A comparison between neurally induced bone marrow derived mesenchymal stem cells and olfactory ensheathing glial cells to repair spinal cord injuries in rat

Oraee‐Yazdani, Saeed; Pedram, Mir Sepehr; Hafizi, Maryam; Kabiri, Mahboubeh; Soleimani, Masoud; Dehghan, Mohammad Mehdi; Jahanzad, Issa; Gheisari, Yousof; Hashemi, Seyed Mahmoud

doi:10.1016/j.tice.2012.03.003

Cited by 46 publications

(27 citation statements)

References 50 publications

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“…This is in accordance with other researchers [15,16] who used the movement curve analysis as proof for healing in sciatic nerve model and their results were also homogonous with their histological finding; however, others preferred to add electromyography to their assessment [17].…”

Section: Discussionsupporting

confidence: 89%

Angiogenesis induced by autologous whole bone marrow stem cells seeded on collagen scaffolds in silicone nerve tubes. An experimental study

Azeem

Al-Ahmady²,

Rahman

et al. 2014

Tanta Dental Journal

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Objective: This study aimed at evaluating Vascularity of tissues formed in silicone nerve tubes with collagen scaffolds seeded with Bone marrow stem cells (BMSC) as a sign of tissue regeneration. Materials and methods: Six adult mongrel dogs were included in this study; a 3 cm gap was made on the buccal branch of right side of facial nerve and also on the left side as a "control side". Next mononuclear cells containing mesenchymal stem cells were separated from whole bone marrow using Gradient separation method with Ficoll. Silicone tubes embedded with collagen type I sponge seeded with stem cells and neurogenic media were sutured in the perineural fascia of both ends of the cut nerve on the experimental side, while in the control side empty silicone tubes alone were sutured to the perineural fascia of the cut nerves. Healing was evaluated at 8 and 10 weeks scarifying 3 dogs each time and performing histological analysis. Results: After 8 weeks the 6 dogs showed limited movement in the facial area supplied by the buccal nerve on the experimental side and the salivary drooling decreased moderately. After 10 weeks the remaining dogs showed observable muscle movements and decreased salivary drooling in contrast to control side. These results go with the histological examination results showing massive multiple blood vessels formation and nerve like structures with partial absorption of the scaffold at 8 weeks then well organized nerve like tissues with dilated multiple blood vessels at 10 weeks. Conclusion: Collagen scaffolds seeded with mononuclear layer containing stem cells and nerve growth factors showed efficacy at achieving good neo-vascularization which lead to eventual good healing.

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Section: Discussionsupporting

confidence: 89%

Angiogenesis induced by autologous whole bone marrow stem cells seeded on collagen scaffolds in silicone nerve tubes. An experimental study

Azeem

Al-Ahmady²,

Rahman

et al. 2014

Tanta Dental Journal

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“…Many kinds of cells have been used in transplantation, including BMSCs, embryonic stem cells, neural stem cells, autologous olfactory ensheathing cells, human umbilical cord blood cells (HUCBCs), and Schwann cells . Among these types of cells, BMSCs are the strongest candidates due to the greater availability and weaker immunogenicity …”

Section: Discussionmentioning

confidence: 99%

Bone marrow mesenchymal stem cells stimulated with low‐intensity pulsed ultrasound: Better choice of transplantation treatment for spinal cord injury

