2020
DOI: 10.1002/saj2.20118
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A comparison between fatty acid methyl ester profiling methods (PLFA and EL‐FAME) as soil health indicators

Abstract: Fatty acid methyl ester (FAME) profiling for characterizing microbial community composition typically is conducted via phospholipid fatty acid (PLFA) or ester‐linked fatty acid methyl ester (EL‐FAME) methods. As soil health assessments aim to be utilized across the nation and globe, the robust measurement and interpretation of microbial communities across a range of soils and environments will be necessary. This study compared PLFA and EL‐FAME methods for detecting and interpreting profiles of microbial commun… Show more

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Cited by 28 publications
(11 citation statements)
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“…However, EL-FAME has become popular due to its simplicity and because it is less time consuming than PLFA analysis (Miura, Makoto, Niwa, Kaneko, & Sakamoto, 2017;Schutter and Dick, 2000). Additionally, the extraction in EL-FAME is directly from soil samples, and thus, may contain other lipid fractions including neutral lipids (Zelles, 1999;Miura et al, 2017), which give the advantage of evaluating the arbuscular mycorrhizal fungi (AMF) marker 16:1ω5c (Cotton & Acosta-Martínez, 2018;Li et al, 2020). The EL-FAME method has been shown to be sensitive at detecting changes in microbial communities under different management practices and regions (e.g., Mbuthia et al, 2015;Li et al, 2018), and after natural climatic disturbances such as drought, hurricanes, and extreme temperatures (Bérard, Bouchet, Sévenier, Pablo, & Gros, 2011; T A B L E 1 Quantitative polymerase chain reaction assays for genes associated with taxonomic groups and functional processes Cantrell et al, 2014;Pérez-Guzmán et al, 2020).…”
Section: Core Ideasmentioning
confidence: 99%
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“…However, EL-FAME has become popular due to its simplicity and because it is less time consuming than PLFA analysis (Miura, Makoto, Niwa, Kaneko, & Sakamoto, 2017;Schutter and Dick, 2000). Additionally, the extraction in EL-FAME is directly from soil samples, and thus, may contain other lipid fractions including neutral lipids (Zelles, 1999;Miura et al, 2017), which give the advantage of evaluating the arbuscular mycorrhizal fungi (AMF) marker 16:1ω5c (Cotton & Acosta-Martínez, 2018;Li et al, 2020). The EL-FAME method has been shown to be sensitive at detecting changes in microbial communities under different management practices and regions (e.g., Mbuthia et al, 2015;Li et al, 2018), and after natural climatic disturbances such as drought, hurricanes, and extreme temperatures (Bérard, Bouchet, Sévenier, Pablo, & Gros, 2011; T A B L E 1 Quantitative polymerase chain reaction assays for genes associated with taxonomic groups and functional processes Cantrell et al, 2014;Pérez-Guzmán et al, 2020).…”
Section: Core Ideasmentioning
confidence: 99%
“…Additionally, these methods allow for the identification of broad groups such as Gram‐positive, Gram‐negative, Actinobacteria, and saprophytic fungi (Willers, Jansen van Rensburg, & Claassens, 2015; Zelles, 1999). Recently, Li, Cano, Acosta‐Martínez, Veum, and Moore‐Kucera (2020) compared PLFA and EL‐FAME using 172 soil samples representing a wide range of physicochemical properties, and provided detailed information regarding equipment, costs, practical advantages, and results. For example, although certain FAME markers may be of plant origin, leading to slight overestimation of some groups, total FAMEs have been used as a proxy of microbial biomass (e.g., Li et al., 2018; Li et al., 2020).…”
Section: Introductionmentioning
confidence: 99%
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“…This contrasts neutral lipid fatty acids (NLFA), which are storage components, especially in fungi, and give interesting information on the nutritional status of fungi (Bååth 2003). Total fatty acid methyl ester (FAME) or ester-linked (EL) total FAME have been repeatedly determined in soil by in situ hydrolysis and methylation reactions of fatty acids, without the PLFA extraction step (Acosta-Martínez et al 2010;Li et al 2020). Total FAME and EL-FAME give similar information on the composition of the main microbial groups to that provided by PLFA (Acosta-Martínez et al 2010) but create an additional link to the formation of microbial necromass from decaying biomass by different microbial groups (Miltner et al 2012).…”
Section: Introductionmentioning
confidence: 99%
“…Despite the growing popularity of next-generation sequencing methods, which allow for detailed analysis of soil microbial communities, the determination of soil organisms' fatty acid patterns is still one of the most commonly used methods for testing microbial biomass and community structures [16]. Ester-linked fatty acid methyl ester (EL-FAME) profiling provides a rapid, inexpensive, reproducible, and reliable approach to characterize the existing microbial community of soil.…”
Section: Introductionmentioning
confidence: 99%