A Comparison and Analysis of Several Ways to Promote Haematin (Haem) Polymerisation and an Assessment of Its Initiation In Vitro

Hematin Polymerization Assay as a High-Throughput Screen for Identification of New Antimalarial Pharmacophores

“…It has become increasingly apparent over recent years that chloroquine most likely exerts its antimalarial activity through interaction with hematin in the lysosomal digestive vacuole of the malaria parasite (4,9,17,24). It is believed that this interaction affects the ability of the parasite to adequately sequester the toxic hematin that is released during the process of proteolytic degradation of hemoglobin into hemozoin, an inert pigment that develops within the parasite digestive vacuole during its growth within the erythrocyte.…”

“…It is believed that this interaction affects the ability of the parasite to adequately sequester the toxic hematin that is released during the process of proteolytic degradation of hemoglobin into hemozoin, an inert pigment that develops within the parasite digestive vacuole during its growth within the erythrocyte. There is some dispute as to whether this sequestration or polymerization is protein mediated (22,25) or not (2,7,9,14,15).…”

Biochemical Screening of Penaeus vannamei Shell Waste by Liquid Chromatography-Tandem Mass Spectrometry

“…Ogubona et al [18] reported that desethylchloroquine drug and its metabolites are secreted in the human saliva. The research findings of Dorn et al [19] proves that desethylchloroquine possess antiplasmodic activity [19]. Nefopam N-oxide was confirmed by the base peak found at the retention time 8.011 with the ESI-MS spectrum at m/z 269.14 ( Fig.…”

Biomimetic Approaches to Understanding the Mechanism of Haemozoin Formation

“…Even long before the recent discoveries of the relationship between haemozoin and lipids, there had been a number of studies suggesting that lipids mediate its formation. The proposal was first made by Bendrat et al (1995) and subsequently supported by Dorn et al who showed that an acetonitrile extract of P. falciparum trophozoites supports haemozoin formation (Dorn et al 1998). Fitch and co-workers also demonstrated that chloroform extracts of infected and uninfected red blood cells, monooleoylglycerol (MOG) and DOG as well as certain fatty acids and detergents efficiently support its formation (Fitch et al 1999).…”