19][20] This model is useful for testing the efficacy of new therapies for accelerating and enhancing axon growth and regeneration after injury. When compared with other models of peripheral nerve injury, sciatic nerve crush has several advantages, including easy access to the sciatic nerve.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the rate of spinal motor axon regeneration by choline acetyltransferase immunohistochemistry following sciatic nerve crush injury in mice

Yuan

Chiu³

et al. 2014

JNS

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Object. The purpose of this study was to examine whether choline acetyltransferase (ChAT) staining can be used for assessing the rate of motor neuron regeneration at an early phase of axon outgrowth.Methods. The authors developed a new sciatic nerve crush model in adult mice. In this model, in addition to performing a sciatic nerve crush injury, the authors excised the ipsilateral lumbar L3-6 dorsal root ganglion (DRG), which resulted in degeneration of the sensory fibers entering into the sciatic nerve. Crushed nerve sections obtained at Day 3 or Day 7 postinjury were analyzed by means of immunostaining.Results. The immunostaining showed that ChAT, a motor axon-specific antigen, was totally co-localized with growth-associated protein 43 (GAP-43), which is expressed in regenerating nerves and transported into growth cones.Conclusions. Our results suggest that measuring the length of motor axon outgrowth by ChAT immunostaining is reliable. ChAT staining provides a more convenient method for evaluating the rate of motor axon outgrowth in a mixed nerve. (http://thejns.org/doi/abs/10.3171/2013.8.JNS121648) KeY WorDS • nerve injury • axon regeneration • motor neuron • ChAT staining • peripheral nerveAbbreviations used in this paper: CGRP = calcitonin generelated peptide; ChAT = choline acetyltransferase; GAP-43 = growth-associated protein 43; NF200 = neurofilament-200; PBS = phosphate-buffered saline.

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Il10 and poly‐dl‐lactide‐ɛ‐caprolactone conduits in critical size nerve defect bridging—An experimental study

et al. 2015

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A comparison between the effects of three potential scar-reducing agents applied at a site of sciatic nerve repair

Cited by 12 publications

References 43 publications

Histomorphometric changes in repaired mouse sciatic nerves are unaffected by the application of a scar-reducing agent

Histomorphometric changes in repaired mouse sciatic nerves are unaffected by the application of a scar-reducing agent

Assessment of the rate of spinal motor axon regeneration by choline acetyltransferase immunohistochemistry following sciatic nerve crush injury in mice

Il10 and poly‐dl‐lactide‐ɛ‐caprolactone conduits in critical size nerve defect bridging—An experimental study

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