Ning

Song

et al. 2018

CNS Neurosci Ther

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Summary Stem cell transplantation, especially treatment with bone marrow mesenchymal stem cells (BMSCs), has been considered a promising therapy for the locomotor and neurological recovery of spinal cord injury (SCI) patients. However, the clinical benefits of BMSCs transplantation remain limited because of the considerably low viability and inhibitory microenvironment. In our research, low‐intensity pulsed ultrasound (LIPUS), which has been widely applied to clinical applications and fundamental research, was employed to improve the properties of BMSCs. The most suitable intensity of LIPUS stimulation was determined. Furthermore, the optimized BMSCs were transplanted into the epicenter of injured spinal cord in rats, which were randomized into four groups: (a) Sham group (n = 10), rats received laminectomy only and the spinal cord remained intact. (b) Injury group (n = 10), rats with contused spinal cord subjected to the microinjection of PBS solution. (c) BMSCs transplantation group (n = 10), rats with contused spinal cord were injected with BMSCs without any priming. (d) LIPUS‐BMSCs transplantation group (n = 10), BMSCs stimulated with LIPUS were injected at the injured epicenter after contusion. Rats were then subjected to behavioral tests, immunohistochemistry, and histological observation. It was found that BMSCs stimulated with LIPUS obtained higher cell viability, migration, and neurotrophic factors expression in vitro. The rate of apoptosis remained constant. After transplantation of BMSCs and LIPUS‐BMSCs postinjury, locomotor function was significantly improved in LIPUS‐BMSCs transplantation group with higher level of brain‐derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in the epicenter, and the expression of neurotrophic receptor was also enhanced. Histological observation demonstrated reduced cavity formation in LIPUS‐BMSCs transplantation group when comparing with other groups. The results suggested LIPUS can improve BMSCs viability and neurotrophic factors expression in vitro, and transplantation of LIPUS‐BMSCs could promote better functional recovery, indicating possible clinical application for the treatment of SCI.

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“…Various stem cells, such as multipotent adult progenitor, mesenchymal, haemopoietic, umbilical cord blood (UCB), and embryonic stem cells (ESCs), have the potency to differentiate into the neural cells [7][8][9][10][11][12][13]. Some experimental studies have demonstrated the potential of restoring functional motor activity after transplanting motor neurons derived from neural stem cells (NSCs) and mesenchymal stem cells (MSCs) in paralyzed animals [14,15]. Unfortunately, NSCs transplanted into the spinal cord have much tendency to differentiate into either oligodendrocytes or astrocytes and rarely undergo differentiation into neuronal lineages [16].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Capability of Human Bone Marrow Mesenchymal Stem Cells and Endometrial Stem Cells to Differentiate into Motor Neurons on Electrospun Poly(ε-caprolactone) Scaffold

et al. 2015

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Human endometrial and bone marrow-derived mesenchymal stem cells can be differentiated into a number of cell lineages. Mesenchymal stem cells (MSCs) are potential candidates for cellular therapy. The differentiation of human bone marrow MSCs (hBM-MSCs) and endometrial stem cells (hEnSCs) into motor neuron-like cells has been rarely investigated previously; however, the comparison between these stem cells when they are differentiated into motor neuron-like cell is yet to be studied. The aim of this study was therefore to investigate and compare the capability of hBM-MSCs and hEnSCs cultured on tissue culture polystyrene (TCP) and poly ε-caprolactone (PCL) nanofibrous scaffold to differentiate into motor neuron-like cells in the presence of neural inductive molecules. Engineered hBM-MSCs and hEnSCs seeded on PCL nanofibrous scaffold were differentiated into beta-tubulin III, islet-1, Neurofilament-H (NF-H), HB9, Pax6, and choactase-positive motor neurons by immunostaining and real-time PCR, in response to the signaling molecules. The data obtained from PCR and immunostaining showed that the expression of motor neuron markers of both hBM-MSCs and hEnSCs differentiated cells on PCL scaffold are significantly higher than that of the control group. The expression of these markers in hEnSCs differentiated cells was higher than that in hBM-MSCs. However, this difference was not statistically significant. In conclusion, differentiated hBM-MSCs and hEnSCs on PCL can provide a suitable three-dimensional situation for neuronal survival and outgrowth for regeneration of the central nervous system. Both cells may be potential candidates for cellular therapy in motor neuron disorders. However, differentiation of hEnSCs into motor neuron-like cells was better than hBM-MSCs.

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A comparison between neurally induced bone marrow derived mesenchymal stem cells and olfactory ensheathing glial cells to repair spinal cord injuries in rat

Cited by 46 publications

References 50 publications

Angiogenesis induced by autologous whole bone marrow stem cells seeded on collagen scaffolds in silicone nerve tubes. An experimental study

Angiogenesis induced by autologous whole bone marrow stem cells seeded on collagen scaffolds in silicone nerve tubes. An experimental study

Bone marrow mesenchymal stem cells stimulated with low‐intensity pulsed ultrasound: Better choice of transplantation treatment for spinal cord injury

Comparison of Capability of Human Bone Marrow Mesenchymal Stem Cells and Endometrial Stem Cells to Differentiate into Motor Neurons on Electrospun Poly(ε-caprolactone) Scaffold

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